| Name | PPAR gamma |
|---|---|
| Synonyms | HUMPPARG; PAX8/PPARG fusion gene; NR1C3; PPAR gamma; PPAR gamma2; PPARG; PPARG 1; PPARG 2… |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 18497737 | He F, Doucet JA, Stephens JM: Caspase-mediated degradation of PPARgamma proteins in adipocytes. . Obesity (Silver Spring). 2008 Aug;16(8):1735-41. Epub 2008 May 22. RESULTS: We observed that TNFalpha can induce a caspase-mediated degradation of PPARgamma proteins in the presence of cycloheximide. |
119(1,2,2,9) | Details |
| 16388013 | Banerjee J, Komar CM: Effects of luteinizing hormone on peroxisome proliferator-activated receptor gamma in the rat ovary before and after the gonadotropin surge. Reproduction. 2006 Jan;131(1):93-101. Cells were cultured with LH in the absence and presence of H89 and cycloheximide to investigate the role of PKA and protein synthesis in the LH-mediated decline in mRNA for PPARgamma respectively. |
95(1,1,2,10) | Details |
| 16982041 | Wang ZJ, Liang CL, Li GM, Yu CY, Yin M: Neuroprotective effects of against oxidative stress on rat hippocampal slices. Chem Biol Interact. 2006 Nov 7;163(3):207-17. Epub 2006 Aug 18. MK886 (5 microM; a noncompetitive inhibitor of proliferator-activated receptor [PPAR] alpha), BADGE (bisphenol A diglycidyl ether; 100 microM; an antagonist of PPARgamma) and cycloheximide (CHX; 30 microM; an inhibitor of protein synthesis) were tested for their effects on the neuroprotection afforded by |
82(1,1,1,2) | Details |
| 17622750 | Sommer M, Wolf G: increases PPARgamma in renal tubular epithelial cells and protects against damage by peroxide. Am J Nephrol. 2007;27(4):425-34. Epub 2007 Jul 3. This increase in PPARgamma protein content was almost totally abolished in the presence of 1 microg/ml cycloheximide, but was only marginally reduced by 0.1 microg/ml actinomycin D. |
46(0,1,2,11) | Details |
| 17089002 | Fiatte C, Huin C, Bertin I, Lesuffleur T, Pluvinet A, Touche N, Plenat F, Dauca M, Domenjoud L, Schohn H: Genetic analysis of peroxisome proliferator-activated receptor gamma1 splice variants in human colorectal cell lines. Int J Oncol. 2006 Dec;29(6):1601-10. HT-29 cells treatment with actinomycin D or cycloheximide showed that the presence of PPARgamma mRNA including exon A2 was dependent on de novo protein synthesis. |
12(0,0,1,7) | Details |
| 18422969 | Necela BM, Su W, Thompson EA: Toll-like receptor 4 mediates cross-talk between peroxisome proliferator-activated receptor gamma and nuclear factor-kappaB in macrophages. Immunology. 2008 Nov;125(3):344-58. Epub 2008 Apr 18. Inhibition of PPARgamma expression was not blocked by cycloheximide, indicating that de novo protein synthesis is not required for LPS-mediated suppression of PPARgamma. |
7(0,0,0,7) | Details |
| 17848638 | Kershaw EE, Schupp M, Guan HP, Gardner NP, Lazar MA, Flier JS: PPARgamma regulates adipose triglyceride lipase in adipocytes in vitro and in vivo. Am J Physiol Endocrinol Metab. 2007 Dec;293(6):E1736-45. Epub 2007 Sep 11. -mediated induction of ATGL mRNA is rapid and is not inhibited by the protein synthesis inhibitor cycloheximide, indicating that intervening protein synthesis is not required for this effect. |
6(0,0,0,6) | Details |
| 15939809 | Clasen R, Schupp M, Foryst-Ludwig A, Sprang C, Clemenz M, Krikov M, Thone-Reineke C, Unger T, Kintscher U: PPARgamma-activating angiotensin type-1 receptor blockers induce adiponectin. Hypertension. 2005 Jul;46(1):137-43. Epub 2005 Jun 6. Kinetic studies using the protein synthesis inhibitor cycloheximide showed that irbesartan prevented the cellular depletion of adiponectin protein. |
