| Name | mitogen activated protein kinase (protein family or complex) |
|---|---|
| Synonyms | MAPK; mitogen activated protein kinase; mitogen activated protein kinases |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 16452486 | Seymour KJ, Roberts LE, Fini MA, Parmley LA, Oustitch TL, Wright RM: Stress activation of mammary epithelial cell xanthine oxidoreductase is mediated by p38 MAPK and CCAAT/enhancer-binding protein-beta. J Biol Chem. 2006 Mar 31;281(13):8545-58. Epub 2006 Feb 1. We demonstrate here that XOR was induced in HC11 cells by low dose cycloheximide and that expression was blocked by inhibitors of p38 MAPK. |
34(0,1,1,4) | Details |
| 19657054 | Tew SR, Peffers MJ, McKay TR, Lowe ET, Khan WS, Hardingham TE, Clegg PD: Hyperosmolarity regulates SOX9 mRNA posttranscriptionally in human articular chondrocytes. Am J Physiol Cell Physiol. 2009 Oct;297(4):C898-906. Epub 2009 Aug 5. We previously showed that activation of p38 MAPK by cycloheximide in human chondrocytes leads to stabilization of SOX9 mRNA (Tew SR and Hardingham TE. |
33(0,1,1,3) | Details |
| 18767967 | Scott DW, Longpre JM, Loo G: Upregulation of GADD153 by involvement of MAPK. . DNA Cell Biol. 2008 Nov;27(11):607-14. -induced upregulation of GADD153 mRNA was attenuated by actinomycin D, but apparently not by cycloheximide. |
2(0,0,0,2) | Details |
| 15608616 | Buschbeck M, Hofbauer S, Di Croce L, Keri G, Ullrich A: Abl-kinase-sensitive levels of ERK5 and its intrinsic basal activity contribute to leukaemia cell survival. EMBO Rep. 2005 Jan;6(1):63-9. It is well established that the mitogen-activated protein kinase (MAPK) signal is regulated through phosphorylation-dependent activation by the three-tiered MAPK cascade. |
2(0,0,0,2) | Details |
| 18440740 | Song HJ, Shin CY, Oh TY, Sohn UD: The protective effect of eupatilin on indomethacin-induced cell damage in cultured feline ileal smooth muscle cells: involvement of HO-1 and ERK. J Ethnopharmacol. 2008 Jun 19;118(1):94-101. Epub 2008 Mar 22. We also found that PD98059, a MEK (MAPK/ERK kinase) inhibitor, attenuated the eupatilin-induced HO-1 expression and nuclear translocation of transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2). Pretreatment of ISMC with cycloheximide, an inhibitor of protein synthesis, attenuated the cytoprotective effect of eupatilin, suggesting that eupatilin induces proteins that are responsible for the cytoprotection. |
1(0,0,0,1) | Details |
| 19770273 | Steinmann J, Halldorsson S, Agerberth B, Gudmundsson GH: Phenylbutyrate induces antimicrobial peptide expression. Antimicrob Agents Chemother. 2009 Dec;53(12):5127-33. Epub 2009 Sep 21. Furthermore, we have demonstrated that inhibition of the mitogen-activated protein kinases MEK1/2 and c-Jun N-terminal kinase attenuate PBA-induced gene expression. |
1(0,0,0,1) | Details |
| 17438304 | Sonalker PA, Jackson EK: via beta-adrenoceptors, regulates bumetanide-sensitive cotransporter type 1 expression in thick ascending limb cells. Hypertension. 2007 Jun;49(6):1351-7. Epub 2007 Apr 16. We conclude that regulation of bumetanide-sensitive cotransporter type 1 by proceeds via the beta-adrenoceptor receptor-cAMP-protein kinase A pathway that involves in part mitogen-activated protein kinases and that alpha-adrenoceptor activation negatively regulates bumetanide-sensitive cotransporter type 1 protein levels. Treatment with either alone or in the presence of actinomycin D or cycloheximide, had no effect on cotransporter mRNA levels. |
1(0,0,0,1) | Details |
| 15965068 | Hou CC, Hung SL, Kao SH, Chen TH, Lee HM: induces heme-oxygenase expression in glomerular mesangial cells. Ann N Y Acad Sci. 2005 May;1042:235-45. -induced HO-1 protein expression was inhibited by actinomycin D and cycloheximide, suggesting that de novo transcription and translation are required in this process. |
0(0,0,0,0) | Details |
| 19800419 | Hwangbo C, Lee HS, Park J, Choe J, Lee JH: The anti-inflammatory effect of tussilagone, from Tussilago farfara, is mediated by the induction of heme oxygenase-1 in murine macrophages. Int Immunopharmacol. 2009 Dec;9(13-14):1578-84. Epub 2009 Oct 1. TSL-mediated HO-1 protein induction was not inhibited by treatment with actinomycin D, a transcriptional inhibitor, but by cycloheximide, a translational inhibitor. |
0(0,0,0,0) | Details |
| 19583809 | Keating N, Mroz MS, Scharl MM, Marsh C, Ferguson G, Hofmann AF, Keely SJ: Physiological concentrations of bile acids down-regulate agonist induced secretion in colonic epithelial cells. J Cell Mol Med. 2009 Aug;13(8B):2293-303. Epub 2009 Jul 6. The EGFr inhibitor, AG1478, and the protein synthesis inhibitor, cycloheximide, reversed the antisecretory effects of DCA, while the MAPK inhibitors, PD98059 and SB203580, did not. |
32(0,1,1,2) | Details |
| 17194804 | Lee SY, Cherla RP, Tesh VL: Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1. Infect Immun. 2007 Mar;75(3):1291-302. Epub 2006 Dec 28. Finally, the protein synthesis inhibitors Stx1 and anisomycin triggered limited apoptosis and prolonged JNK and p38 MAPK activation, while macrophage-like cells treated with cycloheximide remained viable and showed transient activation of MAPKs. |
32(0,1,1,2) | Details |
| 19247210 | Kim J, Park S, Lee S, Choi S: Amygdala depotentiation ex vivo requires mitogen-activated protein kinases and protein synthesis. Neuroreport. 2009 Mar 25;20(5):517-20. U0126, a mitogen-activated protein kinase inhibitor, and cycloheximide, a protein synthesis inhibitor, blocked depotentiationex vivo. |
32(0,1,1,2) | Details |
| 16464966 | Balzary RW, Cocks TM: Lipopolysaccharide induces epithelium- and (2)-dependent relaxation of mouse isolated trachea through activation of cyclooxygenase (COX)-1 and COX-2. J Pharmacol Exp Ther. 2006 May;317(2):806-12. Epub 2006 Feb 7. The LPS antagonist polymixin B; the nonselective COX inhibitor indomethacin; the selective COX-1 and COX-2 inhibitors 5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole (SC560) and 4-[5-(4-chlorophenyl)-1-(trifluoromethyl)-1H-pyrazol-1-yl]-benzenesulfonam ide (SC236), respectively; the transcription inhibitor actinomycin D; the translation inhibitor cycloheximide; the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imadazole (SB203580); and a combination of the mixed DP/EP1/EP2 receptor antagonist 6-isopropoxy-9-xanthone-2-carboxylic acid (AH6809) and the EP4 receptor antagonist 4'-[3-butyl-5-oxo-1-(2-trifluoromethyl-phenyl)-1-5-dihydro-[1,2,4] triazol- 4-ylmethyl]-biphenyl-2- (3-methyl-thiophene-2-carbonyl)-amide (L-161982) all abolished relaxation to LPS, giving instead slowly developing, small contractions over 60 min. |
32(0,1,1,2) | Details |
| 17197193 | Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR: Cardiotrophin-1 induces interleukin-6 synthesis in human umbilical vein endothelial cells. Cytokine. 2006 Nov;36(3-4):101-6. Epub 2007 Jan 2. For the pathway determination following inhibitors were used: (signal transducer and activation of transcription (STAT) 3 phosphorylation), wortmannin (phosphatiylinositol 3-kinase (PI3K)), SB203580 (p38 mitogen-activated protein kinase (MAPK)), AG490 (Janus kinase (JAK) 2), PD98059 (mitogen-activated protein kinase kinase (MEK) 1/2), parthenolide (nuclear factor kappaB) and cycloheximide (protein biosynthesis). |
31(0,1,1,1) | Details |
| 16040608 | Verriere VA, Hynes D, Faherty S, Devaney J, Bousquet J, Harvey BJ, Urbach V: Rapid effects of dexamethasone on intracellular pH and Na+/H+ exchanger activity in human bronchial epithelial cells. J Biol Chem. 2005 Oct 28;280(43):35807-14. Epub 2005 Jul 22. The rapid effect of dexamethasone on pH (i) was neither affected by the inhibitor of transcription, cycloheximide, nor by the classical glucocorticoid and mineralocorticoid receptors antagonists, RU486 and spironolactone, respectively. By using a PepTag assay system and Western blotting, we have shown that dexamethasone stimulated cAMP-dependent protein kinase and mitogen-activated protein kinase activities. |
2(0,0,0,2) | Details |
| 15531589 | Gerhard R, Tatge H, Genth H, Thum T, Borlak J, Fritz G, Just I: Clostridium difficile toxin A induces expression of the stress-induced early gene product RhoB. J Biol Chem. 2005 Jan 14;280(2):1499-505. Epub 2004 Nov 5. The up-regulation of RhoB was approximately 15-fold and was based on the de novo synthesis of the GTPase because cycloheximide completely inhibited the toxin A effect. The p38 MAPK inhibitor SB202190 reduced the expression of RhoB, indicating a participation of the p38 MAPK in this stress response. |
1(0,0,0,1) | Details |
| 16087789 | Ishizawa K, Izawa Y, Ito H, Miki C, Miyata K, Fujita Y, Kanematsu Y, Tsuchiya K, Tamaki T, Nishiyama A, Yoshizumi M: stimulates vascular smooth muscle cell proliferation via big mitogen-activated protein kinase 1 activation. Hypertension. 2005 Oct;46(4):1046-52. Epub 2005 Aug 8. -mediated phenomena were concluded to be attributable to a nongenomic effect because cycloheximide failed to inhibit -induced BMK1 activation. -induced nongenomic effects are attributable in part to the activation of extracellular signal-regulated kinase 1/2 (ERK1/2), a classical mitogen-activated protein (MAP) kinase. |
1(0,0,0,1) | Details |
| 16298406 | Urbach V, Verriere V, Grumbach Y, Bousquet J, Harvey BJ: Rapid anti-secretory effects of glucocorticoids in human airway epithelium. Steroids. 2006 Apr;71(4):323-8. Epub 2005 Nov 18. The rapid responses to glucocorticoid were reduced by the inhibitors of adenylated cyclase, cAMP-dependent protein kinase (PKA) and mitogen-activated protein kinase (ERK1/2). The rapid effects of dexamethasone on intracellular pH and Ca (2+) were not affected by inhibitors of transcription, cycloheximide or by the classical glucocorticoid and mineralocorticoid receptors antagonists, RU486 and spironolactone, respectively. |
1(0,0,0,1) | Details |
