| 17540704 |
Shen C, Lin MJ, Yaradanakul A, Lariccia V, Hill JA, Hilgemann DW: Dual control of cardiac Na+ Ca2+ exchange by PIP (2): analysis of the surface membrane fraction by extracellular cysteine PEGylation. J Physiol. 2007 Aug 1;582(Pt 3):1011-26. Epub 2007 May 31.
An extracellular NCX1 disulphide bond is rapidly reduced by tris (2-carboxyethyl) phosphine hydrochloride (TCEP), cysteines are 'PEGylated' by alkylation with an impermeable conjugate of maleimide and a 5000 MW polyethylene glycol (MPEG), and F (surf) is quantified from Western blots as the fraction of NCX1 that migrates at a higher molecular weight. |
86(1,1,1,6) |
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