4(0,0,0,4) | Details |
| 19118520 | Yamauchi A, Takahashi I, Takasawa S, Nata K, Noguchi N, Ikeda T, Yoshikawa T, Shervani NJ, Suzuki I, Uruno A, Unno M, Okamoto H, Sugawara A: Thiazolidinediones inhibit REG Ialpha gene transcription in gastrointestinal cancer cells. Biochem Biophys Res Commun. 2009 Feb 13;379(3):743-8. Epub 2008 Dec 30. We here demonstrated that PPARgamma-agonist thiazolidinediones (TZDs) inhibited cell proliferation and REG Ialpha protein/mRNA expression in gastrointestinal cancer cells. The TZD-induced REG Ialpha mRNA reduction was abolished by cycloheximide, indicating the necessity of novel protein (s) synthesis. |
4(0,0,0,4) | Details |
| 16876120 | Chung SS, Choi HH, Cho YM, Lee HK, Park KS: Sp1 mediates repression of the resistin gene by PPARgamma agonists in 3T3-L1 adipocytes. Biochem Biophys Res Commun. 2006 Sep 15;348(1):253-8. Epub 2006 Jul 18. Resistin gene expression was decreased by TZD in fully differentiated 3T3-L1 adipocytes, which was abolished after treatment of cycloheximide (a protein synthesis inhibitor). |
3(0,0,0,3) | Details |
| 15707762 | Anandharajan R, Pathmanathan K, Shankernarayanan NP, Vishwakarma RA, Balakrishnan A: Upregulation of Glut-4 and PPAR gamma by an isoflavone from Pterocarpus marsupium on L6 myotubes: a possible mechanism of action. J Ethnopharmacol. 2005 Feb 28;97(2):253-60. Epub 2005 Jan 13. The inhibitory effect of cycloheximide on Pterocarpus marsupium methanolic extract and Pterocarpus marsupium isoflavone-mediated uptake suggested that new protein synthesis is required for elevated Glut-4 protein expression. |
3(0,0,0,3) | Details |
| 16716914 | Anandharajan R, Jaiganesh S, Shankernarayanan NP, Viswakarma RA, Balakrishnan A: In vitro uptake activity of Aegles marmelos and Syzygium cumini by activation of Glut-4, PI3 kinase and PPARgamma in L6 myotubes. Phytomedicine. 2006 Jun;13(6):434-41. Epub 2005 Nov 2. The inhibitory effect of cycloheximide on A. marmelos- and S. cumini-mediated uptake suggested that new protein synthesis is required for the elevated transport. |
3(0,0,0,3) | Details |
| 17989348 | Papineni S, Chintharlapalli S, Safe S: Methyl 2-cyano-3,11-dioxo-18 beta-olean-1,12-dien-30-oate is a peroxisome proliferator-activated receptor-gamma agonist that induces receptor-independent apoptosis in LNCaP prostate cancer cells. Mol Pharmacol. 2008 Feb;73(2):553-65. Epub 2007 Nov 7. In contrast, beta-CDODA-Me also decreased androgen receptor (AR) and prostate-specific antigen (PSA) mRNA and protein levels through kinase-independent pathways. beta-CDODA-Me repressed AR mRNA transcription, whereas decreased PSA mRNA levels were dependent on protein synthesis and were reversed by cycloheximide. |
3(0,0,0,3) | Details |
| 17726147 | Jorgensen JA, Zadravec D, Jacobsson A: and synergistically induce Elovl3 expression in brown adipocytes. Am J Physiol Endocrinol Metab. 2007 Nov;293(5):E1159-68. Epub 2007 Aug 28. By using primary cultures of brown adipocytes, we show here that the induced Elovl3 gene expression is synergistically regulated by and the peroxisome proliferator-activated receptor (PPAR) gamma ligand |
2(0,0,0,2) | Details |
| 16150827 | Jiang YJ, Kim P, Elias PM, Feingold KR: LXR and PPAR activators stimulate cholesterol sulfotransferase type 2 isoform 1b in human keratinocytes. J Lipid Res. 2005 Dec;46(12):2657-66. Epub 2005 Sep 8. In this study, we assessed the effect of activators of LXR, PPARalpha, PPARbeta/delta, and PPARgamma on SULT2B1b gene expression and enzyme activity in cultured human keratinocytes (CHKs). In addition, cycloheximide almost completely blocks the ciglitazone-induced increase in SULT2B1b mRNA, suggesting that the transcription of SULTB1b mRNA is dependent on new protein synthesis. |