| 18708082 | Luo SF, Lin CC, Chen HC, Lin WN, Lee IT, Lee CW, Hsiao LD, Yang CM: Involvement of MAPKs, NF-kappaB and p300 co-activator in IL-1beta-induced cytosolic phospholipase A2 expression in canine tracheal smooth muscle cells. Toxicol Appl Pharmacol. 2008 Nov 1;232(3):396-407. Epub 2008 Jul 29. IL-1beta-induced cPLA2 expression and production was inhibited by actinomycin D and cycloheximide, indicating the involvement of transcriptional and translational events in these responses. |
0(0,0,0,0) | Details |
| 16704410 | Harrison EM, McNally SJ, Devey L, Garden OJ, Ross JA, Wigmore SJ: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells. FEBS J. 2006 Jun;273(11):2345-56. The induction of heme oxygenase-1 in renal adenocarcinoma cells was blocked by actinomycin D and cycloheximide and was abolished by the phosphatidylinositol 3-kinase inhibitor, LY294002, but not by the inactive analog LY303511. |
0(0,0,0,0) | Details |
| 16696851 | Tang HB, Inoue A, Iwasa M, Hide I, Nakata Y: Substance P release evoked by or from rat cultured dorsal root ganglion neurons is conversely modulated with bradykinin. J Neurochem. 2006 Jun;97(5):1412-8. Bradykinin-evoked SP release from cultured adult rat DRG neurons was attenuated by either the mitogen-activated protein kinase kinase (MEK) inhibitor (U0126) or cycloheximide. |
31(0,1,1,1) | Details |
| 19648110 | King EM, Holden NS, Gong W, Rider CF, Newton R: Inhibition of NF-kappaB-dependent transcription by MKP-1: transcriptional repression by glucocorticoids occurring via p38 MAPK. J Biol Chem. 2009 Sep 25;284(39):26803-15. Epub 2009 Jul 31. |
9(0,0,0,9) | Details |
| 17524467 | Nanassy L, Lee K, Javor A, Machaty Z: Changes in MPF and MAPK activities in porcine oocytes activated by different methods. Theriogenology. 2007 Jul 15;68(2):146-52. Epub 2007 May 23. Three activativation methods were tested: (1) electroporation (EP); (2) electroporation combined with I (BL), an inhibitor of cdc2 and cdk2 kinases; (3) electroporation followed by a treatment with cycloheximide (CHX), a protein synthesis blocker. |
6(0,0,0,6) | Details |
| 19188658 | Kulasekaran P, Scavone CA, Rogers DS, Arenberg DA, Thannickal VJ, Horowitz JC: Endothelin-1 and transforming growth factor-beta1 independently induce fibroblast resistance to apoptosis via AKT activation. Am J Respir Cell Mol Biol. 2009 Oct;41(4):484-93. Epub 2009 Feb 2. Activation of the PI3K/AKT pathway by ET-1 inhibits fibroblast apoptosis, and this inhibition is reversed by blockade of p38 MAPK or PI3K. |
2(0,0,0,2) | Details |
| 15569826 | Furst R, Brueckl C, Kuebler WM, Zahler S, Krotz F, Gorlach A, Vollmar AM, Kiemer AK: Atrial natriuretic peptide induces mitogen-activated protein kinase phosphatase-1 in human endothelial cells via Rac1 and NAD (P) H oxidase/Nox2-activation. Circ Res. 2005 Jan 7;96(1):43-53. Epub 2004 Nov 29. |
2(0,0,0,2) | Details |
| 15805247 | Cinar B, De Benedetti A, Freeman MR: Post-transcriptional regulation of the androgen receptor by Mammalian target of rapamycin. Cancer Res. 2005 Apr 1;65(7):2547-53. The signal to attenuate AR was mediated by the mammalian target of rapamycin, as shown by genetic and pharmacologic methods, and was independent of ErbB2/HER-2, extracellular signal-regulated kinase 1/2, and p38 mitogen-activated protein kinase pathways. |
1(0,0,0,1) | Details |
| 15736126 | Tan X, Wang YC, Sun QY, Peng A, Chen DY, Tang YZ: Effects of MAP kinase pathway and other factors on meiosis of Urechis unicinctus eggs. Mol Reprod Dev. 2005 May;71(1):67-76. Immunoblotting analysis using anti-ERK2 and anti-phospho-MAPK antibodies revealed a 44 kDa MAP kinase species that was constantly expressed in U. unicinctus eggs, quickly phosphorylated after fertilization, and dephosphorylated slowly before the completion of meiosis I. Phosphorylation of the protein was not depressed by protein synthesis inhibitor Cycloheximide (CHX), but was depressed by the MEK1 inhibitor PD98059. |
1(0,0,0,1) | Details |
| 16313754 | Wang XY, Li WQ, Lu J, Li N, Li JS: [Mechanism of reduction of albumin expression induced by lipopolysaccharide in rat hepatocytes]. Chin Med J. 2005 Oct 20;118(20):1695-702. The present study was designed to identify if the reduction of albumin expression is directly induced by LPS and modulated by activated extracellular signal-regulated protein kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) in rat hepatocytes. In another group, hepatocytes were pretreated with 100, 40 or 20 micromol/L of cycloheximide (CHX, an inhibitor of protein synthesis) for 30 minutes followed by 1 microg/ml LPS for 24 hours. |
1(0,0,0,1) | Details |
| 15666812 | Clark BJ, Li J: Janus kinase 2 signaling in the angiotensin II-dependent activation of StAR expression. Endocr Res. 2004 Nov;30(4):685-93. Using StAR promoter-reporter gene activity and pharmacological inhibition of signaling pathways, we have shown that Ang II-stimulated StAR transcription in H295R cells is dependent upon both influx of external Ca2+ and kinase signaling and is enhanced by protein kinase C and mitogen-activated protein kinase (ERK1/2) activation. The Ang II-dependent, but not (Bu) 2cAMP-dependent, induction of StAR mRNA was also blocked by AG490 and shown to be sensitive to cycloheximide treatment. |
1(0,0,0,1) | Details |
| 15557226 | Rahaus M, Desloges N, Wolff MH: Replication of varicella-zoster virus is influenced by the levels of JNK/SAPK and p38/MAPK activation. J Gen Virol. 2004 Dec;85(Pt 12):3529-40. Blocking gene expression by treating cells with actinomycin D or cycloheximide prior to infection resulted in activation of neither JNK/SAPK nor p38/MAPK. |
5(0,0,0,5) | Details |
| 20210885 | Ebeling S, Labudda A, Meinecke B: In Vitro Ageing of Porcine Oocytes: Changes in Phosphorylation of the Mitogen-Activated Protein Kinase (MAPK) and Parthenogenetic Activability. Reprod Domest Anim. 2010 Mar 2. After parthenogenetic activation of oocytes by /cycloheximide, cleavage stages were reached with rates of 51.9% (46 h IVM), 42.0% (12 h ageing) and 40.3% (26 h ageing), respectively. |
5(0,0,0,5) | Details |
| 16506055 | Doronzo G, Russo I, Mattiello L, Riganti C, Anfossi G, Trovati M: Insulin activates hypoxia-inducible factor-1alpha in human and rat vascular smooth muscle cells via phosphatidylinositol-3 kinase and mitogen-activated protein kinase pathways: impairment in insulin resistance owing to defects in insulin signalling. Diabetologia. 2006 May;49(5):1049-63. Epub 2006 Feb 28. The insulin-induced increase of HIF-1alpha is blunted by the translation inhibitor cycloheximide, LY294002, PD98059, SP600125 and rapamycin, but not by SB203580. |
2(0,0,0,2) | Details |
| 18787018 | Adams BD, Claffey KP, White BA: Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 2009 Jan;150(1):14-23. Epub 2008 Sep 11. Half-life studies using cycloheximide indicated that EGF enhanced, whereas U0126 decreased, Ago2 protein stability. |
2(0,0,0,2) | Details |
| 18234961 | Chetoui N, Sylla K, Gagnon-Houde JV, Alcaide-Loridan C, Charron D, Al-Daccak R, Aoudjit F: Down-regulation of mcl-1 by small interfering RNA sensitizes resistant melanoma cells to fas-mediated apoptosis. Mol Cancer Res. 2008 Jan;6(1):42-52. Thus, our study identifies mitogen-activated protein kinase/ERK/Mcl-1 as an important survival signaling pathway in the resistance of melanoma cells to Fas-mediated apoptosis and suggests that its targeting may contribute to the elimination of melanoma tumors by the immune system. In this study, we report that treatment of Fas-resistant melanoma cell lines with cycloheximide, a general inhibitor of de novo protein synthesis, sensitizes them to anti-Fas monoclonal antibody (mAb)-induced apoptosis. |
1(0,0,0,1) | Details |
| 17287395 | Balestreire EM, Apodaca G: Apical epidermal growth factor receptor signaling: regulation of stretch-dependent exocytosis in bladder umbrella cells. Mol Biol Cell. 2007 Apr;18(4):1312-23. Epub 2007 Feb 7. Long-term changes in apical exocytosis depended on protein synthesis, which occurred upon EGF receptor-dependent activation of mitogen-activated protein kinase signaling. |
1(0,0,0,1) | Details |
| 16514187 | Endo T, Naito K, Kume S, Nishimura Y, Kashima K, Tojo H: Activities of maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK) are not required for the global histone deacetylation observed after germinal vesicle breakdown (GVBD) in porcine oocytes. Reproduction. 2006 Mar;131(3):439-47. In the present study, we examined the involvement of a crucial cell-cycle-regulator, maturation-promoting factor (MPF), and a meiosis-related kinase, mitogen-activated protein kinase (MAPK), in the global histone deacetylation during porcine oocyte maturation. This deacetylation after AGVD was not affected by an MPF inhibitor, roscovitine, or an inhibitor of protein synthesis, cycloheximide, but was completely prevented by an inhibitor of histone deactylases (HDACs), trichostatine A. |
4(0,0,0,4) | Details |
| 19289468 | Kurada BR, Li LC, Mulherkar N, Subramanian M, Prasad KV, Prabhakar BS: MADD, a splice variant of IG20, is indispensable for MAPK activation and protection against apoptosis upon tumor necrosis factor-alpha treatment. J Biol Chem. 2009 May 15;284(20):13533-41. Epub 2009 Mar 16. Abrogation of MADD expression rendered cells highly susceptible to TNFalpha-induced apoptosis in the absence of cycloheximide. |
4(0,0,0,4) | Details |
| 16051672 | Moeller LC, Dumitrescu AM, Refetoff S: Cytosolic action of thyroid hormone leads to induction of hypoxia-inducible factor-1alpha and glycolytic genes. Mol Endocrinol. 2005 Dec;19(12):2955-63. Epub 2005 Jul 28. HIF-1alpha expression and activity can be regulated through phosphatidylinositol-OH-3-kinase (PI3K) and MAPK signaling; thus the possibility of alternative, nonnuclear pathways of TH action was raised. |
2(0,0,0,2) | Details |
| 17373649 | Li JP, Yang JL: Cyclin B1 proteolysis via p38 MAPK signaling participates in G2 checkpoint elicited by arsenite. J Cell Physiol. 2007 Aug;212(2):481-8. |
