| Name | ATPase |
|---|---|
| Synonyms | ATP7A; MK; ATPase; Cation transporting ATPase; ATP7A protein; ATPase Cu(2+) transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1… |
| Name | sodium azide |
|---|---|
| CAS | sodium azide |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 14577168 | Slinchenko NM, Chernysh IH, Kosterin SO: [Utilization of purified myometrium cell plasma membrane Ca2+, Mg2+-ATPase for comparative estimation of the efficiency of energy-dependent Ca2+-transport inhibitors]. Ukr Biokhim Zh. 2003 Mar-Apr;75(2):33-8. Sodium azide (10 mM) blocking completely Ca (2+)-transport in mitochondria inhibited activity of the plasma membrane Ca (2+)-transporting ATPase by 14%. |
83(1,1,1,3) | Details |
| 10368660 | Candussio L, Decorti G, Crivellato E, Rosati AM, Traversa U, Klugmann FB: Transport of doxorubicin in mast cell granules and the effect of the antagonist Anticancer Res. 1999 Mar-Apr;19(2A):1101-8. Similar results were obtained with the metabolic inhibitors metavanadate, N-ethylmaleimide, and sodium azide, whereas ouabain, an inhibitor of - ATPase, was without effect. |
81(1,1,1,1) | Details |
| 7548149 | Elzainy TA, Ali TH: Participation of a -translocating plasma membrane ATPase, acid phosphatase and alkaline phosphatase in ATP degradation by Aspergillus niger extracts. Biochim Biophys Acta. 1995 Oct 4;1239(1):91-7. It was neither inhibited by sodium azide nor by but inhibited by orthovanadate, DES, DCCD, Mg2+ and Pi. |
3(0,0,0,3) | Details |
| 7902705 | Garrigos M, Belehradek J Jr, Mir LM, Orlowski S: Absence of cooperativity for MgATP and effects on the ATPase activity of P-glycoprotein containing membrane vesicles. Biochem Biophys Res Commun. 1993 Nov 15;196(3):1034-41. |
3(0,0,0,3) | Details |
| 8797850 | Beharry S, Bragg PD: The bound nucleotides of purified bovine mitochondrial ATP synthase. Eur J Biochem. 1996 Aug 15;240(1):165-72. Incubation of the synthase with 250 microM MgATP in the presence of 3 mM sodium azide, an inhibitor of the ATPase, resulted in the transitory formation of a form of the enzyme in which 5-6 nucleotide-binding sites were loaded with ATP and/or ADP, thus showing that the ATP synthase, like the soluble F1 ATPase, contained a minimum of six nucleotide-binding sites. Incubation of the synthase with 250 microM MgATP in the presence of 3 mM sodium azide, an inhibitor of the ATPase, resulted in the transitory formation of a form of the enzyme in which 5-6 nucleotide-binding sites were loaded with ATP and/or ADP, thus showing that the ATP synthase, like the soluble F1 ATPase, contained a minimum of six nucleotide-binding sites. |
3(0,0,0,3) | Details |
| 9792691 | Kihara A, Ito K: Translocation, folding, and stability of the HflKC complex with signal anchor topogenic sequences. J Biol Chem. 1998 Nov 6;273(45):29770-5. In contrast, translocation of these domains was retarded markedly when sodium azide was added to inhibit SecA ATPase and blocked almost completely in secY- or secD-defective mutant cells. |
81(1,1,1,1) | Details |
| 9726993 | Vasilyeva E, Forgac M: Interaction of the clathrin-coated vesicle V-ATPase with ADP and sodium azide. J Biol Chem. 1998 Sep 11;273(37):23823-9. |
37(0,1,1,7) | Details |
| 7750790 | Rohn TT, Hinds TR, Vincenzi FF: Inhibition by activated neutrophils of the Ca2+ pump ATPase of intact red blood cells. Free Radic Biol Med. 1995 Apr;18(4):655-67. The addition of sodium azide, a potent inhibitor of endogenous RBC catalase, enhanced inhibition of the Ca2+ pump ATPase of intact RBCs. |
36(0,1,1,6) | Details |
