Protein Information

Name laminin (protein family or complex)
Synonyms Laminin; Laminins

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
16236823 Vainionpaa N, Kikkawa Y, Lounatmaa K, Miner JH, Rousselle P, Virtanen I: Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells. Am J Physiol Cell Physiol. 2006 Mar;290(3):C764-75. Epub 2005 Oct 19.

When the protein synthesis during the adhesion was inhibited with cycloheximide, the formation of fibrillar adhesions on laminin-10 was abolished, suggesting that laminin-10 does not stimulate the formation of any adhesion structures.
7(0,0,0,7) Details
16180102 Jiang Y, Cheng DW, Crook ED, Singh LP: Transforming growth factor-beta1 regulation of laminin gamma1 and fibronectin expression and survival of mouse mesangial cells. Mol Cell Biochem. 2005 Oct;278(1-2):165-75.

6(0,0,0,6) Details
17259394 Mariappan MM, Feliers D, Mummidi S, Choudhury GG, Kasinath BS: High glucose, high insulin, and their combination rapidly induce laminin-beta1 synthesis by regulation of mRNA translation in renal epithelial cells. Diabetes. 2007 Feb;56(2):476-85.

Cycloheximide, but not actinomycin-D, abrogated increased laminin-beta1 synthesis.
5(0,0,0,5) Details
16282198 Chao JT, Martinez-Lemus LA, Kaufman SJ, Meininger GA, Ramos KS, Wilson E: Modulation of alpha7-integrin-mediated adhesion and expression by platelet-derived growth factor in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2006 Apr;290(4):C972-80. Epub 2005 Nov 9.

Actinomycin D and cycloheximide attenuated PDGF-induced increases in alpha (7)-integrin, indicating the involvement of de novo mRNA and protein synthesis.
PDGF-treated VSMC adhered to laminin more efficiently (42 +/- 6% increase, P < 0.01), and this increase was partially inhibited by anti-alpha (7)-integrin function-blocking antibody.
2(0,0,0,2) Details
16817231 Siri S, Chen MJ, Chen TT: Biological activity of rainbow trout Ea4-peptide of the pro-insulin-like growth factor (pro-IGF)-I on promoting attachment of breast cancer cells (MDA-MB-231) via alpha2- and beta1-integrin. J Cell Biochem. 2006 Dec 15;99(6):1524-35.

In addition, rtEa4-peptide induced the expression of fibronectin 1 and laminin receptor genes in MDA-MB-231 cells.
Blocking new protein synthesis by cycloheximide significantly reduced the attachment of MDA-MB-231 cells to rtEa4-peptide coated wells by 50%.
2(0,0,0,2) Details
19664126 Misiak-Tloczek A, Brzezinska-Blaszczyk E: IL-6, but not IL-4, stimulates chemokinesis and TNF stimulates chemotaxis of tissue mast cells: involvement of both mitogen-activated protein kinases and phosphatidylinositol 3-kinase signalling pathways. APMIS. 2009 Aug;117(8):558-67.


Under the same experimental conditions, mast cells were shown to migrate in response to IL-6 stimulation in the presence of laminin.
1(0,0,0,1) Details