2(0,0,0,2) | Details |
| 16581040 | Xiong W, Kojic LZ, Zhang L, Prasad SS, Douglas R, Wang Y, Cynader MS: Anisomycin activates p38 MAP kinase to induce LTD in mouse primary visual cortex. Brain Res. 2006 Apr 26;1085(1):68-76. Epub 2006 Apr 11. In contrast, two other protein synthesis inhibitors, emetine and cycloheximide, have no effect either on baseline synaptic transmission or on LTD. These results indicate that activation of p38 MAPK by anisomycin induces LTD and subsequently occludes electrically induced LTD. |
2(0,0,0,2) | Details |
| 18029162 | Sampieri CL, Nuttall RK, Young DA, Goldspink D, Clark IM, Edwards DR: Activation of p38 and JNK MAPK pathways abrogates requirement for new protein synthesis for phorbol ester mediated induction of select MMP and TIMP genes. Matrix Biol. 2008 Mar;27(2):128-38. Epub 2007 Oct 4. The requirement for ongoing translation was analysed using three protein synthesis inhibitors, anisomycin, cycloheximide and emetine. |
1(0,0,0,1) | Details |
| 17138965 | Sun J, Fegan PE, Desai AS, Madara JL, Hobert ME: Flagellin-induced tolerance of the Toll-like receptor 5 signaling pathway in polarized intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007 Mar;292(3):G767-78. Epub 2006 Nov 30. Prolonged flagellin exposure blocks activation of the NF-kappaB, MAPK, and phosphoinositol 3-kinase signaling pathways and results in the internalization of a fraction of the basolateral TLR5 without affecting the polarity or total expression of TLR5. |
1(0,0,0,1) | Details |
| 20339118 | Montezano AC, Burger D, Paravicini TM, Chignalia AZ, Yusuf H, Almasri M, He Y, Callera GE, He G, Krause KH, Lambeth D, Quinn MT, Touyz RM: Reduced Oxidase 5 (Nox5) Regulation by Angiotensin II and Endothelin-1 Is Mediated via /Calmodulin-Dependent, Rac-1-Independent Pathways in Human Endothelial Cells. Circ Res. 2010 Mar 25. Objectives: We sought to test whether vasoactive agents regulate Nox5 through Ca (2+)/calmodulin-dependent processes and whether Ca (2+)-sensitive Nox5, associated with Rac-1, generates (O2 (.-)) and activates growth and inflammatory responses via mitogen-activated protein kinases in human endothelial cells (ECs). Effects were inhibited by actinomycin D and cycloheximide and blunted by calmidazolium and low extracellular Ca (2+) ([Ca (2+)] e). |
1(0,0,0,1) | Details |
| 19330094 | Iwahashi Y, Kitagawa E, Iwahashi H: Analysis of mechanisms of T-2 toxin toxicity using yeast DNA microarrays. Int J Mol Sci. 2008 Dec;9(12):2585-600. Epub 2008 Dec 11. Interestingly, T-2 toxin-induced yeast gene expression profiles were found to be similar to profiles obtained following cycloheximide treatment. Moreover, T-2 toxin treatment was found to activate facilitators, gluconeogenesis and cell arrest related genes such as mitogen-activated protein kinase genes (FUS3). |
1(0,0,0,1) | Details |
| 17429344 | Lee HT, Kim M, Jan M, Penn RB, Emala CW: Renal tubule necrosis and apoptosis modulation by A1 receptor expression. Kidney Int. 2007 Jun;71(12):1249-61. Epub 2007 Apr 11. Receptor-overexpressing cells were protected against peroxide-induced necrosis and tumor necrosis factor-alpha/cycloheximide-induced apoptosis. Overexpression of the receptor resulted in a significantly higher baseline expression of both total and phosphorylated heat-shock protein (HSP) 27; the latter due to A1 receptor enhancement of p38 and AP2 mitogen-activated protein kinase activities. |
1(0,0,0,1) | Details |
| 16330215 | Fujimoto S, Katsuki H, Kume T, Akaike A: Thrombin-induced delayed injury involves multiple and distinct signaling pathways in the cerebral cortex and the striatum in organotypic slice cultures. Neurobiol Dis. 2006 Apr;22(1):130-42. Epub 2005 Dec 5. These effects were prevented by cycloheximide and actinomycin D but not by a caspase-3 inhibitor. In addition, inhibition of extracelluar signal-regulated kinase (ERK), Src kinase and protein kinase C prevented both neuronal injury in the cortex and shrinkage of the striatum, whereas inhibition of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase prevented shrinkage of the striatum only. |
1(0,0,0,1) | Details |
| 15654867 | Duvarci S, Nader K, LeDoux JE: Activation of extracellular signal-regulated kinase- mitogen-activated protein kinase cascade in the amygdala is required for memory reconsolidation of auditory fear conditioning. Eur J Neurosci. 2005 Jan;21(1):283-9. Furthermore, we verified that reconsolidation requires translation using a second protein synthesis inhibitor, cycloheximide. |
4(0,0,0,4) | Details |
| 16308312 | Yeh CT, Yen GC: Involvement of p38 MAPK and Nrf2 in phenolic acid-induced P-form phenol sulfotransferase expression in human hepatoma HepG2 cells. Carcinogenesis. 2006 May;27(5):1008-17. Epub 2005 Nov 23. Actinomycin D and cycloheximide inhibited -responsive PST-P mRNA expression, indicating that is a requirement for transcription and de novo protein synthesis. |
4(0,0,0,4) | Details |
| 18506480 | Chen PY, Lee KT, Chi WC, Hirt H, Chang CC, Huang HJ: Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants. Planta. 2008 Aug;228(3):499-509. Epub 2008 May 28. In addition, heat pretreatment downregulated ROS production and mitogen-activated protein kinase (MAPK) activities, both of which can be greatly elicited by Cu (2+) stress in rice roots. |
2(0,0,0,2) | Details |
| 18172009 | Gilkes DM, Pan Y, Coppola D, Yeatman T, Reuther GW, Chen J: Regulation of MDMX expression by mitogenic signaling. Mol Cell Biol. 2008 Mar;28(6):1999-2010. Epub 2008 Jan 2. Activated K-Ras and insulin-like growth factor 1 induce MDMX expression at the transcriptional level through mechanisms that involve the mitogen-activated protein kinase and c-Ets-1 transcription factors. |
1(0,0,0,1) | Details |
| 19070612 | Cazarolli LH, Folador P, Moresco HH, Brighente IM, Pizzolatti MG, Silva FR: Mechanism of action of the stimulatory effect of -6-C-(2''-O-alpha-l-rhamnopyranosyl)-beta-L-fucopyranoside on 14C- uptake. Chem Biol Interact. 2009 May 15;179(2-3):407-12. Epub 2008 Nov 25. The effect of compound 1 on uptake was completely nullified by wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), RO318220, an inhibitor of protein kinase C (PKC), PD98059, a specific inhibitor of mitogen-activated protein kinase (MEK), cycloheximide, an inhibitor of protein synthesis, and colchicine, a microtubule-depolymerizing agent. The effect of compound 1 on uptake was completely nullified by wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), RO318220, an inhibitor of protein kinase C (PKC), PD98059, a specific inhibitor of mitogen-activated protein kinase (MEK), cycloheximide, an inhibitor of protein synthesis, and colchicine, a microtubule-depolymerizing agent. |
1(0,0,0,1) | Details |
| 17477937 | Lim W, Jung J, Surh Y, Inoue H, Lee Y: Hypertonic and induces COX-2 via different signaling pathways in mouse cortical collecting duct M-1 cells. Life Sci. 2007 May 8;80(22):2085-92. Epub 2007 Apr 1. The treatment also increased COX-2 mRNA accumulation in a cycloheximide-independent manner, suggesting that ongoing protein synthesis is not required for COX-2 induction. The COX-2-inductive effect of hypertonicity was inhibited by SB203580, indicating that the effect is mediated by p38 MAPK. |
1(0,0,0,1) | Details |
| 17706224 | Zhang W, Zhang Y, Edvinsson L, Xu CB: Up-regulation of thromboxane A2 receptor expression by lipid soluble smoking particles through post-transcriptional mechanisms. Atherosclerosis. 2008 Feb;196(2):608-16. Epub 2007 Aug 13. The present study was designed to test if lipid soluble smoking particles (DSP) enhance receptor (TP) expression in rat mesenteric arteries, and if intracellular mitogen-activated protein kinase (MAPK) pathways play a role. This up-regulation was not affected by a general transcriptional inhibitor actinomycin D, but was almost completely abolished by cycloheximide, a general translational inhibitor. |
1(0,0,0,1) | Details |
| 15728510 | Molnarfi N, Hyka-Nouspikel N, Gruaz L, Dayer JM, Burger D: The production of IL-1 receptor antagonist in IFN-beta-stimulated human monocytes depends on the activation of phosphatidylinositol 3-kinase but not of STAT1. J Immunol. 2005 Mar 1;174(5):2974-80. Canonically, IFN-beta signals through activation of JAK/STAT pathway, although PI3K and MAPK have also been involved. The use of cycloheximide and actinomycin D shows that sIL-1Ra was an immediate early gene induced by IFN-beta and that PI3K was controlling sIL-1Ra gene transcription. |
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| 19920324 | Ji L, Chauhan A, Chauhan V: Upregulation of Cytoplasmic Gelsolin, an Amyloid-beta-Binding Protein, Under Oxidative Stress Conditions: Involvement of Protein Kinase C. J Alzheimers Dis. 2009 Nov 17. Pretreatment of cells with cycloheximide (an inhibitor of protein synthesis) resulted in significant inhibition of H However, both H |
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| 18005266 | Ratthe C, Girard D: Investigation of the interleukin (IL)-4/IL-4 receptor system in promyelocytic leukaemia PLB-985 cells during differentiation toward neutrophil-like phenotype: mechanism involved in IL-4-induced SOCS3 protein expression. Br J Haematol. 2008 Jan;140(1):59-70. Epub 2007 Nov 12. Moreover, PLB-985 cells lost surface expression of IL-13Ralpha1 during differentiation, resulting in PLB-985D cells expressing only IL-4Ralpha fully responsive to IL-4, as judged by activation of mitogen-activated protein (MAP) kinases and Janus kinase 1. Utilization of catalase, cycloheximide and inhibitors showed that IL-4 induced SOCS3 by a mechanism dependent on a complete oxidase complex, protein synthesis and phosphorylation, but independent of production of reactive species. |