| 11414680 | Berredo-Pinho M, Peres-Sampaio CE, Chrispim PP, Belmont-Firpo R, Lemos AP, Martiny A, Vannier-Santos MA, Meyer-Fernandes JR: A Mg-dependent ecto-ATPase in Leishmania amazonensis and its possible role in acquisition and virulence. Arch Biochem Biophys. 2001 Jul 1;391(1):16-24. This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, vanadate, and levamizole. |
35(0,1,1,5) | Details |
| 8347662 | Muneyuki E, Makino M, Kamata H, Kagawa Y, Yoshida M, Hirata H: Inhibitory effect of NaN3 on the F0F1 ATPase of submitochondrial particles as related to nucleotide binding. Biochim Biophys Acta. 1993 Aug 16;1144(1):62-8. It was shown that NaN3 inhibited the ATPase activity only in the presence of ATP or ADP and the inhibition proceeded slowly. |
3(0,0,0,3) | Details |
| 8761458 | Vanderheyden N, Benaim G, Docampo R: The role of a H (+)-ATPase in the regulation of cytoplasmic pH in Trypanosoma cruzi epimastigotes. Biochem J. 1996 Aug 15;318 ( Pt 1):103-9. |
3(0,0,0,3) | Details |
| 9226881 | Specht SC, Rodriguez C, Quinones L, Velazquez S: Effect of high ionic strength and inhibitors of H,K-ATPase on the ouabain-sensitive K-p-nitrophenylphosphatase activity in the sea anemone Stichodactyla helianthus. Comp Biochem Physiol B Biochem Mol Biol. 1997 Jun;117(2):217-24. Activity of the sea anemone enzyme was inhibited by 10 microM ammonium vanadate, an inhibitor of P-type ATPase, and not by 2.5 mM sodium azide, an inhibitor of both F-type and V-type ATPase. |
2(0,0,0,2) | Details |
| 19060940 | Kosenko EA, Solomadin IN, Marov NV, Venediktova NI, Pogosian AS, Kaminskii IuG: [Role of glycolysis and antioxidant enzymes in the toxicity of amyloid beta peptide Abeta25-35 to erythrocytes]. Bioorg Khim. 2008 Sep-Oct;34(5):654-60. a Cu,Zn-superoxide dismutase inhibitor, abolishes the toxic effect of Abeta (25-35) to erythrocytes, whereas mercaptosuccinate, a peroxidase inhibitor, and ouabain, a Na+,K+-ATPase inhibitor, promote it. Sodium azide, a catalase inhibitor, did not affect the cell lysis under the action of Abeta (25-35) . |
2(0,0,0,2) | Details |
| 10231536 | Smith TM, Lewis Carl SA, Kirley TL: Mutagenesis of two conserved residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase. Biochemistry. 1999 May 4;38(18):5849-57. A human brain E-type ATPase (HB6 ecto-apyrase) was subjected to site-directed mutagenesis to assess the functional significance of two highly conserved residues (Trp 187 and Trp 459), the only two tryptophans conserved in nearly all E-type ATPases. |
2(0,0,0,2) | Details |
| 11051109 | Meyer-Fernandes JR, Lanz-Mendoza H, Gondim KC, Willott E, Wells MA: Ectonucleotide diphosphohydrolase activities in hemocytes of larval Manduca sexta. Arch Biochem Biophys. 2000 Oct 1;382(1):152-9. These ecto-ATPase activities were insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A1, ouabain, vanadate, and levamizole. |
34(0,1,1,4) | Details |
| 8768517 | Aparicio G, Buche A, Mendez C, Salas JA: Characterization of the ATPase activity of the N-terminal nucleotide binding domain of an ABC transporter involved in oleandomycin secretion by Streptomyces antibioticus. FEMS Microbiol Lett. 1996 Aug 1;141(2-3):157-62. ATPase activity was resistant to specific inhibitors of P-, F-, and V-type ATPase whereas sodium azide and 7-chloro-4-nitrobenzo-2-oxa- (NBD-C1) were strong inhibitors. |
34(0,1,1,4) | Details |
| 11561081 | Lee G, Schlichter L, Bendayan M, Bendayan R: Functional expression of P-glycoprotein in rat brain microglia. J Pharmacol Exp Ther. 2001 Oct;299(1):204-12. Immunocytochemical analysis with the monoclonal antibodies C219, MRK16, and MAB-448 labeled P-gp protein along the plasma membrane and nuclear envelope of MLS-9 cells. [3H] accumulation by monolayers of MLS-9 cells was significantly enhanced in the presence of any of several P-gp inhibitors cyclosporin A, quinidine, PSC 833), protease inhibitors (i.e., saquinavir, indinavir, and ritonavir), and sodium azide, an ATPase inhibitor. |
