Protein Information

Name TRPV6
Synonyms ABP/ZF; CAT1; CATL; CaT L; CaT like; Calcium transport protein 1; Calcium transport protein CaT1; Calcium transporter 1…

Compound Information

Name cycloheximide
CAS

Reference List

PubMed Abstract RScore(About this table)
16362534 Taparia S, Fleet JC, Peng JB, Wang XD, Wood RJ: 1,25-Dihydroxyvitamin D and 25-hydroxyvitamin D--mediated regulation of TRPV6 (a putative epithelial calcium channel) mRNA expression in Caco-2 cells. Eur J Nutr. 2006 Jun;45(4):196-204. Epub 2005 Dec 21.

METHODS: Caco-2 cells were treated in a 2 x 2 format with 1,25VD and the transcriptional inhibitor actinomycin D (AD, 4 microg/ml), and also with translational inhibitor cycloheximide (CHX, 10 microg/ml) after 14 days in culture and TRPV6 mRNA levels were determined using reverse transcription-real time PCR.TRPV6 mRNA half life studies were performed by inhibiting transcription followed by sampling at various time points for TRPV6 mRNA.
39(0,1,1,9) Details
16002434 Wang TT, Tavera-Mendoza LE, Laperriere D, Libby E, MacLeod NB, Nagai Y, Bourdeau V, Konstorum A, Lallemant B, Zhang R, Mader S, White JH: Large-scale in silico and microarray-based identification of direct 1,25-dihydroxyvitamin D3 target genes. Mol Endocrinol. 2005 Nov;19(11):2685-95. Epub 2005 Jul 7.

Hu133A microarray analyses, performed with RNA from human SCC25 cells treated with 1,25 (OH) 2D3 and protein synthesis inhibitor cycloheximide, identified more than 900 regulated genes.
These experiments also revealed 16 VDREs in 11 of 12 genes induced more than 10-fold in our previous microarray study, five elements in the human gene encoding the epithelial calcium channel TRPV6, as well as novel 1,25 (OH2) D3 target genes implicated in regulation of cell cycle progression.
1(0,0,0,1) Details