| Name | cyclooxygenase 2 |
|---|---|
| Synonyms | COX 2; COX2; PHS2; PGG/HS; Cyclooxygenase 2; Cyclooxygenase 2b; Cycloxygenase 2; PGH synthase 2… |
| Name | cycloheximide |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 19376214 | Ridley W, Matsuoka M: -induced cyclooxygenase-2 expression and production in A549 human pulmonary epithelial cells. Toxicol Lett. 2009 Aug 10;188(3):180-5. Epub 2009 Apr 17. NaF-induced accumulation of COX-2 transcript was abolished by actinomycin D, but not cycloheximide. |
2(0,0,0,2) | Details |
| 16123165 | Ishikawa T, Hwang K, Lazzarino D, Morris PL: Sertoli cell expression of steroidogenic acute regulatory protein-related lipid transfer 1 and 5 domain-containing proteins and sterol regulatory element binding protein-1 are interleukin-1beta regulated by activation of c-Jun N-terminal kinase and cyclooxygenase-2 and cytokine induction. Endocrinology. 2005 Dec;146(12):5100-11. Epub 2005 Aug 25. IL-1beta rapidly decreases levels of precursor and mature sterol regulatory element-binding protein-1, changes not altered by cycloheximide, suggesting coordinate regulation of StARD1 and -D5, but not StARD4, expression. |
2(0,0,0,2) | Details |
| 20006666 | Filipovich-Rimon T, Fleisher-Berkovich S: Protein synthesis dependent effects of kinins on astrocyte prostaglandin synthesis. Peptides. 2010 Apr;31(4):651-6. Epub 2009 Dec 16. These kinin effects on PGE (2) synthesis were completely abrogated by actinomycin-D and cycloheximide, suggesting de novo synthesis of proteins. Bradykinin also increased cyclooxygenase-2 protein levels about 2-fold, while the B (1) receptor agonist decreased cyclooxygenase-2 protein expression. |
2(0,0,0,2) | Details |
| 16934679 | Im JY, Kim D, Paik SG, Han PL: Cyclooxygenase-2-dependent neuronal death proceeds via generation. Free Radic Biol Med. 2006 Sep 15;41(6):960-72. Epub 2006 Jun 7. |
2(0,0,0,2) | Details |
| 19545424 | Liu T, Schneider RA, Shah V, Huang Y, Likhotvorik RI, Keshvara L, Hoyt DG: Protein Never in Mitosis Gene A Interacting-1 regulates calpain activity and the degradation of cyclooxygenase-2 in endothelial cells. J Inflamm. 2009 Jun 22;6:20. Cells were treated with LPS/IFN, calpain inhibitors (carbobenzoxy-valinyl-phenylalaninal (zVF), PD150606), cycloheximide and COX inhibitors to determine the effect of PIN1 depletion on COX-2 and calpain. |
2(0,0,0,2) | Details |
| 16198345 | Lim WC, Park M, Bahn JJ, Inoue H, Lee YJ: Hypertonic induction of cyclooxygenase-2 occurs independently of NF-kappaB and is inhibited by the glucocorticoid receptor in A549 cells. FEBS Lett. 2005 Oct 10;579(24):5430-6. This induction was a transcriptional event that occurred in the absence of the protein synthesis inhibitor cycloheximide and was the result of enhanced promoter activity, as examined with the use of full-length COX-2 promoter-driven reporter plasmids. |
2(0,0,0,2) | Details |
| 19318593 | Alvarez Y, Municio C, Alonso S, San Roman JA, Sanchez Crespo M, Fernandez N: Cyclooxygenase-2 induced by zymosan in human monocyte-derived dendritic cells shows high stability, and its expression is enhanced by J Pharmacol Exp Ther. 2009 Jun;329(3):987-94. Epub 2009 Mar 24. |
2(0,0,0,2) | Details |
| 16644485 | Nieminen R, Lahti A, Jalonen U, Kankaanranta H, Moilanen E: JNK inhibitor SP600125 reduces COX-2 expression by attenuating mRNA in activated murine J774 macrophages. Int Immunopharmacol. 2006 Jun;6(6):987-96. Epub 2006 Feb 9. Cycloheximide (that is known to activate JNK) enhanced COX-2 expression and its effect was inhibited by SP600125. Inducible prostaglandin synthase (cyclooxygenase-2, COX-2) is highly expressed in inflammation. |