31(0,1,1,1) | Details |
| 15180339 | Ronaldson PT, Lee G, Dallas S, Bendayan R: Involvement of P-glycoprotein in the transport of saquinavir and indinavir in rat brain microvessel endothelial and microglia cell lines. Pharm Res. 2004 May;21(5):811-8. Cellular accumulation of [14C] saquinavir and [3H] indinavir by RBE4, MLS-9, and CHRC5 monolayers was significantly enhanced in the presence of P-gp inhibitors, HIV-1 protease inhibitors, the ATPase inhibitor sodium azide, and the ATP depleting agent 2',4'-dinitrophenol respectively. [14C] Saquinavir and [3H] indinavir efflux from both cell systems was rapid and significantly reduced in the presence of PSC833. |
31(0,1,1,1) | Details |
| 8531496 | Fitzgerald EM, Djamgoz MB: Sensitivity of valinomycin-based K (+)-selective micro-electrodes to inhibitors of K+ transport. J Neurosci Methods. 1995 Jul;59(2):273-7. The sensitivies of double-barrelled K (+)-selective micro-electrodes (KSMs) employing the low-impedance membrane cocktail based on the neutral K (+)-selective ion carrier valinomycin (Fluka, Cocktail B 60398) to the following 3 different classes of inhibitors of K+ transport were measured: (1) general metabolic inhibitors (dinitrophenol, sodium azide, rotenone, dicyclohexylcarbodiimide, salicylhydroxamic acid); (2) P-type ATPase inhibitors (vanadate, ouabain, amiloride, SCH 28080); and (3) anion-dependent K+ transport inhibitors (bumetanide, 4-acetamide-4-isothiocyanostilbene-2,2-disulphonic acid). |
31(0,1,1,1) | Details |
| 16327018 | Qamirani E, Razavi HM, Wu X, Davis MJ, Kuo L, Hein TW: Sodium azide dilates coronary arterioles via activation of inward rectifier K+ channels and Na+-K+-ATPase. Am J Physiol Heart Circ Physiol. 2006 Apr;290(4):H1617-23. Epub 2005 Dec 3. However, the vasomotor action of NaN (3) was significantly attenuated in a similar manner by the inward rectifier K (+) (K (IR)) channel inhibitor Ba (2+), the Na (+)-K (+) ATPase inhibitor ouabain, or the guanylyl cyclase inhibitor 1H-[1,2,4] oxadiazolo [4,3,-a] quinoxalin-1-one (ODQ). |
2(0,0,0,2) | Details |
| 14616079 | Golovastov VV, Nesmeyanova MA: Effect of membrane phospholipid composition and charge of the signal peptide of Escherichia coli alkaline phosphatase on efficiency of its secretion. Biochemistry. 2003 Oct;68(10):1089-96. The secretion of the Escherichia coli alkaline phosphatase with a different charge of signal peptide due to replacement of positively charged (-20) has been studied depending on the phospholipid composition of the membranes and the activity of the translocational ATPase--protein SecA. If SecA is inactivated by sodium azide, then the dependence of secretion on anionic phospholipids increases; on the contrary, higher content of anionic phospholipids (in the absence of phosphatidylethanolamine) decreases the dependence of secretion on the SecA activity. |
2(0,0,0,2) | Details |
| 7486907 | Belli WA, Fryklund J: Partial characterization and effect of on ATPase activity in Helicobacter pylori by using permeabilized cells. Antimicrob Agents Chemother. 1995 Aug;39(8):1717-20. The ATPase activities in these cells were most susceptible to sodium azide, fluoroaluminate, and dicyclohexylcarbodiimide, which are typical inhibitors of F ATPases. |
34(0,1,1,4) | Details |
| 11113555 | Benrezzak O, Grondin G, Proulx J, Rousseau E, D'Orleans-Juste P, Beaudoin AR: Characterization and immunohistochemical localization of nucleoside diphosphohydrolase (NTPDase) in pig adrenal glands (presence of a non-sedimentable isoform). Biochim Biophys Acta. 2000 Dec 15;1524(2-3):94-101. Both ATPase and ADPase activities were similarly inhibited by sodium azide. |
