Protein Information

Name protein a
Synonyms A1 activator; PSAP; Protein C; CSAct; Cerebroside sulfatase activator; Cerebroside sulfate activator; Co beta glucosidase; Component C…

Compound Information

Name simazine
CAS

Reference List

PubMed Abstract RScore(About this table)
10464482 Yazynina EV, Zherdev AV, Dzantiev BB, Izumrudov VA, Gee SJ, Hammock BD: Immunoassay techniques for detection of the herbicide simazine based on use of oppositely charged water-soluble polyelectrolytes. Anal Chem. 1999 Aug 15;71(16):3538-43.

A tracer antigen (simazine) was allowed to interact in solution with components of the reaction mixture containing simazine-peroxidase conjugate, specific antibodies, and staphylococcal protein A conjugated with the polyanion, and then the mixture was added to the immobilized polycation.
81(1,1,1,1) Details
10225515 Choi MJ, Jo Y, Choi J, Kang CY, Han CT: Production and characterization of monoclonal antibodies specific to atrazine group compounds: effects of coating ligand structure on the variation of sensitivity and specificity. J Immunoassay. 1999 Feb-May;20(1-2):57-77.

The protein A affinity purification yielded 0.35-0.65 mg per ml of ascitic fluid from each cell line.
The characterization studies in terms of sensitivity and specificity indicate that MAb 2F9 and MAb 4B9 showed the best responses with atrazine and its group of ametryne and cyanazine, using microtiter plate coated with simazine derivative of 6-amino hexanoic acid; no cross-reactivity was shown with simazine and cyanuric chloride.
1(0,0,0,1) Details
18967431 Turiel E, Fernandez P, Perez-Conde C, Gutierrez AM, Camara C: Flow-through fluorescence immunosensor for atrazine determination. Talanta. 1998 Dec;47(5):1255-61.

A new flow-through fluoroimmunosensor for atrazine determination based on the use of protein A immobilized on controlled pore glass as immunoreactor is reported.
Possible interferences of other triazines like simazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) were evaluated.
1(0,0,0,1) Details