1(0,0,0,1) | Details |
| 17477937 | Lim W, Jung J, Surh Y, Inoue H, Lee Y: Hypertonic and induces COX-2 via different signaling pathways in mouse cortical collecting duct M-1 cells. Life Sci. 2007 May 8;80(22):2085-92. Epub 2007 Apr 1. Previous studies have shown that, in renal medullary cells, hypertonic stress induces expression of cyclooxygenase-2 (COX-2) via NF-kappaB activation, but little is known about COX-2 expression in response to hypertonicity in the cortical collecting duct. The treatment also increased COX-2 mRNA accumulation in a cycloheximide-independent manner, suggesting that ongoing protein synthesis is not required for COX-2 induction. |
1(0,0,0,1) | Details |
| 17683115 | Fernandez N, Gonzalez A, Valera I, Alonso S, Crespo MS: Mannan and peptidoglycan induce COX-2 protein in human PMN via the mammalian target of rapamycin. Eur J Immunol. 2007 Sep;37(9):2572-82. The induction of cyclooxygenase-2 (COX-2) protein expression was assessed in human polymorphonuclear leukocytes (PMN) stimulated via receptors of the innate immune system. Treatment with the phosphatidylinositol 3-kinase inhibitor (PI3K) wortmaninn, the mammalian target of rapamycin (mTOR) inhibitor rapamycin, and the translation inhibitor cycloheximide blocked the induction of COX-2 protein in response to mannan and PGN, whereas the transcriptional inhibitor actinomycin D did not show a significant effect. |
1(0,0,0,1) | Details |
| 19800419 | Hwangbo C, Lee HS, Park J, Choe J, Lee JH: The anti-inflammatory effect of tussilagone, from Tussilago farfara, is mediated by the induction of heme oxygenase-1 in murine macrophages. Int Immunopharmacol. 2009 Dec;9(13-14):1578-84. Epub 2009 Oct 1. TSL-mediated HO-1 protein induction was not inhibited by treatment with actinomycin D, a transcriptional inhibitor, but by cycloheximide, a translational inhibitor. Consistent with the notion that HO-1 has anti-inflammatory properties, TSL inhibited the production of (NO), tumor necrosis factor (TNF)-alpha, and as well as inducible synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and murine peritoneal macrophages. |
1(0,0,0,1) | Details |
| 16696851 | Tang HB, Inoue A, Iwasa M, Hide I, Nakata Y: Substance P release evoked by or from rat cultured dorsal root ganglion neurons is conversely modulated with bradykinin. J Neurochem. 2006 Jun;97(5):1412-8. Bradykinin-evoked SP release from cultured adult rat DRG neurons was attenuated by either the mitogen-activated protein kinase kinase (MEK) inhibitor (U0126) or cycloheximide. As the long-term exposure of DRG neurons to bradykinin (3 h) resulted in extracellular signal-regulated kinase (ERK) phosphorylation at an early stage and thereafter induced cyclooxygenase-2 (COX-2) protein expression, which both contribute to the SP release triggered by bradykinin B2 receptor. |
1(0,0,0,1) | Details |
| 18312133 | Menzies-Gow NJ, Bailey SR, Berhane Y, Brooks AC, Elliott J: Evaluation of the induction of vasoactive mediators from equine digital vein endothelial cells by endotoxin. Am J Vet Res. 2008 Mar;69(3):349-55. The EDVECs were incubated for 18 hours with LPS (10 pg/mL to 1 microg/mL) with or without cycloheximide, or L-nitroarginine methyl ester. Changes in inducible synthase and cyclooxygenase-2 expression were determined. |
1(0,0,0,1) | Details |