33(0,1,1,3) | Details |
| 10856430 | Nielsen D, Maare C, Eriksen J, Litman T, Friche E, Skovsgaard T: Characterisation of multidrug-resistant Ehrlich ascites tumour cells selected in vivo for resistance to etoposide. Biochem Pharmacol. 2000 Aug 1;60(3):353-61. Compared with that of sensitive cells, the ATPase activity of plasma membrane vesicles prepared from EHR2/VP16 cells was very low. Deprivation of energy by addition of sodium azide increased the accumulation of both drugs to the level of sensitive cells. |
2(0,0,0,2) | Details |
| 8720695 | Vrbjar N, Dzurba A, Ziegelhoffer A: Enzyme kinetics and the activation energy of Mg-ATPase in cardiac sarcolemma: ADP as an alternative substrate. Gen Physiol Biophys. 1995 Aug;14(4):313-21. |
2(0,0,0,2) | Details |
| 16289087 | Fonseca FV, Fonseca de Souza AL, Mariano AC, Entringer PF, Gondim KC, Meyer-Fernandes JR: Trypanosoma rangeli: characterization of a Mg-dependent ecto ATP-diphosphohydrolase activity. Exp Parasitol. 2006 Feb;112(2):76-84. Epub 2005 Nov 9. This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A1, ouabain, vanadate, and levamizole. |
33(0,1,1,3) | Details |
| 11090349 | Soong RK, Bachand GD, Neves HP, Olkhovets AG, Craighead HG, Montemagno CD: Powering an inorganic nanodevice with a biomolecular motor. Science. 2000 Nov 24;290(5496):1555-8. Biomolecular motors such as F1- synthase (F1-ATPase) and myosin are similar in size, and they generate forces compatible with currently producible nanoengineered structures. Rotation of the nanopropeller was initiated with 2 mM and inhibited by sodium azide. |
2(0,0,0,2) | Details |
| 8673003 | Battastini AM, Oliveira EM, Moreira CM, Bonan CD, Sarkis JJ, Dias RD: Solubilization and characterization of an ATP diphosphohydrolase (EC 3.6.1.5) from rat brain synaptic plasma membranes. Biochem Mol Biol Int. 1995 Oct;37(2):209-19. Treatment of the synaptic plasma membrane from rat brain with 0.5% digitonin solubilizes 80% of the proteins and 50% and 60% of ATPase and ADPase, respectively, with the following characteristics: stimulation by Ca2+ in the millimolar range, insensitivity to ATPase inhibitors (ouabain, olygomicyn, orthovanadate), inhibition with sodium azide and NEM and broad substrate specificity for the hydrolysis of nucleoside di- and |
33(0,1,1,3) | Details |
| 12223778 | Helguera G, Beauge L: Phosphoryl Group Exchange between ATP and ADP Catalyzed by H+-ATPase from Oat Roots. Plant Physiol. 1997 Aug;114(4):1397-1403. At an alkaline pH the reaction was not affected by sodium azide and carbonyl p-trifluometoxyphenyl-hydrazone, had a biphasic response to Mg2+ (maximal at 513 [mu] m), and was insensitive to ionic strength. |
2(0,0,0,2) | Details |
| 11750998 | Jesus JB, Lopes AH, Meyer-Fernandes JR: Characterization of an ecto-ATPase of Tritrichomonas foetus. Vet Parasitol. 2002 Jan 3;103(1-2):29-42. The Mg (2+)-dependent ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, vanadate, and levamizole. |
33(0,1,1,3) | Details |
| 12209331 | dos Passos Lemos A, de Sa Pinheiro AA, de Berredo-Pinho M, Fonseca de Souza L, Motta CM, de Souza W, Meyer-Fernandes R: Ectonucleotide diphosphohydrolase activity in Crithidia deanei. Parasitol Res. 2002 Oct;88(10):905-11. Epub 2002 Jun 19. This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, vanadate, and |
33(0,1,1,3) | Details |
