| Name | beta glucuronidase |
|---|---|
| Synonyms | Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors |
| Name | thidiazuron |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 15235813 | Zhao Y, Liu Q, Davis RE: Transgene expression in strawberries driven by a heterologous phloem-specific promoter. Plant Cell Rep. 2004 Oct;23(4):224-30. Epub 2004 Jul 2. For targeted phloem-specific expression, we constructed a binary vector with an expression cassette bearing the beta-glucuronidase (GUS) reporter gene (uidA) under control of the Arabidopsis -H+ symporter gene (AtSUC2) promoter. Transgenic strawberry lines were generated with high efficiencies by a modified transformation protocol, which combines the adoption of a 3-day pre-selection period following transformation, and the addition of 10-microM thidiazuron to the regeneration medium. |
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| 11760772 | Egnin M, Mora A, Prakash CS: Factors enhancing Agrobacterium tumefaciens-mediated gene transfer in peanut (Arachis hypogaea L.). In Vitro Cell Dev Biol Plant. 1998 Oct-Dec;34(4):310-8. An intron-containing beta-glucuronidase uidA (gusA) gene under the transcriptional control of CaMV 35S promoter served as a reporter. The use of cocultivation media containing high auxin [1.0 or 2.5 mg/l (4.53 micromolar or 11.31 micromolar) 2,4-D] and low cytokinin [0.25 or 0.5 mg/l (1.0 micromolar or 2.0 micromolar) BA] promoted higher transformation than either hormone-free or thidiazuron-containing medium. |
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| 17492451 | Cseke LJ, Cseke SB, Podila GK: High efficiency poplar transformation. Plant Cell Rep. 2007 Sep;26(9):1529-38. Epub 2007 May 11. Our method uses Agrobacterium inoculated aspen seedling hypocotyls followed by direct thidiazuron (TDZ)-mediated shoot regeneration on selective media. Transformation was verified through beta-glucuronidase (GUS) reporter gene expression in all tree tissues, PCR amplification of appropriate vector products from isolated genomic DNA, and northern hybridization of incorporated and expressed transgenes. |
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| 19343350 | Du N, Pijut PM: Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration. Plant Cell Rep. 2009 Jun;28(6):915-23. Epub 2009 Apr 3. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and an enhanced green fluorescent protein gene. Adventitious shoots regenerated on Murashige and Skoog medium supplemented with 13.3 microM 6-benzylaminopurine, 4.5 microM thidiazuron, 50 mg l (-1) and 10% coconut water. |
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| 12548800 | Wang LJ, Ni DA, Wang GY, Xia ZA, Xu ZK: [Preliminary studies on tissue culture and agrobacterium-medicated transformation of Brassica campestris ssp. chinensis]. Shi Yan Sheng Wu Xue Bao. 1999 Mar;32(1):93-9. After 4-5 weeks, the differentiated buds were transferred onto selection medium with CP 200 mg/L and Km 10 mg/L for 1 month, then the green shoots were transferred onto the rooting medium containing Cef 100 mg/L and Km 20 mg/L. 4-5 weeks later, plantlets with Km resistance were obtained and some of them showed higher enzymatic activities of beta-glucuronidase than control ones. |
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| 12789429 | Franklin G, Lakshmi Sita G: Agrobacterium tumefaciens-mediated transformation of eggplant (Solanum melongena L.) using root explants. Plant Cell Rep. 2003 Feb;21(6):549-54. Epub 2003 Jan 8. Root explants were transformed by co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring a binary vector pBAL2 carrying the reporter gene beta-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calli were induced in media containing 0.1 mg l (-1) thidiazuron (TDZ), 3.0 mg l (-1) N (6)-benzylaminopurine, 100 mg l (-1) kanamycin and 500 mg l (-1) cefotaxime. |
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| 18626648 | Sailaja M, Tarakeswari M, Sujatha M: Stable genetic transformation of castor (Ricinus communis L.) via particle gun-mediated gene transfer using embryo axes from mature seeds. 25: id: 18426466 Error occurred: Error 11 (Resource temporarily unavailable)Plant Cell Rep. 2008 Sep;27(9):1509-19. Epub 2008 Jul 15. Decotyledonated embryos from mature seeds were germinated and the embryonic axis was induced to proliferate on Murashige and Skoog (MS) medium supplemented with 0.5 mg l (-1) thidiazuron (TDZ) and subjected to bombardment after 5-7 days of pre-incubation. The physical parameters for transient transformation were optimized using the UidA gene encoding beta-glucuronidase (GUS) as the reporter gene and with hygromycin-phosphotransferase (hptII) gene as selectable marker. |
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| 15300402 | Song GQ, Sink KC: Agrobacterium tumefaciens-mediated transformation of blueberry (Vaccinium corymbosum L.). Plant Cell Rep. 2004 Dec;23(7):475-84. Epub 2004 Aug 5. Transient expression studies using blueberry leaf explants and monitored by beta-glucuronidase (GUS) assays indicated Agrobacterium tumefaciens strain EHA105 was more effective than LBA4404 or GV3101; and the use of acetosyringone (AS) at 100 microM for inoculation and 6 days co-cultivation was optimum compared to 2, 4, 8, 10 or 12 days. Explants were then placed on modified WPM supplemented with 1.0 mg l (-1) thidiazuron, 0.5 mg l (-1) alpha-naphthaleneacetic, 10 mg l (-1) kanamycin (Km), and 250 mg l (-1) cefotaxime. |
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