Protein Information

Name ecto ATPase
Synonyms CD39 antigen like 1; CD39 like 1; CD39L1; ENTPD 2; ENTPD2; ENTPD2 protein; Ecto ATPase; Ectonucleoside triphosphate diphosphohydrolase 2…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
12209331 dos Passos Lemos A, de Sa Pinheiro AA, de Berredo-Pinho M, Fonseca de Souza L, Motta CM, de Souza W, Meyer-Fernandes R: Ectonucleotide diphosphohydrolase activity in Crithidia deanei. Parasitol Res. 2002 Oct;88(10):905-11. Epub 2002 Jun 19.

This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, vanadate, molybdate, sodium fluoride and tartrate.
35(0,1,1,5) Details
11051109 Meyer-Fernandes JR, Lanz-Mendoza H, Gondim KC, Willott E, Wells MA: Ectonucleotide diphosphohydrolase activities in hemocytes of larval Manduca sexta. Arch Biochem Biophys. 2000 Oct 1;382(1):152-9.

These ecto-ATPase activities were insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A1, ouabain, furosemide, vanadate, sodium fluoride, tartrate, and levamizole.
34(0,1,1,4) Details
18761612 Matin A, Khan NA: Demonstration and partial characterization of ecto-ATPase in Balamuthia mandrillaris and its possible role in the host-cell interactions. Lett Appl Microbiol. 2008 Oct;47(4):348-54.

This ecto-ATPase was insensitive to ouabain, levamisole, sodium azide and sodium orthovanadate but stimulated by MgCl2.
12(0,0,1,7) Details
17095758 Fausther M, Lecka J, Kukulski F, Levesque SA, Pelletier J, Zimmermann H, Dranoff JA, Sevigny J: Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8. Am J Physiol Gastrointest Liver Physiol. 2007 Mar;292(3):G785-95. Epub 2006 Nov 9.

6(0,0,0,6) Details
10639740 Zinchuk VS, Okada T, Kobayashi T: Ecto-ATPase activity in the rat cardiac muscle: biochemical characteristics and histocytochemical localization. Cell Tissue Res. 1999 Dec;298(3):499-509.

In controls, enzyme activity was abolished by diethyl pyrocarbonate and slightly stimulated by digitonin and concanavalin A, whereas sodium orthovanadate, N-ethylmaleimide, and sodium azide yielded no effect.
4(0,0,0,4) Details
15507403 Ghildyal P, Manchanda R: Effects of cooling and ARL 67156 on synaptic ecto-ATPase activity in guinea pig and mouse vas deferens. Auton Neurosci. 2004 Sep 30;115(1-2):28-34.

ATP hydrolysis in both species was found to be insensitive to ouabain (100 microM), sodium azide (1 mM), sodium vanadate (100 microM) and beta-glycerophosphate (10 mM) and was also found to depend on Ca2+ and Mg2+.
3(0,0,0,3) Details
18201730 Rucker B, Almeida ME, Libermann TA, Zerbini LF, Wink MR, Sarkis JJ: E-NTPDases and ecto-5'-nucleotidase expression profile in rat heart left ventricle and the extracellular nucleotide hydrolysis by their nerve terminal endings. Life Sci. 2008 Feb 27;82(9-10):477-86. Epub 2008 Jan 16.

RT-PCR analysis from left ventricle tissue demonstrate different levels of expression of Entpd1 (Cd39), Entpd2 (Cd39L1), Entpd3 (Cd39L3), Entpd5 (Cd39L4) Entpd6, (Cd39L2) and 5'-NT/CD73.
Oligomycin (2 microg/mL) and sodium azide (0.1 mM), both mitochondrial ATPase inhibitors, inhibit only the ATP hydrolysis.
2(0,0,0,2) Details
7522443 Yagi K, Nishino I, Eguchi M, Kitagawa M, Miura Y, Mizoguchi T: Involvement of ecto-ATPase as an ATP receptor in the stimulatory effect of extracellular ATP on NO release in bovine aorta endothelial cells. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1237-43.


Inhibitors of ecto-ATPase, NaN3 and Ap5A, significantly inhibited the stimulation, while an inhibitor of P2Y-purinoceptor and ecto-ATPase, RB2, completely suppressed it.
2(0,0,0,2) Details
14629005 Alves-Ferreira M, Dutra PM, Lopes AH, Ferreira-Pereira A, Scofano HM, Meyer-Fernandes JR: Magnesium-dependent ecto-ATP diphosphohydrolase activity in Herpetomonas muscarum muscarum. Curr Microbiol. 2003 Oct;47(4):265-71.