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| 17331500 | Bian ZM, Elner SG, Elner VM: Regulation of VEGF mRNA expression and protein secretion by TGF-beta2 in human retinal pigment epithelial cells. Exp Eye Res. 2007 May;84(5):812-22. Epub 2007 Jan 9. Moreover, TGF-beta2-induced RPE VEGF secretion was significantly reduced by inhibitors of mitogen-activated protein (MAP) kinase (MEK) (U0126), p38 (SB202190), c-Jun NH2-terminal kinase (JNK), Sp600125, protein tyrosine kinase (PTK) and phosphatidylinositol 3-kinase (PI3K) (Ly294002). Stimulation of VEGF expression by TGF-beta2 was blocked by cycloheximide, suggesting that de novo protein synthesis is required. |
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| 17893873 | Galan-Moya EM, Hernandez-Losa J, Aceves Luquero CI, de la Cruz-Morcillo MA, Ramirez-Castillejo C, Callejas-Valera JL, Arriaga A, Aranburo AF, Ramon y Cajal S, Silvio Gutkind J, Sanchez-Prieto R: c-Abl activates p38 MAPK independently of its kinase activity: Implications in cisplatin-based therapy. Int J Cancer. 2008 Jan 15;122(2):289-97. |
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| 17240354 | Sato F, Imaizumi T, Sashinami H, Yoshida H, Kusumi T, Mori F, Wakabayashi K, Nakane A, Satoh K, Kijima H: Upregulation of vascular endothelial growth factor by heat-killed Listeria monocytogenes in macrophages. Biochem Biophys Res Commun. 2007 Mar 9;354(2):608-12. Epub 2007 Jan 16. Pretreatment of cells with cycloheximide, a protein synthesis inhibitor, inhibited the induction of VEGF mRNA by HKLM. Induction of VEGF by HKLM was partially inhibited by treatment of cells with SB203580, a p38 mitogen-activated protein kinase (MAPK) inhibitor, or a neutralizing antibody against tumor necrosis factor-alpha (TNF-alpha). |
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| 19094210 | Zayed N, Li X, Chabane N, Benderdour M, Martel-Pelletier J, Pelletier JP, Duval N, Fahmi H: Increased expression of lipocalin-type prostaglandin D2 synthase in osteoarthritic cartilage. Arthritis Res Ther. 2008;10(6):R146. Epub 2008 Dec 18. The upregulation of L-PGDS by IL-1beta was blocked by the translational inhibitor cycloheximide, indicating that this effect is indirect, requiring de novo protein synthesis. The roles of de novo protein synthesis and of the signalling pathways mitogen-activated protein kinases (MAPKs), nuclear factor-kappa-B (NF-kappaB), and Notch were evaluated using specific pharmacological inhibitors. |
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| 18491380 | Motoki T, Sugiura Y, Matsumoto Y, Tsuji T, Kubota S, Takigawa M, Gohda E: Induction of hepatocyte growth factor expression by in human fibroblasts through MAPK activation. J Cell Biochem. 2008 Jul 1;104(4):1465-76. The protein synthesis inhibitor cycloheximide completely inhibited upregulation of HGF mRNA induced by but superinduced HGF mRNA expression upregulated by 12-O-tetradecanoylphorbol 13-acetate (TPA). |
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| 17081505 | Paul S, Olausson P, Venkitaramani DV, Ruchkina I, Moran TD, Tronson N, Mills E, Hakim S, Salter MW, Taylor JR, Lombroso PJ: The striatal-enriched protein tyrosine phosphatase gates long-term potentiation and fear memory in the lateral amygdala. Biol Psychiatry. 2007 May 1;61(9):1049-61. Epub 2006 Nov 1. Activation of the ERK pathway by the sequential recruitment of mitogen-activated protein kinases is well understood. |
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| 17501665 | Lambert C, Oury C, Dejardin E, Chariot A, Piette J, Malaise M, Merville MP, Franchimont N: Further insights in the mechanisms of interleukin-1beta stimulation of osteoprotegerin in osteoblast-like cells. J Bone Miner Res. 2007 Sep;22(9):1350-61. Post-transcriptional effects were examined by using cycloheximide and actinomycin D. Herein, we defined the mechanisms of IL-1beta stimulation of OPG focusing on the potential involvement of MAPK and NF-kappaB. |
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| 15944291 | Wojciechowski W, Li H, Marshall S, Dell'Agnola C, Espinoza-Delgado I: Enhanced expression of CD20 in human tumor B cells is controlled through ERK-dependent mechanisms. J Immunol. 2005 Jun 15;174(12):7859-68. The effect of bryostatin-1 on CD20 expression in non-Hodgkin's lymphoma cells was mediated through the MAPK kinase/ERK signal transduction pathway and involved protein kinase C, but was independent of p38 MAPK and was insensitive to dexamethasone. |
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| 16543731 | Heo JS, Han HJ: PKC and MAPKs pathways mediate EGF-induced stimulation of 2-deoxyglucose uptake in mouse embryonic stem cells. Cell Physiol Biochem. 2006;17(3-4):145-58. Epub 2006 Mar 14. SB 203580 [p38 mitogen activated protein kinase (MAPK) inhibitor] or PD 98059 (p44/42 MAPKs inhibitor) blocked EGF-induced increase of 2-DG uptake. Actinomycin D and cycloheximide completely blocked the effect of EGF on 2-DG uptake. |
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| 16434099 | Gonzalez TV, Farrant SA, Mantis NJ: Ricin induces IL-8 secretion from human monocyte/macrophages by activating the p38 MAP kinase pathway. Mol Immunol. 2006 Apr;43(11):1920-3. Epub 2006 Jan 24. As predicted from the literature, we observed elevated levels of p38 mitogen activated protein kinase (MAPK), a post-transcriptional regulator of IL-8, in 28SC cells as early as 3h after ricin exposure. |
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| 16644485 | Nieminen R, Lahti A, Jalonen U, Kankaanranta H, Moilanen E: JNK inhibitor SP600125 reduces COX-2 expression by attenuating mRNA in activated murine J774 macrophages. Int Immunopharmacol. 2006 Jun;6(6):987-96. Epub 2006 Feb 9. Cycloheximide (that is known to activate JNK) enhanced COX-2 expression and its effect was inhibited by SP600125. In the present study we investigated the role of c-Jun NH2-terminal kinase (JNK), a member of the mitogen-activated protein kinase (MAPK) family in COX-2 expression and prostaglandin (PG) E2 production in murine J774 macrophages activated by bacterial lipopolysaccharide (LPS). |
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| 17192279 | Chang CT, Wu MS, Tian YC, Chen KH, Yu CC, Liao CH, Hung CC, Yang CW: Enhancement of epithelial sodium channel expression in renal cortical collecting ducts cells by advanced glycation end products. Nephrol Dial Transplant. 2007 Mar;22(3):722-31. Epub 2006 Dec 27. This study analysed mitogen-activated protein kinases signalling pathways by western blotting. |
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| 19376214 | Ridley W, Matsuoka M: -induced cyclooxygenase-2 expression and production in A549 human pulmonary epithelial cells. Toxicol Lett. 2009 Aug 10;188(3):180-5. Epub 2009 Apr 17. NaF-induced accumulation of COX-2 transcript was abolished by actinomycin D, but not cycloheximide. Phosphorylated forms of mitogen-activated protein kinases (MAPKs)-including extracellular signal-regulated protein kinase (ERK), c-Jun NH (2)-terminal kinase, and p38-increased after NaF exposure, while treatment with the MAPK/ERK kinase inhibitor U0126 and the p38 inhibitor SB203580 markedly suppressed COX-2 expression. |
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| 15877607 | Kim HY, Kim HK, Kim JR, Kim HS: Upregulation of LPS-induced chemokine KC expression by 15-deoxy-delta12,14- in mouse peritoneal macrophages. Immunol Cell Biol. 2005 Jun;83(3):286-93. However, the synergistic effect of 15d-PGJ (2) was markedly inhibited when the macrophages were treated with a inhibitor of the mitogen-activated protein kinase (MAPK) signalling pathway, 2'-amino-3'-methoxyflavine (PD98059). |
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| 18410509 | Messina S, Molinaro G, Bruno V, Battaglia G, Spinsanti P, Di Pardo A, Nicoletti F, Frati L, Porcellini A: Enhanced expression of Harvey ras induced by serum deprivation in cultured astrocytes. J Neurochem. 2008 Jul;106(2):551-9. Epub 2008 Apr 12. The increase in Ha-Ras levels was sensitive to the protein synthesis inhibitor, cycloheximide, suggesting that serum deprivation increases translation of preformed Ha-Ras mRNA. The late decline in Ha-Ras levels observed after 60 min was prevented by the proteasome inhibitor, MG132, as well as by the selective mitogen-activated protein kinase (MAPK) inhibitor, PD98059. |
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| 17329956 | Matsumoto E, Hatanaka M, Bohgaki M, Maeda S: PKC pathway and ERK/MAPK pathway are required for induction of cyclin D1 and p21Waf1 during 12-o-tetradecanoylphorbol 13--induced differentiation of myeloleukemia cells. Kobe J Med Sci. 2006;52(6):181-94. Induction of cyclin D1 expression in TPA-treated HL60 cells was inhibited with protein kinase C (PKC) inhibitor bisindolylmaleimide I and mitogen activated protein kinase kinase (MEK) inhibitor PD98059. |
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| 15654655 | Sabbieti MG, Marchetti L, Gabrielli MG, Menghi M, Materazzi S, Menghi G, Raisz LG, Hurley MM: Prostaglandins differently regulate FGF-2 and FGF receptor expression and induce nuclear translocation in osteoblasts via MAPK kinase. Cell Tissue Res. 2005 Feb;319(2):267-78. Epub 2004 Dec 10. Pre-treatment with cycloheximide blocks nuclear labelling for FGF-2 in response to PGF (2alpha). |
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| 19179479 | Armstrong SP, Caunt CJ, McArdle CA: Gonadotropin-releasing hormone and protein kinase C signaling to ERK: spatiotemporal regulation of ERK by docking domains and dual-specificity phosphatases. Mol Endocrinol. 2009 Apr;23(4):510-9. Epub 2009 Jan 29. Screening a short inhibitory RNA library targeting 16 DUSPs (nuclear-inducible DUSPs, cytoplasmic ERK MAPK phosphatases, c-Jun N-terminal kinase/p38 MAPK phosphatases, and atypical DUSPs) revealed GnRH effects to be influenced by DUSPs 5, 9, 10, 16, and 3 (i.e. by each DUSP class). Cycloheximide caused more sustained effects of GnRH and phorbol ester on ppERK, suggesting termination by nuclear-inducible DUSPs. |