2(0,0,0,2) | Details |
| 15877607 | Kim HY, Kim HK, Kim JR, Kim HS: Upregulation of LPS-induced chemokine KC expression by 15-deoxy-delta12,14- in mouse peritoneal macrophages. Immunol Cell Biol. 2005 Jun;83(3):286-93. 15-Deoxy-Delta (12,14)-prostaglandin J (2) (15d-PGJ (2)) was initially identified as a high affinity natural ligand for the peroxisome proliferator-activated receptor (PPAR)-gamma. |
1(0,0,0,1) | Details |
| 19448671 | Kar R, Singha PK, Venkatachalam MA, Saikumar P: A novel role for MAP1 LC3 in nonautophagic cytoplasmic vacuolation death of cancer cells. Oncogene. 2009 Jul 16;28(28):2556-68. Epub 2009 May 18. Disruption of sulfhydryl homeostasis, which resulted in ER stress, accumulation of ubiquitinated proteins and subsequent ER dilation, contributed to peroxisome proliferator-activated receptor gamma (PPARgamma)-independent cell death by 15d-PGJ2. Cell death induced by 15d-PGJ2 is prevented by cycloheximide and actinomycin D, suggesting a requirement of new protein synthesis for death with cytoplasmic vacuolation. |
1(0,0,0,1) | Details |
| 18562925 | Mersmann J, Tran N, Zacharowski PA, Grotemeyer D, Zacharowski K: is cardioprotective in a murine model of myocardial I/R. Shock. 2008 Jul;30(1):64-8. The peroxisome proliferator-activated receptor gamma (PPAR-gamma) is a regulator of anti-inflammatory genes. In vitro, cells from the rat cardiomyoblast cell line H9c2 (2-1) are protected against oxidative stress by incubation with which can be abrogated by dexamethasone or cycloheximide. |
1(0,0,0,1) | Details |
| 17302992 | Wang ZJ, Liang CL, Li GM, Yu CY, Yin M: protects primary cultured cortical neurons against oxidative stress. Acta Pharmacol Sin. 2007 Mar;28(3):315-26. Endogenous antioxidant enzymes activity [superoxide dismutases (SOD), peroxidase (GSH-Px), and catalase (CAT)] and lipid peroxidation in cultured cortical neurons were evaluated using commercial kits. {3-[1 (p-chlorobenzyl)- 5-(isopropyl)-3-t-butylthiondol-2-yl]-2,2-dimethylpropanoic acid, Na} [MK886; 5 micromol/L; a noncompetitive inhibitor of proliferator-activated receptor (PPAR) alpha], bisphenol A diglycidyl ether (BADGE; 100 micromol/L; an antagonist of PPAR gamma), and cycloheximide (CHX; 30 micromol/L, an inhibitor of protein synthesis) were tested for their effects on the neuroprotection afforded by |
34(0,1,1,4) | Details |
| 20038583 | Guntur KV, Guilherme A, Xue L, Chawla A, Czech MP: Map4k4 negatively regulates peroxisome proliferator-activated receptor (PPAR) gamma protein translation by suppressing the mammalian target of rapamycin (mTOR) signaling pathway in cultured adipocytes. J Biol Chem. 2010 Feb 26;285(9):6595-603. Epub 2009 Dec 28. PPARgamma degradation rates are remarkably rapid as measured in the presence of cycloheximide (t (1/2) = 2 h), but silencing Map4k4 had no effect on PPARgamma degradation. |
7(0,0,0,7) | Details |
| 19116451 | Lee KJ, Ha ES, Kim MK, Lee SH, Suh JS, Lee SH, Park KH, Park JH, Kim DJ, Kang D, Kim BC, Jeoung D, Kim YK, Kim HD, Hahn JH: CD36 signaling inhibits the translation of heat shock protein 70 induced by oxidized low density lipoprotein through activation of peroxisome proliferators-activated receptor gamma. Exp Mol Med. 2008 Dec 31;40(6):658-68. In this study, we investigated the role of CD36, an OxLDL receptor, and peroxisome proliferator-activated receptor gamma (PPARgamma) in OxLDL-induced Hsp70 expression. Cycloheximide studies demonstrate that troglitazone attenuates Hsp70 translation but not Hsp70 protein stability. |
