Protein Information

Name lysozyme
Synonyms LYZ; LZM; Lysozyme; Lysozyme C; Lysozyme C precursor; Lysozymes; Lysozyme Cs; Lysozyme C precursors

Compound Information

Name potassium thiocyanate
CAS potassium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
6838037 Brogden KA, Rimler RB: Lysates of turkey-grown Pasteurella multocida: effects of solubilizing agents on the immunologic properties of membrane vesicles. Am J Vet Res. 1983 Mar;44(3):428-32.

Potassium thiocyanate, NaOH-glycine, lithium diiodosalicylate, guanidine hydrochloride, n-butanol, dimethyl sulfoxide, Triton X-100, and sodium lauryl sarcosinate were each tested as solubilizing agents.
The external morphology of P multocida, after treatment with lysozyme and EDTA, was similar whether grown in broth or in turkeys.
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873944 Wedler FC: Analysis of biomaterials deposited on soft contact lenses. J Biomed Mater Res. 1977 Jul;11(4):525-35.

Urea, guanidine hydrochloride, potassium thiocyanate, potassium perchlorate, hydroxylamine, and EDTA were much less effective than SDS and DTT.
Fluorescent antibody tests with appropriate controls gave positive tests for albumin, lysozyme, gamma-G-globulin, and alpha1-lipoprotein in the deposits, all proteins present in tear fluid.
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11291029 Tobler SA, Sherman NE, Fernandez EJ: Tracking lysozyme unfolding during salt-induced precipitation with hydrogen exchange and mass spectrometry. Biotechnol Bioeng. 2000-2001;71(3):194-207.

Deuterated lysozyme was dissolved in protonated buffer at pH 2.16 and precipitated with ammonium sulfate, sodium chloride, and potassium thiocyanate.
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11321161 Xu Y, Lu J, Li DQ: The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4) 2SO4 aqueous two-phase system. Prep Biochem Biotechnol. 2001 Feb;31(1):23-31.

The effect of potassium thiocyanate on the partitioning of lysozyme and BSA in polyethylene glycol 2000/ammonium sulfate aqueous two-phase system has been investigated.
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1663504 Wirth HJ, Unger KK, Hearn MT: High-performance liquid chromatography of amino acids, peptides and proteins. J Chromatogr. 1991 Jul 26;550(1-2):383-95.

The elution was performed in each case with a 2.5 M potassium thiocyanate solution.
The capacities and the association constants associated with the binding of lysozyme to these sorbents were determined by frontal analysis experiments [J.
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12885620 Bostrom M, Williams DR, Ninham BW: Specific ion effects: why the properties of lysozyme in salt solutions follow a Hofmeister series. Biophys J. 2003 Aug;85(2):686-94.

Why for instance does the use of two different monovalent salts, potassium thiocyanate and potassium chloride, produce such different results? One important and previously neglected source of ion specificity is the ionic dispersion potential that acts between each ion and the protein.
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15299795 Howell PL: Structure of hexagonal turkey egg-white lysozyme at 1.65A resolution. Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):654-62.

The crystals were grown from a 150 mM potassium thiocyanate solution at pH 4.5 and belong to space group P6 (1) 22 with unit-cell dimensions a = b = 70.96, c = 83.01 A alpha = beta = 90, gamma = 120 degrees.
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2331471 Arakawa T, Bhat R, Timasheff SN: Preferential interactions determine protein solubility in three-component solutions: the MgCl2 system. Biochemistry. 1990 Feb 20;29(7):1914-23.


Preferential interaction and solubility measurements with three proteins, beta-lactoglobulin, bovine serum albumin, and lysozyme, resulted in similar patterns of interaction.
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