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| 20157239 | Ji L, Chauhan A, Chauhan V: Upregulation of cytoplasmic gelsolin, an amyloid-beta-binding protein, under oxidative stress conditions: involvement of protein kinase C. J Alzheimers Dis. 2010 Jan;19(3):829-38. Pretreatment of cells with cycloheximide (an inhibitor of protein synthesis) resulted in significant inhibition of H (2) O (2) induced c-gelsolin expression, suggesting the possible de novo synthesis of c-gelsolin in cells. However, both H (2) O (2) and staurosporine activated the mitogen-activated protein kinases (MAPKs), i.e., c-Jun N-terminal kinase, P38, and extracellular signal-regulated kinase. |
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| 16794604 | Centeno C, Repici M, Chatton JY, Riederer BM, Bonny C, Nicod P, Price M, Clarke PG, Papa S, Franzoso G, Borsello T: Role of the JNK pathway in -mediated excitotoxicity of cortical neurons. Cell Death Differ. 2007 Feb;14(2):240-53. Epub 2006 Jun 23. We then analyze the action of D-JNKI1 on four JNK targets containing a JNK-binding domain: MAPK-activating death domain-containing protein/differentially expressed in normal and neoplastic cells (MADD/DENN), MKK7, MKK4 and JNK-interacting protein-1 (IB1/JIP-1). |
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| 19411256 | Liu R, Zheng HQ, Zhou Z, Dong JT, Chen C: KLF5 promotes breast cell survival partially through fibroblast growth factor-binding protein 1-pERK-mediated dual specificity MKP-1 protein phosphorylation and stabilization. J Biol Chem. 2009 Jun 19;284(25):16791-8. Epub 2009 May 1. By cycloheximide chase assays, we found that KLF5 decreases MKP-1 protein degradation via activating the ERK signaling. Interestingly, a pro-survival phosphatase, dual specificity mitogen-activated protein kinase phosphatase 1 (MKP-1), is down-regulated by KLF5 ablation. |
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| 16740979 | Chen YH, Lee MJ, Chang HH, Hung PF, Kao YH: 17 beta- stimulates resistin gene expression in 3T3-L1 adipocytes via the estrogen receptor, extracellularly regulated kinase, and CCAAT/enhancer binding protein-alpha pathways. Endocrinology. 2006 Sep;147(9):4496-504. Epub 2006 Jun 1. Treatment with either actinomycin D or cycloheximide prevented E2-stimulated resistin mRNA expression, suggesting that the effect of E2 requires new mRNA and protein synthesis. Although E2 was shown to increase activities of the estrogen receptor (ER) and MAPK kinase 1 and the association of nuclear ER alpha and CCAAT/enhancer binding protein-alpha with the resistin gene promoter, signaling was demonstrated to be blocked by pretreatment with either ICI182780 or PD98059. |
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| 16988072 | Story DJ, Stephens JM: Modulation and lack of cross-talk between signal transducer and activator of transcription 5 and Suppressor of cytokine signaling-3 in insulin and growth hormone signaling in 3T3-L1 adipocytes. Obesity (Silver Spring). 2006 Aug;14(8):1303-11. RESEARCH METHODS AND PROCEDURES: Fully differentiated 3T3-L1 adipocytes were exposed to INS, growth hormone (GH), or both of these growth factors, and the activation of STAT5 proteins and mitogen-activated protein kinase was examined using phospho-specific antibodies. |
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| 18066586 | Lee MO, Cho K, Kim SH, Jeong SH, Kim JA, Jung YH, Shim J, Shibato J, Rakwal R, Tamogami S, Kubo A, Agrawal GK, Jwa NS: Novel rice OsSIPK is a multiple stress responsive MAPK family member showing rhythmic expression at mRNA level. Planta. 2008 Apr;227(5):981-90. Epub 2007 Dec 8. A time course (30-120 min) experiment using a variety of elicitors and stresses revealed that the OsSIPK mRNA is strongly induced by jasmonic acid (JA), (SA), ethephon, abscisic acid, cycloheximide (CHX), JA/SA + CHX, cantharidin, okadaic acid, peroxide, and cold stress (12 degrees C), but not with wounding by cut, gaseous pollutants ozone, and dioxide, high temperature, ultraviolet C irradiation, and drought. |
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| 17050539 | Tew SR, Hardingham TE: Regulation of SOX9 mRNA in human articular chondrocytes involving p38 MAPK activation and mRNA stabilization. J Biol Chem. 2006 Dec 22;281(51):39471-9. Epub 2006 Oct 18. Disruption of actin stress fibers using any of these redifferentiation stimuli also supported the superinduction of SOX9 by cycloheximide. |
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| 18162528 | Shankar K, Liu X, Singhal R, Chen JR, Nagarajan S, Badger TM, Ronis MJ: Chronic consumption leads to disruption of homeostasis associated with induction of renal -24-hydroxylase (CYP24A1). Endocrinology. 2008 Apr;149(4):1748-56. Epub 2007 Dec 27. In addition, inhibition of MAPK signaling pathways with the MAPK kinase inhibitor U0126 or the p38 inhibitor SB203580 inhibited EtOH induction of CYP24A1. CYP24A1 mRNA induction in RPTCs was also inhibited by the protein synthesis inhibitor cycloheximide. |
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| 16817231 | Siri S, Chen MJ, Chen TT: Biological activity of rainbow trout Ea4-peptide of the pro-insulin-like growth factor (pro-IGF)-I on promoting attachment of breast cancer cells (MDA-MB-231) via alpha2- and beta1-integrin. J Cell Biochem. 2006 Dec 15;99(6):1524-35. Expression of fibronectin 1 gene induced by rtEa4-peptide in MDA-MB-231 cells was abolished by inhibitors of PI3K, PKC, Mek1/2, JNK1/2, and p38 MAPK signaling transduction molecules. Blocking new protein synthesis by cycloheximide significantly reduced the attachment of MDA-MB-231 cells to rtEa4-peptide coated wells by 50%. |
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| 18086809 | Stone MK, Kolling GL, Lindner MH, Obrig TG: p38 mitogen-activated protein kinase mediates lipopolysaccharide and tumor necrosis factor alpha induction of shiga toxin 2 sensitivity in human umbilical vein endothelial cells. Infect Immun. 2008 Mar;76(3):1115-21. Epub 2007 Dec 17. Cell viability assays indicated that the p38 mitogen-activated protein kinase (MAPK) inhibitors SB202190 and SB203580 and the general protein synthesis inhibitor cycloheximide inhibited both the LPS and TNF-alpha sensitization of HUVEC to Stx2, while all other inhibitors tested did not inhibit this sensitization. In conclusion, our results show that LPS and TNF-alpha induction of Stx2 sensitivity in HUVEC is mediated through a pathway that includes p38 MAPK. |
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| 15647476 | Verma P, Chierzi S, Codd AM, Campbell DS, Meyer RL, Holt CE, Fawcett JW: Axonal protein synthesis and degradation are necessary for efficient growth cone regeneration. J Neurosci. 2005 Jan 12;25(2):331-42. Application of protein synthesis inhibitors (cycloheximide and anisomycin) to cut axons, including axons whose cell bodies were removed, or proteasome inhibitors (lactacystin and N-acetyl-Nor---Al) all result in a reduction in the proportion of transected axons able to reform growth cones. Similar inhibition of growth cone formation was observed on addition of target of rapamycin (TOR), p38 MAPK (mitogen-activated protein kinase), and caspase-3 inhibitors. |
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| 17308068 | Bachmann M, Dragoi C, Poleganov MA, Pfeilschifter J, Muhl H: Interleukin-18 directly activates T-bet expression and function via p38 mitogen-activated protein kinase and nuclear factor-kappaB in acute myeloid leukemia-derived predendritic KG-1 cells. Mol Cancer Ther. 2007 Feb;6(2):723-31. Blockage of translation by cycloheximide, usage of neutralizing antibodies, and the inability of IFNgamma to mediate significant p38 mitogen-activated protein kinase activation in KG-1 cells clearly revealed that activation of T-bet was not via autocrine IFNgamma. |
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| 19683494 | Descot A, Hoffmann R, Shaposhnikov D, Reschke M, Ullrich A, Posern G: Negative regulation of the EGFR-MAPK cascade by actin-MAL-mediated Mig6/Errfi-1 induction. Mol Cell. 2009 Aug 14;35(3):291-304. Upregulation of Mig6 by lipid agonists such as LPA and S1P or actin drugs involved MAL and correlated with decreased activation of EGFR, MAPK/Erk, and c-fos. |
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| 17615150 | Lamirand A, Mercier G, Ramauge M, Pierre M, Courtin F: Hypoxia stabilizes type 2 deiodinase activity in rat astrocytes. . Endocrinology. 2007 Oct;148(10):4745-53. Epub 2007 Jul 5. Specific inhibitors of ERK, p38 MAPK, or phosphatidylinositol 3-kinase pathways were without any effect on hypoxia-increased D2 activity, eliminating their role in the effects of hypoxia. Cycloheximide did not block the effect of hypoxia on D2 activity and D2 half-life was enhanced under hypoxia demonstrating a posttranslational action of hypoxia. |
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| 16684952 | Cohen M, Marchand-Adam S, Lecon-Malas V, Marchal-Somme J, Boutten A, Durand G, Crestani B, Dehoux M: HGF synthesis in human lung fibroblasts is regulated by oncostatin M. . Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1097-103. OSM-induced HGF secretion was inhibited by PD-98059 (a specific pharmacological inhibitor of ERK1/2), SB-203580 (a p38 MAPK inhibitor), and SP-600125 (a JNK inhibitor) by 70, 82, and 100%, respectively, whereas basal HGF secretion was only inhibited by SP-600125 by 30%. HGF was released in its cleaved mature form, and its secretion was completely inhibited in the presence of cycloheximide, indicating a de novo protein synthesis. |
