Protein Information

Name glutamate receptor
Synonyms GRINL1A; GUP1; GUP2; GRINL1A combined protein; GRINL1A combined protein Gcom10; GRINL1A combined protein Gcom10 precursor; GRINL1A combined protein Gcom11; GRINL1A combined protein Gcom11 precursor…

Compound Information

Name potassium thiocyanate
CAS potassium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
2542455 Henley JM, Barnard EA: Solubilisation and characterisation of a putative quisqualate-type glutamate receptor from chick brain. J Neurochem. 1989 Jul;53(1):140-8.

The brains of 1-day-old chicks were shown to be a rich source of binding sites with the pharmacological characteristics expected of a quisqualate-type glutamate receptor. alpha-[3H] Amino-3-hydroxy-5-methylisoxazolepropionate ([3H] AMPA) bound with KD and Bmax values, measured at 0 degree C in the presence of the chaotrope potassium thiocyanate, of 55 nM and 2.6 pmol/mg protein.
32(0,1,1,2) Details
1967632 Nielsen EO, Drejer J, Cha JH, Young AB, Honore T: Autoradiographic characterization and localization of quisqualate binding sites in rat brain using the antagonist [3H] 6-cyano-7-nitroquinoxaline-2,3-dione: comparison with (R,S)-[3H] alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid binding sites. J Neurochem. 1990 Feb;54(2):686-95.

Using quantitative autoradiography, we have investigated the binding sites for the potent competitive non-N-methyl-D-aspartate (non-NMDA) glutamate receptor antagonist [3H] 6-cyano-7-nitro-quinoxaline-2,3-dione ([3H]-CNQX) in rat brain sections. [3H] CNQX binding was regionally distributed, with the highest levels of binding present in hippocampus in the stratum radiatum of CA1, stratum lucidum of CA3, and molecular layer of dentate gyrus.
Binding of [3H] AMPA to quisqualate receptors in the presence of potassium thiocyanate produced curvilinear Scatchard plots.
2(0,0,0,2) Details
7680260 Kataoka K, Mitani A, Andou Y, Enomoto R, Ogita K, Yoneda Y: Binding of [3H] MK-801, NMDA-displaceable [3H] glutamate, [3H] glycine, [3H] spermidine, [3H] kainate and [3H] AMPA to regionally discrete brain membranes of the gerbil: a biochemical study. Neurochem Int. 1993 Jan;22(1):37-43.


Bindings of glutamate receptor agonists and related modulators were investigated in 10 discrete tissues from gerbil brain using a biochemical technique.
2(0,0,0,2) Details
2881601 Olsen RW, Szamraj O, Houser CR: [3H] AMPA binding to glutamate receptor subpopulations in rat brain. . Brain Res. 1987 Feb 3;402(2):243-54.

1(0,0,0,1) Details
2547898 Ross SM, Roy DN, Spencer PS: Beta-N-oxalylamino-L-alanine action on glutamate receptors. . J Neurochem. 1989 Sep;53(3):710-5.


The present study demonstrates the stereospecific action of L-BOAA on glutamate receptor binding in whole mouse brain synaptic membranes.
1(0,0,0,1) Details
8640342 Hawkins LM, Beaver KM, Jane DE, Taylor PM, Sunter DC, Roberts PJ: Characterization of the pharmacology and regional distribution of (S)-[3H]-5-fluorowillardiine binding in rat brain. Br J Pharmacol. 1995 Oct;116(3):2033-9.

Specific binding represented greater than 80% of the total binding and was increased by 10% in the presence of 100mM potassium thiocyanate (KSCN). 2.
In contrast, both N-methyl-D-aspartate (NMDA) and the metabotropic glutamate receptor agonist, (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD) were inactive. 4.
1(0,0,0,1) Details