6(0,0,0,6) | Details |
| 16000871 | Kim HJ, Rho YH, Choi SJ, Lee YH, Cheon H, Um JW, Sohn J, Song GG, Ji JD: 15-Deoxy-delta12,14-PGJ2 inhibits IL-6-induced Stat3 phosphorylation in lymphocytes. Exp Mol Med. 2005 Jun 30;37(3):179-85. 15-deoxy-delta (12,14)-PGJ (2)(15d-PGJ (2)) is a natural ligand that activates the peroxisome proliferators-activated receptor (PPAR) gamma, a member of nuclear receptor family implicated in regulation of lipid metabolism and adipocyte differentiation. Although cycloheximide reversed 15d-PGJ (2)-mediated inhibition of Stat3 activation, actinomycin D had no effect on 15d-PGJ (2)-mediated inhibition of IL-6 signaling, indicating that inhibition of IL-6 signaling occur independent of de novo gene expression. |
4(0,0,0,4) | Details |
| 17353260 | Wang ZV, Schraw TD, Kim JY, Khan T, Rajala MW, Follenzi A, Scherer PE: Secretion of the adipocyte-specific secretory protein adiponectin critically depends on thiol-mediated protein retention. Mol Cell Biol. 2007 May;27(10):3716-31. Epub 2007 Mar 12. One mechanism for increasing circulating levels of specific adiponectin complexes by peroxisome proliferator-activated receptor gamma agonists may be selective upregulation of rate-limiting chaperones. |
1(0,0,0,1) | Details |
| 18281744 | Poczatkova H, Bogdanova K, Uherkova L, Cervenkova K, Riegrova D, Rypka M, Vesely J: effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells. Gen Physiol Biophys. 2007 Dec;26(4):268-74. To investigate the effect of on intracellular mRNA levels of peroxisome proliferator-activated receptors (PPARs) and PPAR-gamma coactivators (PGCs), we conducted a quantitative real-time RT-PCR study using HepG2 cells. Basal mRNA levels of PPAR-alpha and PGC-1alpha were significantly suppressed upon 20 h treatment with cycloheximide, while those of PPAR-gamma1, -gamma2, and PGC-1beta were elevated. |
1(0,0,0,1) | Details |
| 18501893 | Zeigerer A, Rodeheffer MS, McGraw TE, Friedman JM: Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism. Exp Cell Res. 2008 Jul 1;314(11-12):2249-56. Epub 2008 Apr 12. The addition of a PPARgamma agonist to the Isobutylmethylxanthine/Dexamethasone/Insulin differentiation cocktail increased leptin mRNA levels by 5-fold, maintained insulin sensitivity, and yielded mature phenotype in cultured adipocytes. |
1(0,0,0,1) | Details |
| 18643838 | Liu LF, Purushotham A, Wendel AA, Koba K, DeIuliis J, Lee K, Belury MA: Regulation of adipose triglyceride lipase by . Diabetes Obes Metab. 2009 Feb;11(2):131-42. Epub 2008 Jul 17. CONCLUSIONS: directly regulates transcription of ATGL, likely through a PPARgamma-mediated mechanism. Finally, we found that increased ATGL mRNA levels in 3T3-L1 adipocytes in the presence of cycloheximide, an inhibitor of protein synthesis, suggesting that regulation of ATGL occurs at the transcriptional level. |
1(0,0,0,1) | Details |
| 18345257 | Bogdanova K, Uherkova L, Poczatkova H, Rypka M, Vesely J: mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by deprivation and in human hepatoma hepG2 cells. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007 Dec;151(2):237-45. The aim of this study was to investigate the impact of energy abundant substrates on peroxisome proliferator-activated receptors (PPARs) and PPAR-gamma coactivators (PGCs) mRNA's steady-state levels. PPAR-gamma1 and -gamma2 mRNAs were significantly superinduced when the cells were treated with cycloheximide, whereas PPAR-alpha and PGC-1alpha and-1beta mRNAs were destabilized. |
1(0,0,0,1) | Details |