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| 18493739 | Wang Q, Heimberg H, Pipeleers D, Ling Z: Glibenclamide activates translation in rat pancreatic beta cells through -dependent mTOR, PKA and MEK signalling pathways. Diabetologia. 2008 Jul;51(7):1202-12. Epub 2008 May 21. Since it was blocked by the translation inhibitor cycloheximide, we examined whether sustained exposure to glibenclamide activates translational factors by -dependent signalling pathways. This activation of translational factors and the associated elevation of insulin synthesis were completely blocked by the calcium channel blocker and partially blocked by the mammalian target of rapamycin (mTOR) inhibitor rapamycin, the protein kinase A (PKA) inhibitor Rp-8-Br-cAMPs and the mitogen-activated protein kinase/ extracellular signal-regulated kinase kinase (MEK) inhibitor U0126; a combination of inhibitors exhibited additive effects. |
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| 15585589 | Chen YH, Hung PF, Kao YH: IGF-I downregulates resistin gene expression and protein secretion. . Am J Physiol Endocrinol Metab. 2005 May;288(5):E1019-27. Epub 2004 Dec 7. These data demonstrate that IGF-I downregulates Rstn gene expression via IGF-IR-dependent and MEK1-, p38 MAPK-, and phosphoinositide 3-kinase-independent pathways and likely modifies the distribution of Rstn protein between the intracellular and extracellular compartments via a p38 MAPK-dependent pathway. Treatment with cycloheximide, but not with actinomycin D, prevented IGF-I-suppressed Rstn mRNA expression, suggesting that IGF-I destabilizes Rstn mRNA and that IGF-I's effect requires new protein, but not mRNA, synthesis. |
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| 15519494 | Yoshida M, Niwa M, Ishisaki A, Hirade K, Ito H, Shimizu K, Kato K, Kozawa O: Methotrexate enhances -stimulated heat shock protein 27 induction in osteoblasts. Prostaglandins Leukot Essent Fatty Acids. 2004 Dec;71(6):351-62. We previously showed that stimulates the induction of heat shock protein 27 (HSP27) via protein kinase C (PKC)-dependent p38 mitogen-activated protein (MAP) kinase and p44/p42 MAP kinase in osteoblast-like MC3T3-E1 cells. |
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| 17257889 | Nakagawa H, Matsumiya T, Sakaki H, Imaizumi T, Kubota K, Kusumi A, Kobayashi W, Kimura H: Expression of vascular endothelial growth factor by photodynamic therapy with mono-L-aspartyl chlorin e6 (NPe6) in oral squamous cell carcinoma. Oral Oncol. 2007 Jul;43(6):544-50. Epub 2007 Jan 25. The expression mRNAs for VEGF, c-jun and c-fos induced by Npe6-mediated PDT were inhibited by SB203580, p38 MAPK inhibitors, and the expression of VEGF mRNA was inhibited by cycloheximide (CHX), a protein synthesis inhibitor. We conclude that Npe6-mediated PDT induces the expression of VEGF, c-jun and c-fos in human oral carcinoma cell line, HSC-3 cell, and at least partly, through the activation of p38 MAPK. |
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| 16787641 | Ito Y, Oh-Hashi K, Kiuchi K, Hirata Y: p44/42 MAP kinase and c-Jun N-terminal kinase contribute to the up-regulation of caspase-3 in -induced apoptosis in PC12 cells. Brain Res. 2006 Jul 12;1099(1):1-7. Epub 2006 Jun 19. Up-regulation of caspase-3 protein was evident in -treated PC12 cells and was moderate in cisplatin-, rotenone- and A23187-treated cells but was not observed in serum deprivation-, anisomycin-, camptothecin-, cycloheximide- or staurosporine-treated cells in which all treatments induced extensive DNA fragmentation. These results suggest that p44/42 MAPK contributes to the up-regulation of caspase-3 mRNA and the JNK pathway regulates caspase-3 protein levels posttranslationally in -induced apoptosis in PC12 cells. |
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| 17433310 | Ortiz-Masia D, Perez-Amador MA, Carbonell J, Marcote MJ: Diverse stress signals activate the C1 subgroup MAP kinases of Arabidopsis. FEBS Lett. 2007 May 1;581(9):1834-40. Epub 2007 Apr 9. Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in plants. An increase in their kinase activity was detected in response to wounding that was blocked by cycloheximide. |
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| 16912191 | Ma Y, Yu WD, Kong RX, Trump DL, Johnson CS: Role of nongenomic activation of phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase/extracellular signal-regulated kinase kinase/extracellular signal-regulated kinase 1/2 pathways in 1,25D3-mediated apoptosis in squamous cell carcinoma cells. Cancer Res. 2006 Aug 15;66(16):8131-8. These effects were nongenomic: they occurred rapidly and were not inhibited by cycloheximide or actinomycin D. |
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| 17113042 | Hsu YL, Chang JK, Tsai CH, Chien TT, Kuo PL: induces human osteoblast differentiation through bone morphogenetic protein-2/p38 mitogen-activated protein kinase pathway. Biochem Pharmacol. 2007 Feb 15;73(4):504-14. Epub 2006 Oct 26. Induction of differentiation by is associated with increased activation of SMAD1/5/8 and p38 mitogen-activated protein kinases. |
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| 19463893 | Claasen AM, Guevremont D, Mason-Parker SE, Bourne K, Tate WP, Abraham WC, Williams JM: Secreted amyloid precursor protein-alpha upregulates synaptic protein synthesis by a protein kinase G-dependent mechanism. Neurosci Lett. 2009 Aug 21;460(1):92-6. Epub 2009 May 20. This was dose-dependent and blocked completely by inhibitors of protein synthesis (cycloheximide) and of -dependent protein kinase (KT5823). Inhibitors of /calmodulin-dependent protein kinases (KN62) and mitogen-activated protein kinase (PD98059) partially blocked the response. |
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| 19233135 | Kato T, Ikemoto M, Hato F, Kitagawa S: Granule swelling and cleavage of mitogen-activated protein kinases in human neutrophils undergoing apoptosis. Biochem Biophys Res Commun. 2009 Apr 10;381(3):434-8. Epub 2009 Feb 20. Extracellular signal-regulated kinase and p38 have been shown to be cleaved in human neutrophils undergoing apoptosis induced by tumor necrosis factor-alpha and cycloheximide. |
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| 17571317 | Peng ML, Huang HF, Jin F: [Progress in research on oocytes parthenogenetic activation] . Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007 May;36(3):307-12. The activation of oocyte is the result of oscillations and deactivation of some cytokines such as maturation promoting factor, mitogen-activated protein kinase and cytostatic factor. |
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| 15743925 | Phagoo SB, Reddi K, Silvallana BJ, Leeb-Lundberg LM, Warburton D: Infection-induced kinin B1 receptors in human pulmonary fibroblasts: role of intact pathogens and p38 mitogen-activated protein kinase-dependent signaling. J Pharmacol Exp Ther. 2005 Jun;313(3):1231-8. Epub 2005 Mar 2. The protein synthesis inhibitor cycloheximide and transcriptional inhibitor actinomycin D abrogated the B (1) response to B. cen. indicating de novo B (1) R synthesis. |
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| 17498698 | Ohnishi M, Katsuki H, Fujimoto S, Takagi M, Kume T, Akaike A: Involvement of thrombin and mitogen-activated protein kinase pathways in hemorrhagic brain injury. Exp Neurol. 2007 Jul;206(1):43-52. Epub 2007 Apr 14. Intracerebroventricular injection of argatroban or cycloheximide, both of which prevent thrombin cytotoxicity in vitro, exhibited a significant neuroprotective effect against ICH-induced injury. |
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| 16978838 | Hu JH, Chen T, Zhuang ZH, Kong L, Yu MC, Liu Y, Zang JW, Ge BX: Feedback control of MKP-1 expression by p38. . Cell Signal. 2007 Feb;19(2):393-400. Epub 2006 Jul 25. Mitogen-activated protein (MAP) kinases play a critical role in innate immune responses to microbial infection through eliciting the biosynthesis of proinflammatory cytokines. The mRNA level of MKP-1 is not affected by inhibition of p38, but the expression of MKP-1 is inhibited by treatment of cycloheximide. |
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| 16874031 | Finn PF, Mesires NT, Vine M, Dice JF: Effects of small molecules on chaperone-mediated autophagy. Autophagy. 2005 Oct-Dec;1(3):141-5. Epub 2005 Oct 11. CMA is also partially inhibited when the p38 mitogen activated protein kinase is blocked. We demonstrate that CMA, like MA, is inhibited by protein synthesis inhibitors anisomycin and cycloheximide. |
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| 19454725 | Karahashi H, Michelsen KS, Arditi M: Lipopolysaccharide-induced apoptosis in transformed bovine brain endothelial cells and human dermal microvessel endothelial cells: the role of JNK. J Immunol. 2009 Jun 1;182(11):7280-6. Stimulation of transformed bovine brain endothelial cells (TBBEC) with LPS leads to apoptosis while human microvessel endothelial cells (HMEC) need the presence of cycloheximide (CHX) with LPS to induce apoptosis. To investigate the molecular mechanism of LPS-induced apoptosis in HMEC or TBBEC, we analyzed the involvement of MAPK and PI3K in TBBEC and HMEC. |
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| 16339571 | Han B, Mura M, Andrade CF, Okutani D, Lodyga M, dos Santos CC, Keshavjee S, Matthay M, Liu M: TNFalpha-induced long pentraxin PTX3 expression in human lung epithelial cells via JNK. J Immunol. 2005 Dec 15;175(12):8303-11. Pretreatment with either actinomycin D or cycloheximide abolished TNF-alpha-induced PTX3 expression, indicating the requirement for both transcriptional and translational regulation. |
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| 16123165 | Ishikawa T, Hwang K, Lazzarino D, Morris PL: Sertoli cell expression of steroidogenic acute regulatory protein-related lipid transfer 1 and 5 domain-containing proteins and sterol regulatory element binding protein-1 are interleukin-1beta regulated by activation of c-Jun N-terminal kinase and cyclooxygenase-2 and cytokine induction. Endocrinology. 2005 Dec;146(12):5100-11. Epub 2005 Aug 25. IL-1beta rapidly decreases levels of precursor and mature sterol regulatory element-binding protein-1, changes not altered by cycloheximide, suggesting coordinate regulation of StARD1 and -D5, but not StARD4, expression. |
