Protein Information

Name D lactate dehydrogenase
Synonyms D lactate dehydrogenase; DLD; LDHD; Lactate dehydrogenase D; Lactate dehydrogenase Ds

Compound Information

Name oxamate
CAS hexyl 2-(diethylamino)-2-oxoacetate

Reference List

PubMed Abstract RScore(About this table)
4579004 Reeves JP, Hong JS, Kaback HR: Reconstitution of D-lactate-dependent transport in membrane vesicles from a D-lactate dehydrogenase mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1917-21.

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6402502 Mueller PR, Collins ML: Identification of two distinct lactate dehydrogenases in Rhodospirillum rubrum. J Bacteriol. 1983 Mar;153(3):1562-6.

Crossed immunoelectrophoretic analysis revealed two antigenically distinct enzymes that were further distinguished by specificity for d- and l-stereoisomers of lactate and by the sensitivity of the d-lactate dehydrogenase to inhibition by oxamate and oxalate.
81(1,1,1,1) Details
9399585 Taguchi H, Ohta T, Matsuzawa H: Involvement of Glu-264 and Arg-235 in the essential interaction between the catalytic imidazole and substrate for the D-lactate dehydrogenase catalysis. J Biochem. 1997 Oct;122(4):802-9.

The replacement of Arg-235 with Gln destabilized the inhibitory binding of oxamate much more than that of formate, acetate, or propionate, and the Arg to Lys substitution specifically diminished only oxamate binding.
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168830 Sanchez JJ, Palleroni NJ, Doudoroff M: Lactate dehydrogenases in cyanobacteria. Arch Microbiol. 1975 Jun 20;104(1):57-65.


The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied.
1(0,0,0,1) Details
11396932 Jasso-Chavez R, Torres-Marquez ME, Moreno-Sanchez R: The Membrane-bound L- and D-lactate dehydrogenase activities in mitochondria from Euglena gracilis. Arch Biochem Biophys. 2001 Jun 15;390(2):295-303.

Oxamate was a competitive inhibitor against both L- and D-lactate.
1(0,0,0,1) Details
7961609 Taguchi H, Ohta T: Essential role of arginine 235 in the substrate-binding of Lactobacillus plantarum D-lactate dehydrogenase. J Biochem. 1994 May;115(5):930-6.

Both the mutant enzymes showed a marked resistance to 2,3-butanedione, by which the wild-type enzyme is rapidly inactivated unless NADH and oxamate are present.
1(0,0,0,1) Details