| 17459612 |
Singh D, Kwon HJ, Rajkarnikar A, Suh JW: Glucoamylase gene, vldI, is linked to validamycin biosynthesis in Streptomyces hygroscopicus var. limoneus, and vldADEFG confers validamycin production in Streptomyces lividans, revealing the role of VldE in glucose attachment. Gene. 2007 Jun 15;395(1-2):151-9. Epub 2007 Mar 12.
Ion-exchange chromatographic purification and a subsequent HPLC analysis confirmed that S. lividans/vldADEFG yielded a 75 microg/l of validamycin-A, showing that the validamycin pathway involves a single NDP-sugar glycosyltransferase reaction. |
6(0,0,1,1) |
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| 16725283 |
Singh D, Seo MJ, Kwon HJ, Rajkarnikar A, Kim KR, Kim SO, Suh JW: Genetic localization and heterologous expression of validamycin biosynthetic gene cluster isolated from Streptomyces hygroscopicus var. limoneus KCCM 11405 (IFO 12704). Gene. 2006 Jul 5;376(1):13-23. Epub 2006 Apr 5.
Located around vldA, vldB to K were predicted to encode adenyltransferase, kinase, ketoreductase (or epimerase/dehydratase), glycosyltransferase, aminotransferase, dehydrogenase, phosphatase/phosphomutase, glycosyl hydrolase, transport protein, and glycosyltransferase, respectively. |
1(0,0,0,1) |
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