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| 16099857 | Terriff DL, Chik CL, Price DM, Ho AK: Proteasomal proteolysis in the adrenergic induction of arylalkylamine-N-acetyltransferase in rat pinealocytes. Endocrinology. 2005 Nov;146(11):4795-803. Epub 2005 Aug 11. This possibility was also supported by the finding that the inhibitory effect of c-lact on NE-induced aa-nat induction was markedly reduced by cycloheximide, a protein synthesis inhibitor. |
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| 16951370 | Rahman MS, Yamasaki A, Yang J, Shan L, Halayko AJ, Gounni AS: IL-17A induces eotaxin-1/CC chemokine ligand 11 expression in human airway smooth muscle cells: role of MAPK (Erk1/2, JNK, and p38) pathways. J Immunol. 2006 Sep 15;177(6):4064-71. Moreover, IL-17A significantly induced eotaxin-1/CCL11 release and mRNA expression, an effect that was abrogated with cycloheximide and actinomycin D treatment. |
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| 18729634 | McGauran AM, Moore JB, Madsen D, Barry D, O'Dea S, Mahon BP, Commins S: A possible role for protein synthesis, extracellular signal-regulated kinase, and brain-derived neurotrophic factor in long-term spatial memory retention in the water maze. Behav Neurosci. 2008 Aug;122(4):805-15. Both brain-derived neurotrophic factor (BDNF) and extracellular signal-regulated kinase (ERK) are known to play an important role in protein synthesis-dependent memory consolidation, via the mitogen-activated protein-kinase (MAP-K) signaling pathway during the transcription phase of protein synthesis. The current study investigates the influence of protein synthesis inhibition (PSI) by cycloheximide on spatial learning and memory. |
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| 16282198 | Chao JT, Martinez-Lemus LA, Kaufman SJ, Meininger GA, Ramos KS, Wilson E: Modulation of alpha7-integrin-mediated adhesion and expression by platelet-derived growth factor in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2006 Apr;290(4):C972-80. Epub 2005 Nov 9. Actinomycin D and cycloheximide attenuated PDGF-induced increases in alpha (7)-integrin, indicating the involvement of de novo mRNA and protein synthesis. Treatment with the MAPK inhibitors PD-98059, SP-600125, and SB-203580 attenuated PDGF-induced increases in mRNA. |
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| 17989348 | Papineni S, Chintharlapalli S, Safe S: Methyl 2-cyano-3,11-dioxo-18 beta-olean-1,12-dien-30-oate is a peroxisome proliferator-activated receptor-gamma agonist that induces receptor-independent apoptosis in LNCaP prostate cancer cells. Mol Pharmacol. 2008 Feb;73(2):553-65. Epub 2007 Nov 7. However, induction of these responses by beta-CDODA-Me was PPARgamma-independent and due to activation of phosphatidylinositol-3-kinase, mitogen-activated protein kinase, and jun N-terminal kinase pathways by this compound. In contrast, beta-CDODA-Me also decreased androgen receptor (AR) and prostate-specific antigen (PSA) mRNA and protein levels through kinase-independent pathways. beta-CDODA-Me repressed AR mRNA transcription, whereas decreased PSA mRNA levels were dependent on protein synthesis and were reversed by cycloheximide. |
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| 16467189 | El Sayah M, Medeiros R, Fernandes ES, Campos MM, Calixto JB: Mechanisms underlying lipopolysaccharide-induced kinin B1 receptor up-regulation in the pig iris sphincter in vitro. Mol Pharmacol. 2006 May;69(5):1701-8. Epub 2006 Feb 7. The up-regulation of B1 receptors by lipopolysaccharide stimulation was decreased by the inhibitors of protein synthesis, cycloheximide and actinomycin D, and by dexamethasone and the nuclear factor-kappaB (NF-kappaB) inhibitor pyrrolidinedithiocarbamate (PDTC). Molecular biology experiments demonstrated that in vitro incubation with lipopolysaccharide resulted in a time-dependent and remarkable activation of NF-kappaB and of p38 and ERK1/2 mitogen-activated protein (MAP) kinases, in pig iris sphincter preparations. |
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| 16034134 | Grehan JF, Levay-Young BK, Fogelson JL, Francois-Bongarcon V, Benson BA, Dalmasso AP: IL-4 and IL-13 induce protection of porcine endothelial cells from killing by human complement and from apoptosis through activation of a phosphatidylinositide 3-kinase/Akt pathway. J Immunol. 2005 Aug 1;175(3):1903-10. We found that porcine EC incubated with IL-4 or IL-13, but not with IL-10 or IL-11, became protected from killing by complement and apoptosis induced by TNF-alpha plus cycloheximide. |
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| 18303854 | Zanatta L, Rosso A, Folador P, Figueiredo MS, Pizzolatti MG, Leite LD, Silva FR: Insulinomimetic effect of 3-neohesperidoside on the rat soleus muscle. J Nat Prod. 2008 Apr;71(4):532-5. Epub 2008 Feb 28. However, no significant change occurred on uptake stimulated by 1 when muscles were pretreated with PD98059, an inhibitor of mitogen-activated protein kinase (MEK), and cycloheximide, an inhibitor of protein synthesis. |
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| 16236272 | Ho AK, McNeil L, Terriff D, Price DM, Chik CL: Role of protein turnover in the activation of p38 mitogen-activated protein kinase in rat pinealocytes. Biochem Pharmacol. 2005 Dec 5;70(12):1840-50. Epub 2005 Oct 19. The stimulatory effect of NE on phosphorylated MKK3/6 and p38MAPK, but not phosphorylated p42/44MAPK, was blocked by treatment with actinomycin or cycloheximide, indicating a requirement of transcription and translation in activation of the p38MAPK but not the p42/44MAPK pathway. |
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| 16492674 | Berry FB, Mirzayans F, Walter MA: Regulation of FOXC1 stability and transcriptional activity by an epidermal growth factor-activated mitogen-activated protein kinase signaling cascade. J Biol Chem. 2006 Apr 14;281(15):10098-104. Epub 2006 Feb 21. |
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| 16197501 | Ghosh M, Gharami K, Paul S, Das S: Thyroid hormone-induced morphological differentiation and maturation of astrocytes involves activation of protein kinase A and ERK signalling pathway. Eur J Neurosci. 2005 Oct;22(7):1609-17. Further studies showed that TH treatment resulted in a biphasic response on the cellular phospho-MAP kinase (p-MAPK or p-ERK) level: an initial decline in the p-ERK level followed by an induction at 18-24 h, both of which could be blocked by a PKA inhibitor. |
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| 17158707 | Bogoyevitch MA, Kobe B: Uses for JNK: the many and varied substrates of the c-Jun N-terminal kinases. Microbiol Mol Biol Rev. 2006 Dec;70(4):1061-95. The c-Jun N-terminal kinases (JNKs) are members of a larger group of serine/ (Ser/ protein kinases from the mitogen-activated protein kinase family. JNKs were originally identified as stress-activated protein kinases in the livers of cycloheximide-challenged rats. |
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| 15788394 | Hansen IA, Attardo GM, Roy SG, Raikhel AS: Target of rapamycin-dependent activation of S6 kinase is a central step in the transduction of nutritional signals during egg development in a mosquito. J Biol Chem. 2005 May 27;280(21):20565-72. Epub 2005 Mar 23. This increase is sensitive to the TOR inhibitor rapamycin in a concentration-dependent manner but not to the phosphatidylinositol 3-kinase/phosphatidylinositol 3-kinase-related kinase inhibitor LY294002, the MAPK inhibitor PD98059, or the translational inhibitor cycloheximide. |
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| 17209135 | Kuang PP, Zhang XH, Rich CB, Foster JA, Subramanian M, Goldstein RH: Activation of elastin transcription by transforming growth factor-beta in human lung fibroblasts. Am J Physiol Lung Cell Mol Physiol. 2007 Apr;292(4):L944-52. Epub 2007 Jan 5. The induction of elastin hnRNA and mRNA expression by TGF-beta was abolished by pretreatments with TGF-beta receptor I inhibitor, global transcription inhibitor actinomycin D, and partially blocked by addition of protein synthesis inhibitor cycloheximide, but was not affected by the p44/42 MAPK inhibitor U0126. |
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| 18339016 | Fujisawa T, Ide K, Holtzman MJ, Suda T, Suzuki K, Kuroishi S, Chida K, Nakamura H: Involvement of the p38 MAPK pathway in IL-13-induced mucous cell metaplasia in mouse tracheal epithelial cells. Respirology. 2008 Mar;13(2):191-202. Cycloheximide also diminished activation of p38 MAPK and induction of MUC5AC mRNA expression by IL-13. |
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| 16433893 | Cao YX, Xu CB, Luo GG, Edvinsson L: Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways. Basic Clin Pharmacol Toxicol. 2006 Jan;98(1):61-7. Transcriptional inhibitor actinomycin D, translational inhibitor cycloheximide, protein kinase C inhibitors (staurosporine and RO31-8220) and mitogen-activated protein kinase (MAPK) pathway inhibitors (SB386023, U0126 and SB239063) prevented the increase in NA-induced contractions. |
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| 19396617 | Carraro-Lacroix LR, Girardi AC, Malnic G: Long-term regulation of vacuolar H (+)-ATPase by angiotensin II in proximal tubule cells. Pflugers Arch. 2009 Sep;458(5):969-79. Epub 2009 Apr 26. Inhibition of phosphatidylinositol-3-kinase (PI3K) by wortmannin and of p38 mitogen-activated protein kinase (MAPK) by SB 203580 also blocked this effect. |