| 17169379 | Buffon A, Ribeiro VB, Wink MR, Casali EA, Sarkis JJ: Nucleotide metabolizing ecto-enzymes in Walker 256 tumor cells: molecular identification, kinetic characterization and biochemical properties. Life Sci. 2007 Feb 13;80(10):950-8. Epub 2006 Nov 22. A significant inhibition of ATP and ADP hydrolysis was observed in the presence of high concentrations of sodium azide and 0.5 mM of Gadolinium These activities were insensitive to ATPase, kinase and alkaline phosphatase classical inhibitors. |
1(0,0,0,1) | Details |
| 7948786 | Bendayan R, Lo B, Silverman M: Characterization of cimetidine transport in LLCPK1 cells. J Am Soc Nephrol. 1994 Jul;5(1):75-84. Furthermore, the activity of both the Na (+)-H+ exchanger and H (+)-ATPase acted to dissipate the cell--> lumen H+ gradient, thereby decreasing net cimetidine transport. |
1(0,0,0,1) | Details |
| 9972316 | Podsiadlowski L, Matha V, Vilcinskas A: Detection of a P-glycoprotein related pump in Chironomus larvae and its inhibition by and cyclosporin A. Comp Biochem Physiol B Biochem Mol Biol. 1998 Dec;121(4):443-50. The in vitro ATPase activity of homogenate fractions increased in the presence of typical P-glycoprotein substrates (vinblastine, actinomycin D or ivermectin). This increase was unaffected by inhibitors of other membrane ATPases (sodium azide, EGTA, ouabain), but sensitive to vanadate, cyclosporin A and which inhibit mammalian P-glycoprotein mediated ATP-consumption. |
1(0,0,0,1) | Details |
| 14872239 | Karu TI, Pyatibrat LV, Kalendo GS: Photobiological modulation of cell attachment via cytochrome c oxidase. . Photochem Photobiol Sci. 2004 Feb;3(2):211-6. Epub 2003 Nov 12. Treating the cell suspension with ouabain (a Na (+), K (+)-ATPase inhibitor), amiloride (an inhibitor of N (+)/H (+) exchangers), or sodium azide (a cytochrome c oxidase inhibitor) prior to irradiation significantly modifies the action spectrum of cell attachment enhancement. |
32(0,1,1,2) | Details |
| 11403767 | de Aguiar Matos JA, Borges FP, Tasca T, Bogo MR, De Carli GA, da Graca Fauth M, Dias RD, Bonan CD: Characterisation of an ATP diphosphohydrolase (Apyrase, EC 3.6.1.5) activity in Trichomonas vaginalis. Int J Parasitol. 2001 Jun;31(8):770-5. It is Ca (2+)-dependent and is insensitive to classical ATPase inhibitors, such as ouabain (1 mM), N-ethylmaleimide (0.1 mM), orthovanadate (0.1 mM) and sodium azide (5 mM). |
32(0,1,1,2) | Details |
| 9837890 | Weber J, Senior AE: Effects of the inhibitors azide, dicyclohexylcarbodiimide, and aurovertin on nucleotide binding to the three F1-ATPase catalytic sites measured using specific probes. J Biol Chem. 1998 Dec 11;273(50):33210-5. |
2(0,0,0,2) | Details |
| 10224124 | Saint-Pol A, Codogno P, Moore SE: Cytosol-to-lysosome transport of free polymannose-type oligosaccharides. J Biol Chem. 1999 May 7;274(19):13547-55. Transport was inhibited strongly by NEM and weakly by vanadate but not by sodium azide, and, in addition, the sugar transport inhibitors phloridzin, and cytochalasin B were without effect on transport. Saturable high affinity transport (Kuptake, 22.3 microM, Vmax, 7.1 fmol/min/unit of beta-hexosaminidase) was dependent upon the hydrolysis of ATP but independent of vacuolar H+/ATPase activity. |
1(0,0,0,1) | Details |
| 10681547 | Sevigny J, Robson SC, Waelkens E, Csizmadia E, Smith RN, Lemmens R: Identification and characterization of a novel hepatic canalicular ATP diphosphohydrolase. J Biol Chem. 2000 Feb 25;275(8):5640-7. NH (2)-terminal amino acid sequencing revealed near 50% identity with rat liver lysosomal (Ca (2+)-Mg (2+))-ATPase. Biochemical activity was unaffected by sodium azide or other inhibitors of ATPases. |
1(0,0,0,1) | Details |
