Protein Information

Name Myosin
Synonyms Myosin

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
8894285 Wymer CL, Fisher DD, Moore RC, Cyr RJ: Elucidating the mechanism of cortical microtubule reorientation in plant cells. Cell Motil Cytoskeleton. 1996;35(2):162-73.


Sodium azide (1 mM) and sodium cyanide (1 mM) can prevent reorientation, indicating that cellular energy is required for this event but this energy is not used by the actin-myosin system because the microfilament-disrupting drug cytochalasin D (50 microM) did not affect reorientation.
0(0,0,0,0) Details
7498732 Patterson B, Spudich JA: A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium. Genetics. 1995 Jun;140(2):505-15.

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10198341 Ma TY, Nguyen D, Bui V, Nguyen H, Hoa N: Ethanol modulation of intestinal epithelial tight junction barrier. Am J Physiol. 1999 Apr;276(4 Pt 1):G965-74.

Ethanol produced a disassembly and displacement of perijunctional actin and myosin filaments from the perijunctional areas.
Consistent with these findings, metabolic inhibitors sodium azide and 2,4-dinitrophenol significantly prevented ethanol-induced increase in Caco-2 TJ permeability, whereas cycloheximide or actinomycin D had no effect.
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15701621 Ma TY, Boivin MA, Ye D, Pedram A, Said HM: Mechanism of TNF-{alpha} modulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. Am J Physiol Gastrointest Liver Physiol. 2005 Mar;288(3):G422-30.


Moreover, inhibitors of MLCK, Mg (2+)-myosin ATPase, and metabolic energy prevented the TNF-alpha increase in Caco-2 TJ permeability, suggesting that the increase in MLCK activity was required for the TNF-alpha-induced opening of the Caco-2 TJ barrier.
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11832505 Maselli A, Laevsky G, Knecht DA: Kinetics of binding, uptake and degradation of live fluorescent (DsRed) bacteria by Dictyostelium discoideum. Microbiology. 2002 Feb;148(Pt 2):413-20.

To distinguish internalized bacteria from those bound to the surface, an assay was developed in which sodium azide was used to release surface-bound particles.
Surprisingly, surface particle release appears to be independent of myosin II function.
1(0,0,0,1) Details
11090349 Soong RK, Bachand GD, Neves HP, Olkhovets AG, Craighead HG, Montemagno CD: Powering an inorganic nanodevice with a biomolecular motor. Science. 2000 Nov 24;290(5496):1555-8.

Biomolecular motors such as F1-adenosine triphosphate synthase (F1-ATPase) and myosin are similar in size, and they generate forces compatible with currently producible nanoengineered structures.
Rotation of the nanopropeller was initiated with 2 mM adenosine triphosphate and inhibited by sodium azide.
1(0,0,0,1) Details
11195789 Kositprapa C, Zhang B, Berger S, Canty JM Jr, Lee TC: Calpain-mediated proteolytic cleavage of troponin I induced by hypoxia or metabolic inhibition in cultured neonatal cardiomyocytes. Mol Cell Biochem. 2000 Nov;214(1-2):47-55.


Other myofilament proteins such as troponin T, myosin heavy chain, and actin appeared to remain largely intact. p53-mediated cell injury exhibited proteolysis of the caspase protein substrate lamin B without appreciable breakdown of TnI.
1(0,0,0,1) Details
15326353 Pelling AE, Sehati S, Gralla EB, Valentine JS, Gimzewski JK: Local nanomechanical motion of the cell wall of Saccharomyces cerevisiae. Science. 2004 Aug 20;305(5687):1147-50.


From the strong frequency dependence on temperature, we derive an activation energy of 58 kJ/mol, which is consistent with the cell's metabolism involving molecular motors such as kinesin, dynein, and myosin.
1(0,0,0,1) Details
9398078 Kay L, Li Z, Mericskay M, Olivares J, Tranqui L, Fontaine E, Tiivel T, Sikk P, Kaambre T, Samuel JL, Rappaport L, Usson Y, Leverve X, Paulin D, Saks VA: Study of regulation of mitochondrial respiration in vivo. Biochim Biophys Acta. 1997 Nov 10;1322(1):41-59.


Also, cardiomyocytes were isolated from the normal rat heart, permeabilized by saponin and the "ghost" (phantom) cardiomyocytes were produced by extraction of myosin with 800 mM KCl.
1(0,0,0,1) Details