Protein Information

Name beta glucosidase
Synonyms CBG; CBG; GLUC; CBGL 1; CBGL1; Cytosolic beta glucosidase; Cytosolic beta glucosidase like protein 1; GBA 3…

Compound Information

Name zeatin
CAS

Reference List

PubMed Abstract RScore(About this table)
16667800 Nandi SK, Palni LM, Parker CW: Dynamics of Endogenous Cytokinins during the Growth Cycle of a Hormone-Autotrophic Genetic Tumor Line of Tobacco. Plant Physiol. 1990 Nov;94(3):1084-1089.

Other cytokinins quantified were dihydrozeatin, zeatin riboside, and dihydrozeatin riboside; the values may include contributions from aglucones derived from the hydrolysis of corresponding O-glucosides, since the entire basic fraction was treated with beta-glucosidase before analysis.
6(0,0,1,1) Details
10948220 Dietz KJ, Sauter A, Wichert K, Messdaghi D, Hartung W: Extracellular beta-glucosidase activity in barley involved in the hydrolysis of ABA glucose conjugate in leaves. J Exp Bot. 2000 May;51(346):937-44.

Inhibition was highest for the ABA glucopyranoside and the zeatin riboside, but insignificant for ABA methyl ester and zeatin-9-beta-D-glucoside.
5(0,0,0,5) Details
16488914 Kiran NS, Polanska L, Fohlerova R, Mazura P, Valkova M, Smeral M, Zouhar J, Malbeck J, Dobrev PI, Machackova I, Brzobohaty B: Ectopic over-expression of the maize beta-glucosidase Zm-p60.1 perturbs cytokinin homeostasis in transgenic tobacco. J Exp Bot. 2006;57(4):985-96. Epub 2006 Feb 17.

O-glucosides of zeatin-type cytokinins are postulated to be storage and/or transport forms, and are readily deglucosylated.
2(0,0,0,2) Details
16770627 Gu R, Zhao L, Zhang Y, Chen X, Bao J, Zhao J, Wang Z, Fu J, Liu T, Wang J, Wang G: Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Plant Cell Rep. 2006 Nov;25(11):1157-65. Epub 2006 Jun 13.

The results also showed that this promoter could be inhibited by 6-BA, trans-zeatin, and NAA, but was not affected by GA (3), ABA, SA, cold, salt, drought, and submergence treatments.
2(0,0,0,2) Details
17175552 Polanska L, Vicankova A, Novakova M, Malbeck J, Dobrev PI, Brzobohaty B, Vankova R, Machackova I: Altered cytokinin metabolism affects cytokinin, auxin, and abscisic acid contents in leaves and chloroplasts, and chloroplast ultrastructure in transgenic tobacco. J Exp Bot. 2007;58(3):637-49. Epub 2006 Dec 14.

CK-O-glucosides accumulated to very high levels in leaves, but not in chloroplasts, of plants overexpressing a ZOG1 gene, encoding trans-zeatin-O-glucosyltransferase from Phaseolus lunatus.
In a transformant overexpressing a maize beta-glucosidase gene, Zm-p60.1, naturally targeted to plastids, a decrease of CK-O-glucosides in chloroplasts was found.
1(0,0,0,1) Details
8235622 Brzobohaty B, Moore I, Kristoffersen P, Bako L, Campos N, Schell J, Palme K: Release of active cytokinin by a beta-glucosidase localized to the maize root meristem. Science. 1993 Nov 12;262(5136):1051-4.

1(0,0,0,1) Details
16157652 Novakova M, Motyka V, Dobrev PI, Malbeck J, Gaudinova A, Vankova R: Diurnal variation of cytokinin, auxin and abscisic acid levels in tobacco leaves. J Exp Bot. 2005 Nov;56(421):2877-83. Epub 2005 Sep 12.

These cytokinin oxidase/dehydrogenase peaks were accompanied by increased activity of beta-glucosidase (EC 3.2.1.21), which might be involved in the hydrolysis of cytokinin O-glucosides and/or in fine-tuning of active cytokinin levels at their midday peak.
The content of total cytokinins (mainly N-glucosides, followed by cis-zeatin derivatives and nucleotides) exhibited the main peak after 9 h of light and the minor peak after the transition to darkness.
1(0,0,0,1) Details
14972999 Doumas P, Bonnet-Masimbert M, Zaerr JB: Evidence of cytokinin bases, ribosides and glucosides in roots of Douglas-fir, Pseudotsuga menziesii. Tree Physiol. 1989 Mar;5(1):63-72.

Cytokinin bases and ribosides were estimated by radioimmunoassay after purification by immunoaffinity (zeatin riboside and isopentenyladenosine antibodies coupled to cellulose gel) and high performance liquid chromatography.
Cytokinin glucosides were similarly analyzed in root extracts treated with beta-glucosidase and octadecyl-silica column chromatography in conjunction with immunoaffinity purification.
1(0,0,0,1) Details
7659745 Falk A, Rask L: Expression of a zeatin-O-glucoside-degrading beta-glucosidase in Brassica napus. Plant Physiol. 1995 Aug;108(4):1369-77.

Zeatin-O-glucoside was identified as a natural substrate for B. napus beta-glucosidase.
92(1,1,2,7) Details
19933382 Saleem M, Lamkemeyer T, Schutzenmeister A, Madlung J, Sakai H, Piepho HP, Nordheim A, Hochholdinger F: Specification of cortical parenchyma and stele of maize primary roots by asymmetric levels of auxin, cytokinin, and cytokinin-regulated proteins. Plant Physiol. 2010 Jan;152(1):4-18. Epub 2009 Nov 20.

A root-specific beta-glucosidase that functions in the hydrolysis of cis-zeatin O-glucoside was preferentially accumulated in the cortical parenchyma.
31(0,1,1,1) Details
12646503 Lexa M, Genkov T, Malbeck J, Machackova I, Brzobohaty B: Dynamics of endogenous cytokinin pools in tobacco seedlings: a modelling approach. Ann Bot. 2003 Apr;91(5):585-97.

Individual types of cytokinins in plants are interconverted to their respective forms by several enzymatic activities (5'-AMP-isopentenyltransferase, adenosine nucleosidase, 5'-nucleotidase, adenosine phosphorylase, adenosine kinase, trans-hydroxylase, zeatin reductase, beta-glucosidase, O-glucosyl transferase, N-glucosyl transferase, cytokinin oxidase).
6(0,0,1,1) Details