| 16606835 | Uzonyi B, Lotzer K, Jahn S, Kramer C, Hildner M, Bretschneider E, Radke D, Beer M, Vollandt R, Evans JF, Funk CD, Habenicht AJ: Cysteinyl leukotriene 2 receptor and protease-activated receptor 1 activate strongly correlated early genes in human endothelial cells. Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6326-31. Epub 2006 Apr 10. Transcripts peaked at approximately 60 min, were unaffected by a cysLT1-R antagonist, and were superinduced by cycloheximide. Strongly induced genes were studied in detail: Early growth response (EGR) and nuclear receptor subfamily 4 group A transcription factors; E-selectin; CXC ligand 2; IL-8; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1 (ADAMTS1); Down syndrome critical region gene 1 (DSCR1); tissue factor (TF); and cyclooxygenase 2. |
1(0,0,0,1) | Details |
| 19337795 | Wang Y, Chen B, Shen D, Xue S: Osteopontin protects against cardiac ischemia-reperfusion injury through late preconditioning. Heart Vessels. 2009 Mar;24(2):116-23. Epub 2009 Apr 1. The protein synthesis inhibitor cycloheximide (CH) was used in this model to test if new protein synthesis was necessary for its cardioprotective effects. The effector proteins in late preconditioning, including inducible synthase and cyclooxygenase-2, were evaluated by immunoblotting. |
1(0,0,0,1) | Details |
| 16146692 | Yasuda T, Kanno M, Kawamoto M, Yuge O, Ninomiya Y: Suppression of inducible synthase and cyclooxygenase-2 gene expression by 22 (R)-hydroxycholesterol requires de novo protein synthesis in activated macrophages. J Steroid Biochem Mol Biol. 2005 Dec;97(4):376-83. Epub 2005 Sep 16. Furthermore iNOS and COX-2 mRNA suppression by 22R-HC was diminished by cellular treatment with cycloheximide. |
1(0,0,0,1) | Details |
| 16464966 | Balzary RW, Cocks TM: Lipopolysaccharide induces epithelium- and (2)-dependent relaxation of mouse isolated trachea through activation of cyclooxygenase (COX)-1 and COX-2. J Pharmacol Exp Ther. 2006 May;317(2):806-12. Epub 2006 Feb 7. Because NF-kappaB induces cyclooxygenase-2 (COX-2) to increase synthesis of prostaglandins (PGs), including the potent airway anti-inflammatory and smooth muscle relaxant PGE (2), we investigated whether LPS causes short-term PGE (2)-dependent relaxation of mouse isolated trachea. The LPS antagonist polymixin B; the nonselective COX inhibitor indomethacin; the selective COX-1 and COX-2 inhibitors 5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole (SC560) and 4-[5-(4-chlorophenyl)-1-(trifluoromethyl)-1H-pyrazol-1-yl]-benzenesulfonam ide (SC236), respectively; the transcription inhibitor actinomycin D; the translation inhibitor cycloheximide; the p38 mitogen-activated protein kinase (p38 MAPK) inhibitor 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imadazole (SB203580); and a combination of the mixed DP/EP1/EP2 receptor antagonist 6-isopropoxy-9-xanthone-2-carboxylic acid (AH6809) and the EP4 receptor antagonist 4'-[3-butyl-5-oxo-1-(2-trifluoromethyl-phenyl)-1-5-dihydro-[1,2,4] triazol- 4-ylmethyl]-biphenyl-2- (3-methyl-thiophene-2-carbonyl)-amide (L-161982) all abolished relaxation to LPS, giving instead slowly developing, small contractions over 60 min. |
1(0,0,0,1) | Details |
| 20036891 | Castillo-Hernandez MC, Guevara-Balcazar G, Lopez-Sanchez P, Asbun-Bojalil J, Lopez RM, Castillo EF, Castillo-Henkel C: The influence of constitutive COX-2 in smooth muscle tissue on the contractile effect of in the rat abdominal aorta. Front Biosci (Elite Ed). 2010 Jan 1;2:441-8. Both cyclooxygenase isoforms (COX-1 and COX-2) were expressed in the two aortic segments, but their expression was not altered by the protein synthesis inhibitor cycloheximide, or the phospholipase A2 inhibitors arachidonyl trifluoromethyl ketone and methyl arachidonyl fluorophosponate. |
0(0,0,0,0) | Details |