Protein Information

Name transferase
Synonyms 4' phosphopantetheinyl transferase; 4' phosphopantetheinyl transferase; AASD PPT; AASDHPPT; AASDPPT; Alpha aminoadipic semialdehyde dehydrogenase phosphopantetheinyl transferase; Aminoadipate semialdehyde dehydrogenase phosphopantetheinyl transferase; CGI 80…

Compound Information

Name zeatin
CAS

Reference List

PubMed Abstract RScore(About this table)
16665104 Macdonald EM, Powell GK, Regier DA, Glass NL, Roberto F, Kosuge T, Morris RO: Secretion of Zeatin, Ribosylzeatin, and Ribosyl-1'' -Methylzeatin by Pseudomonas savastanoi: Plasmid-Coded Cytokinin Biosynthesis. Plant Physiol. 1986 Nov;82(3):742-747.

A fragment of the 105 kilobase pair plasmid from EW1006 was cloned into Escherichia coli where its expression resulted in dimethylallyl transferase activity and the secretion of zeatin.
81(1,1,1,1) Details
12359893 Medford JI, Horgan R, El-Sawi Z, Klee HJ: Alterations of Endogenous Cytokinins in Transgenic Plants Using a Chimeric Isopentenyl Transferase Gene. Plant Cell. 1989 Apr;1(4):403-413.

Heat induction of transgenic plants caused the isopentenyl transferase mRNA to accumulate and increased the level of zeatin 52-fold, zeatin riboside 23-fold, and zeatin riboside 5 [prime]-monophosphate twofold.
9(0,0,1,4) Details
12646503 Lexa M, Genkov T, Malbeck J, Machackova I, Brzobohaty B: Dynamics of endogenous cytokinin pools in tobacco seedlings: a modelling approach. Ann Bot. 2003 Apr;91(5):585-97.

Individual types of cytokinins in plants are interconverted to their respective forms by several enzymatic activities (5'-AMP-isopentenyltransferase, adenosine nucleosidase, 5'-nucleotidase, adenosine phosphorylase, adenosine kinase, trans-hydroxylase, zeatin reductase, beta-glucosidase, O-glucosyl transferase, N-glucosyl transferase, cytokinin oxidase).
6(0,0,1,1) Details
2980282 Powell GK, Hommes NG, Kuo J, Castle LA, Morris RO: Inducible expression of cytokinin biosynthesis in Agrobacterium tumefaciens by plant phenolics. Mol Plant Microbe Interact. 1988 Jul-Aug;1(6):235-42.

Nopaline strains of Agrobacterium tumefaciens contain a gene, tzs, that encodes a cytokinin biosynthetic prenyl transferase.
As a result, bacteria containing tzs secrete low levels of zeatin into the medium.
2(0,0,0,2) Details
1888890 Smigocki AC: Cytokinin content and tissue distribution in plants transformed by a reconstructed isopentenyl transferase gene. Plant Mol Biol. 1991 Jan;16(1):105-15.

Zeatin and zeatinriboside concentrations 2 hours after heat shock ranged from over 900 to 2000 pmol/g, representing a greater than 140- to 200-fold increase over uninduced levels.
2(0,0,0,2) Details
12226381 Redig P, Schmulling T, Van Onckelen H: Analysis of Cytokinin Metabolism in ipt Transgenic Tobacco by Liquid Chromatography-Tandem Mass Spectrometry. Plant Physiol. 1996 Sep;112(1):141-148.

The endogenous levels of the major, naturally occurring cytokinins in Pisum sativum ribulose-1,5-bisphosphate carboxylase small subunit promoter-isopentenyl transferase gene (Pssu-ipt)-transformed tobacco (Nicotiana tabacum L.) callus were quantified using electrospray-liquid chromatography-tandem mass spectrometry during a 6-week subcultivation period.
Activation of the ipt in both expression systems resulted in the production of mainly zeatin-type cytokinins.
1(0,0,0,1) Details
7888614 Thomas JC, Smigocki AC, Bohnert HJ: Light-induced expression of ipt from Agrobacterium tumefaciens results in cytokinin accumulation and osmotic stress symptoms in transgenic tobacco. Plant Mol Biol. 1995 Jan;27(2):225-35.

To test whether increased endogenous cytokinin accumulation led to NaCl stress symptoms, the gene ipt from Agrobacterium tumefaciens, encoding isopentenyl transferase, was transformed into Nicotiana tabacum cv.
In high light (300 mumol PPFD m-2 s-1), ipt mRNA was detected and zeatin/zeatin glucoside levels were 10-fold higher than in control plants or when transformants were grown in low light (30 mumol PPFD m-2 s-1).
1(0,0,0,1) Details
18184738 Galichet A, Hoyerova K, Kaminek M, Gruissem W: Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis. Plant Physiol. 2008 Mar;146(3):1155-64. Epub 2008 Jan 9.

Moreover, AtIPT3 is a substrate of the protein farnesyl transferase, and AtIPT3 farnesylation directed the localization of the protein in the nucleus/cytoplasm, whereas the nonfarnesylated protein was located in the plastids.
AtIPT3 gain-of-function mutant analysis indicated that the different subcellular localization of the farnesylated protein and the nonfarnesylated protein was closely correlated with either isopentenyl-type or zeatin-type cytokinin biosynthesis.
1(0,0,0,1) Details
8219068 Smigocki A, Neal JW Jr, McCanna I, Douglass L: Cytokinin-mediated insect resistance in Nicotiana plants transformed with the ipt gene. Plant Mol Biol. 1993 Oct;23(2):325-35.

Zeatin and zeatinriboside levels in leaves remaining on PI-II-ipt plants after hornworm feeding were elevated by about 70-fold and the chlorophyll a/b content was double that of controls.
The bacterial isopentenyl transferase (ipt) gene involved in cytokinin biosynthesis was fused with a promoter from the proteinase inhibitor II (PI-IIK) gene and introduced into Nicotiana plumbaginifolia.
1(0,0,0,1) Details