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Cho S, Liu D, Fairman D, Li P, Jenkins L, McGonigle P, Wood A: Spatiotemporal evidence of apoptosis-mediated ischemic injury in organotypic hippocampal slice cultures. Neurochem Int. 2004 Jul;45(1):117-27.
Neuronal death was first evident in the CA1 region 24 h after the injury as assessed by propidium iodide (PI) labeling, and continued to extend to the CA3/4 region up to 72 h.
In order to ascertain the existence of the synaptic pool of glutamate, sister cultures were treated with sodium azide. |
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