| 16808345 | Akopian M, Poladian A, Bagramian K: [Energy transformation coupled to oxidation during anaerobic fermentation]. Biofizika. 2006 May-Jun;51(3):466-71. ATP synthesis was suppressed by H+ -ATPase inhibitors N,N'-dicyclohexylcarbodiimide (DCCD) and sodium azide as well as by the protonophore carbonyl -m-chlorophenyhydrazone (CCCP). |
31(0,1,1,1) | Details |
| 12914800 | Mnatsakanyan N, Poladian A, Bagramyan K, Trchounian A: The number of accessible SH-groups in Escherichia coli membrane vesicles is increased by ATP or by Biochem Biophys Res Commun. 2003 Aug 29;308(3):655-9. The increase was inhibited by treatment with N-ethylmaleimide or the presence of the F (0) F (1)-ATPase inhibitors N,N (')-dicyclohexylcarbodiimide or sodium azide. |
31(0,1,1,1) | Details |
| 12899930 | Rico EP, Senger MR, Fauth Mda G, Dias RD, Bogo MR, Bonan CD: ATP and ADP hydrolysis in brain membranes of zebrafish (Danio rerio). Life Sci. 2003 Sep 5;73(16):2071-82. It was able to hydrolyze and pyrimidine nucleosides 5'-di and triphosphates, being insensitive to classical ATPase inhibitors, such as ouabain (1 mM), N-ethylmaleimide (0.1 mM), orthovanadate (0.1 mM) and sodium azide (0.1 mM). |
31(0,1,1,1) | Details |
| 8179599 | Matsuda C, Muneyuki E, Endo H, Yoshida M, Kagawa Y: Comparison of the ATPase activities of bovine heart and liver mitochondrial ATP synthases with different tissue-specific gamma subunit isoforms. Biochem Biophys Res Commun. 1994 Apr 29;200(2):671-8. Moreover sodium azide inhibited the two equally. |
2(0,0,0,2) | Details |
| 12893836 | Dallas S, Zhu X, Baruchel S, Schlichter L, Bendayan R: Functional expression of the multidrug resistance protein 1 in microglia. J Pharmacol Exp Ther. 2003 Oct;307(1):282-90. Epub 2003 Jul 31. Vincristine accumulation by monolayers of MLS-9 cells increased significantly in the presence of several well established MRP1 inhibitors (MK571, sulfinpyrazone, probenecid, and indomethacin), protease inhibitors, or the ATPase inhibitor sodium azide. |
31(0,1,1,1) | Details |
| 10074074 | Leloup L, Driessen AJ, Freudl R, Chambert R, Petit-Glatron MF: Differential dependence of levansucrase and alpha-amylase secretion on SecA (Div) during the exponential phase of growth of Bacillus subtilis. J Bacteriol. 1999 Mar;181(6):1820-6. SecA, the translocation ATPase of the preprotein translocase, accounts for 0.25% of the total protein in a degU32 (Hy) Bacillus subtilis strain in logarithmic phase. The level of SecA was reduced in the presence of sodium azide and in the div341 thermosensitive mutant at nonpermissive temperatures. |
1(0,0,0,1) | Details |
| 15967381 | Grossini E, Avanzi G, Gallicchio M, Molinari C, Vacca G, Bellomo G: Regulation of Ca2+ movements by cyclovirobuxine D in ECV304 endothelial cells. Pharmacol Res. 2005 Aug;52(2):154-61. In Fura-2/loaded ECV304 endothelial cells cyclovirobuxine D promoted a transient increase in cytosolic free Ca2+ originating from both an intracellular pool sensitive to the endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin and the extracellular space. In cells treated with digitonin, cyclovirobuxine D did not promote any Ca2+ release from the intracellular stores even at high concentrations and in the absence or presence of thapsigargin or sodium azide, the inhibitors of the endoplasmic reticular or mitochondrial Ca2+ uptake. |
1(0,0,0,1) | Details |
| 16380977 | Entringer PF, Gondim KC, Meyer-Fernandes JR: Ecto-nucleotidase activities in the fat body of Rhodnius prolixus. Arch Insect Biochem Physiol. 2006 Jan;61(1):1-9. Both ecto-nucleotidase activities were insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin, ouabain, vanadate, levamizole, p-NPP, and suramin. |
31(0,1,1,1) | Details |