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| 19286921 | Croons V, Martinet W, Herman AG, Timmermans JP, De Meyer GR: The protein synthesis inhibitor anisomycin induces macrophage apoptosis in rabbit atherosclerotic plaques through p38 mitogen-activated protein kinase. J Pharmacol Exp Ther. 2009 Jun;329(3):856-64. Epub 2009 Mar 13. We showed recently that the protein synthesis inhibitor cycloheximide, in contrast to puromycin, selectively depleted macrophages in rabbit atherosclerotic plaques without affecting smooth muscle cells (SMCs). |
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| 15640153 | Pillinger MH, Marjanovic N, Kim SY, Scher JU, Izmirly P, Tolani S, Dinsell V, Lee YC, Blaser MJ, Abramson SB: Matrix metalloproteinase secretion by gastric epithelial cells is regulated by E prostaglandins and MAPKs. J Biol Chem. 2005 Mar 18;280(11):9973-9. Epub 2005 Jan 7. MMP-1 secretion required activation of the MAPK Erk and subsequent protein synthesis but was down-regulated by the alternate MAPK, p38. |
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| 19664126 | Misiak-Tloczek A, Brzezinska-Blaszczyk E: IL-6, but not IL-4, stimulates chemokinesis and TNF stimulates chemotaxis of tissue mast cells: involvement of both mitogen-activated protein kinases and phosphatidylinositol 3-kinase signalling pathways. APMIS. 2009 Aug;117(8):558-67. We also documented that the migration response of mast cells to stimulation with IL-6 and TNF was mediated through signal transduction pathways involving mitogen-activated protein kinases and phosphatidylinositol 3-kinase. |
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| 20339915 | Ji L, Chauhan A, Chauhan V: Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells. Neurochem Res. 2010 Mar 26. -mediated up-regulation of c-gelsolin was inhibited by cycloheximide (a general inhibitor of protein synthesis). |
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| 16881805 | Nervina JM, Camargo PM, Bezouglaia O, Tetradis S: Prostanoid- and interleukin-1-induced primary genes in cementoblastic cells. J Periodontol. 2006 Aug;77(8):1362-70. METHODS: Confluent OCCM cells were pretreated with the protein synthesis inhibitor cycloheximide followed by fluprostenol or IL-1 for 1.5 hours. cDNA generated from each group was used for cDNA subtraction hybridization to identify differentially induced genes. |
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| 18706402 | Sadler SE, Archer MR, Spellman KM: Activation of the -signaling pathway by methyl-beta-cyclodextrin or steroid in Xenopus laevis oocytes involves release of 45-kDa Galphas. Dev Biol. 2008 Oct 1;322(1):199-207. Epub 2008 Jul 31. The protein synthesis inhibitor, cycloheximide, blocked the appearance of Mos, blocked MebetaCD-stimulated phosphorylation of MAPK, and inhibited MebetaCD-induced oocyte maturation. |
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| 16283438 | Yao Y, Kubota T, Sato K, Takeuchi H, Handa Y, Matsukawa S: Cell-specific but p53-independent regulation of vascular endothelial growth factor expression by interferons in human glioblastoma cells. J Neurooncol. 2006 Feb;76(3):219-25. Interestingly, cycloheximide also blocked IFN-induced activation of the p44/p42 mitogen-activated protein kinase, which was partially required for induction of VEGF by IFNs. |
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| 15633612 | Yang S, Yip R, Polena S, Sharma M, Rao S, Griciene P, Gintautas J, Jerome H: Reactive species increased focal adhesion kinase production in pulmonary microvascular endothelial cells. Proc West Pharmacol Soc. 2004;47:54-6. Mitogen-activated protein (MAP) kinases are a group of 30- to 110-kDa serine/threonine kinases. We found that low dose H2O2 induced increased FAK production in pulmonary microvascular endothelial cells, which could be blocked by cycloheximide (CHX), a protein synthesis inhibitor. |
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| 16753025 | Li CF, Hughes-Fulford M: Fibroblast growth factor-2 is an immediate-early gene induced by mechanical stress in osteogenic cells. J Bone Miner Res. 2006 Jun;21(6):946-55. Pretreatment of MC3T3-E1 cells with cycloheximide showed that the induction of fgf-2 did not require new protein synthesis. Pretreating MC3T3-E1 cells with the mitogen-activated protein kinase (MAPK)/ERK kinase 1/2 (MEK1/2) inhibitor, U0126, or H-89, a PKA inhibitor, significantly inhibited the induction of fgf-2, showing that mechanical induction of fgf-2 is dependent on ERK and PKA signaling pathways. |
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| 15525600 | Lin KH, Chen CY, Chen SL, Yen CC, Huang YH, Shih CH, Shen JJ, Yang RC, Wang CS: Regulation of fibronectin by thyroid hormone receptors. . J Mol Endocrinol. 2004 Oct;33(2):445-58. Blockade of protein synthesis by cycloheximide almost completely inhibited the concomitant induction of FN mRNA by T3, indicating that T3 indirectly regulates FN. In an effort to elucidate the we demonstrated the involvement of the signaling pathways involved in the activation of FN by T3, mitogen activated protein kinase/c-Jun N-terminal kinase/p38 MAPK (MAPK/JNK/p38) pathway. |
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| 16198345 | Lim WC, Park M, Bahn JJ, Inoue H, Lee YJ: Hypertonic induction of cyclooxygenase-2 occurs independently of NF-kappaB and is inhibited by the glucocorticoid receptor in A549 cells. FEBS Lett. 2005 Oct 10;579(24):5430-6. Hypertonicity modulates prostaglandin synthesis by influencing cyclooxygenase-2 (COX-2) expression in tissues such as liver and kidney via a mitogen-activated protein kinase (MAPK)-dependent pathway. This induction was a transcriptional event that occurred in the absence of the protein synthesis inhibitor cycloheximide and was the result of enhanced promoter activity, as examined with the use of full-length COX-2 promoter-driven reporter plasmids. |
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| 17182689 | Mangino G, Percario ZA, Fiorucci G, Vaccari G, Manrique S, Romeo G, Federico M, Geyer M, Affabris E: In vitro treatment of human monocytes/macrophages with myristoylated recombinant Nef of human immunodeficiency virus type 1 leads to the activation of mitogen-activated protein kinases, IkappaB kinases, and interferon regulatory factor 3 and to the release of beta interferon. J Virol. 2007 Mar;81(6):2777-91. Epub 2006 Dec 20. Previously, we reported that Nef treatment of primary human monocyte-derived macrophages (MDMs) induces a cycloheximide-independent activation of NF-kappaB and the synthesis and secretion of a set of chemokines/cytokines that activate STAT1 and STAT3. |
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| 18223264 | Molloy ES, Morgan MP, Doherty GA, McDonnell B, O'Byrne J, Fitzgerald DJ, McCarthy GM: Mechanism of basic crystal-stimulated matrix metalloproteinase-13 expression by osteoarthritic synovial fibroblasts: inhibition by Ann Rheum Dis. 2008 Dec;67(12):1773-9. Epub 2008 Jan 26. BCP crystal-stimulated MMP-13 mRNA expression was blocked by inhibition of the extracellular regulated kinase (ERK1/2) and p38 mitogen activated protein kinase (MAPK) pathways and inhibition of the activation of nuclear factor kappaB. |
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| 19716839 | Charlson AT, Zeliadt NA, Wattenberg EV: Extracellular signal regulated kinase 5 mediates signals triggered by the novel tumor promoter palytoxin. Toxicol Appl Pharmacol. 2009 Dec 1;241(2):143-53. Epub 2009 Aug 28. Cycloheximide, okadaic acid, and orthovanadate did not mimic the effect of palytoxin on ERK5. We previously showed that palytoxin activates three major members of the mitogen activated protein kinase (MAPK) family, extracellular signal regulated kinase 1 and 2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38. |
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| 16141313 | Tian Q, Li J, Xie X, Sun M, Sang H, Zhou C, An T, Hu L, Ye RD, Wang MW: Stereospecific induction of nuclear factor-kappaB activation by isochamaejasmin. Mol Pharmacol. 2005 Dec;68(6):1534-42. Epub 2005 Sep 1. Isochamaejasmin also induced time-dependent phosphorylation of the mitogen-activated protein kinases extracellular signal-regulated kinase 1/2 and p38, and a novel protein kinase C (PKCdelta). It is noteworthy that the two stereoisomers and the methyl derivative did not induce detectable activation of NF-kappaB and were more cytotoxic than isochamaejasmin, which could partially rescue cycloheximide-induced apoptosis. |
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| 19633059 | Motizuki M, Xu Z: Importance of polarisome proteins in reorganization of actin cytoskeleton at low pH in Saccharomyces cerevisiae. J Biochem. 2009 Nov;146(5):705-12. Epub 2009 Jul 24. We reported previously that S. cerevisiae responds to low-pH stress by transiently depolarizing its actin cytoskeleton, and that this step requires a mitogen-activated protein kinase, high osmolarity 1 (Hog1p). The protein-synthesis inhibitor cycloheximide did not influence the time course of actin depolarization, suggesting that the depolarization is a direct effect of the HOG pathway. |
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| 17177854 | Neu R, Adams S, Munz B: Differential expression of entactin-1/nidogen-1 and entactin-2/nidogen-2 in myogenic differentiation. Differentiation. 2006 Dec;74(9-10):573-82. Furthermore, treatment with the translation inhibitor cycloheximide could show that entactin-2 is a primary response gene. |
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| 16495213 | Carraro-Lacroix LR, Ramirez MA, Zorn TM, Reboucas NA, Malnic G: Increased NHE1 expression is associated with serum deprivation-induced differentiation in immortalized rat proximal tubule cells. Am J Physiol Renal Physiol. 2006 Jul;291(1):F129-39. Epub 2006 Feb 21. The altered activity of NHE1 was consistent with an increase of both transcription and translation of the antiporter, as the utilization of actinomycin D and cycloheximide significantly inhibited the upregulation of NHE1 induced by serum withdrawal. |
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