The ecto-ATPase activity was insensitive to oligomycin and sodium azide, two inhibitors of mitochondrial Mg-ATPase, bafilomycin A1, a V-ATPase inhibitor, ouabain, a Na (+)+K+-ATPase inhibitor and to levamizole, an inhibitor of alkaline phosphatase.
The ecto-ATPase activity was insensitive to oligomycin and sodium azide, two inhibitors of mitochondrial Mg-ATPase, bafilomycin A1, a V-ATPase inhibitor, ouabain, a Na (+)+K+-ATPase inhibitor and to levamizole, an inhibitor of alkaline phosphatase.
2(0,0,0,2) Details
17169379 Buffon A, Ribeiro VB, Wink MR, Casali EA, Sarkis JJ: Nucleotide metabolizing ecto-enzymes in Walker 256 tumor cells: molecular identification, kinetic characterization and biochemical properties. Life Sci. 2007 Feb 13;80(10):950-8. Epub 2006 Nov 22.

A significant inhibition of ATP and ADP hydrolysis was observed in the presence of high concentrations of sodium azide and 0.5 mM of Gadolinium chloride.
Using RT-PCR analysis we identified the mRNA of two members of the ecto-nucleoside triphosphate diphosphohydrolase family (NTPDase 2 and 5) and a 5'-nucleotidase.
1(0,0,0,1) Details
19922813 Rosemberg DB, Rico EP, Langoni AS, Spinelli JT, Pereira TC, Dias RD, Souza DO, Bonan CD, Bogo MR: NTPDase family in zebrafish: Nucleotide hydrolysis, molecular identification and gene expression profiles in brain, liver and heart. Comp Biochem Physiol B Biochem Mol Biol. 2010 Mar;155(3):230-40. Epub 2009 Nov 14.

Sodium azide (20mM), ARL 67156 (300 microM) and Suramin (300 microM) differently altered the nucleotide hydrolysis in zebrafish tissues, suggesting the contribution of distinct NTPDase activities.
Homology-based searches identified the presence of NTPDase1-6 and NTPDase8 orthologs and the phylogeny also grouped three NTPDase2 and two NTPDase5 paralogs.
1(0,0,0,1) Details
10231536 Smith TM, Lewis Carl SA, Kirley TL: Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase. Biochemistry. 1999 May 4;38(18):5849-57.


In addition, the double mutant is substantially less sensitive to inhibition by azide, a more potent inhibitor of ecto-apyrases than ecto-ATPases.
1(0,0,0,1) Details
16380977 Entringer PF, Gondim KC, Meyer-Fernandes JR: Ecto-nucleotidase activities in the fat body of Rhodnius prolixus. Arch Insect Biochem Physiol. 2006 Jan;61(1):1-9.


Both ecto-nucleotidase activities were insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin, ouabain, vanadate, molybdate, sodium fluoride, levamizole, tartrate, p-NPP, sodium phosphate, and suramin.
0(0,0,0,0) Details
8023897 Trinkle-Mulcahy L, Siegman MJ, Butler TM: Metabolic characteristics of alpha-toxin-permeabilized smooth muscle. . Am J Physiol. 1994 Jun;266(6 Pt 1):C1673-83.

Treatment of the intact muscle with the ectonucleotidase inhibitor 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) followed by alpha-toxin permeabilization and inclusion of sodium azide in subsequent solutions reduces the ecto-ATPase by approximately 70%.
82(1,1,1,2) Details
11750998 Jesus JB, Lopes AH, Meyer-Fernandes JR: Characterization of an ecto-ATPase of Tritrichomonas foetus. Vet Parasitol. 2002 Jan 3;103(1-2):29-42.

The Mg (2+)-dependent ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, furosemide, vanadate, molybdate, sodium fluoride and levamizole.
37(0,1,1,7) Details
11414680 Berredo-Pinho M, Peres-Sampaio CE, Chrispim PP, Belmont-Firpo R, Lemos AP, Martiny A, Vannier-Santos MA, Meyer-Fernandes JR: A Mg-dependent ecto-ATPase in Leishmania amazonensis and its possible role in adenosine acquisition and virulence. Arch Biochem Biophys. 2001 Jul 1;391(1):16-24.

This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, furosemide, vanadate, molybdate, sodium fluoride, tartrate, and levamizole.
36(0,1,1,6) Details
7761265 Ziganshin AU, Ziganshina LE, King BE, Burnstock G: Characteristics of ecto-ATPase of Xenopus oocytes and the inhibitory actions of suramin on ATP breakdown. Pflugers Arch. 1995 Jan;429(3):412-8.

Ecto-ATPase activity was unaffected by ouabain (100 microM), sodium azide (100 microM), and oligomycin (5 micrograms/ml) (as inhibitors of endo-ATPases) and beta-glycerophosphate (10 mM) and p-nitrophenyl phosphate (10 mM) (as inhibitors of non-specific alkaline phosphatase).
36(0,1,1,6) Details
16289087 Fonseca FV, Fonseca de Souza AL, Mariano AC, Entringer PF, Gondim KC, Meyer-Fernandes JR: Trypanosoma rangeli: characterization of a Mg-dependent ecto ATP-diphosphohydrolase activity. Exp Parasitol. 2006 Feb;112(2):76-84. Epub 2005 Nov 9.

This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A1, ouabain, furosemide, vanadate, molybdate, sodium fluoride, tartrate, and levamizole.
36(0,1,1,6) Details