| 11577722 | Nakamura M, Kuroiwa N, Kono Y, Takatsuki A: Characterization of clofibrate-induced retrograde Golgi membrane movement to the endoplasmic reticulum: clofibrate distinguishes the Golgi from the trans Golgi network. Biosci Biotechnol Biochem. 2001 Aug;65(8):1812-23. Clofibrate-induced retrograde Golgi membrane movement was blocked or retarded when NRK cells were treated with sodium azide/2-deoxyglucose, nocodazole, taxol, and destruxin B, indicating that it depends on energy, and the dynamic state of microtubules, and being acidic or vacuolar-type ATPase function. |
31(0,1,1,1) | Details |
| 15143516 | Tuhai VA, Danylovych IuV: [Enzymes and processes of activation of the endometrium stromal cells] . Ukr Biokhim Zh. 2003 Nov-Dec;75(6):40-6. Common Mg2+, Ca (2+)-ATPase activity of compounds of 36 +/- 2 mumol Pi/mg protein/hour, is depressed by sodium azide and thapsigargine, that specifies presence of an investigated enzyme in mitochondria and endoplasmic reticulum of investigated cells. |
7(0,0,1,2) | Details |
| 8026510 | Mueller DM, Indyk V, McGill L: ATPase kinetics for wild-type Saccharomyces cerevisiae F1-ATPase and F1-ATPase with the beta-subunit Thr197--> Ser mutation. Eur J Biochem. 1994 Jun 15;222(3):991-9. |
7(0,0,0,7) | Details |
| 10510290 | Benrezzak O, Grondin G, Sevigny J, Gendron FP, Rousseau E, D'Orleans-Juste P, Beaudoin AR: Identification and immunolocalization of two isoforms of ATP-diphosphohydrolase (ATPDase) in the pig immune system. Arch Biochem Biophys. 1999 Oct 15;370(2):314-22. The parallel decrease in ATPase and ADPase activities, in the presence of sodium azide, indicated that an ATP diphosphohydrolase (ATPDase) was responsible for these activities. |
7(0,0,1,2) | Details |
| 8023897 | Trinkle-Mulcahy L, Siegman MJ, Butler TM: Metabolic characteristics of alpha-toxin-permeabilized smooth muscle. . Am J Physiol. 1994 Jun;266(6 Pt 1):C1673-83. Rabbit portal veins were permeabilized using Staphylococcus aureus alpha-toxin, and adenosinetriphosphatase (ATPase) was measured as the formation of [3H] ADP, [3H] AMP, and [3H] from [3H] ATP in the solution bathing the muscle. Treatment of the intact muscle with the ectonucleotidase inhibitor 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) followed by alpha-toxin permeabilization and inclusion of sodium azide in subsequent solutions reduces the ecto-ATPase by approximately 70%. |
6(0,0,0,6) | Details |
| 10580101 | Machida K, Tanaka T: -induced generation of reactive species dependent on mitochondrial transmembrane potential hyperpolarization mediated by F (0) F (1)-ATPase in yeast. FEBS Lett. 1999 Nov 26;462(1-2):108-12. The FOH-induced events were coordinately abolished with the F (1)-ATPase inhibitor sodium azide as well as the F (0) F (1)-ATPase inhibitor oligomycin, suggesting the dependence of ROS generation on mtDeltaPsi hyperpolarization mediated by the pumping function of F (0) F (1)-ATPase as a result of hydrolysis. |
89(1,1,2,4) | Details |
| 8918233 | Blanco J, Coque JJ, Martin JF: Characterization of the secA gene of Streptomyces lividans encoding a protein translocase which complements and Escherichia coli mutant defective in the ATPase activity of SecA. Gene. 1996 Oct 17;176(1-2):61-5. The secA gene of S. lividans restored sensitivity to sodium azide in E. coli SecA4 (AzR) a mutant with an azide-resistant (ATPase defective) SecA protein. |
7(0,0,1,2) | Details |
| 11278748 | Kawasaki-Nishi S, Nishi T, Forgac M: Yeast V-ATPase complexes containing different isoforms of the 100-kDa a-subunit differ in coupling efficiency and in vivo dissociation. J Biol Chem. 2001 May 25;276(21):17941-8. Epub 2001 Mar 2. Both exhibited a K (m) for ATP of about 250 microm, and both showed resistance to sodium azide and vanadate and sensitivity to nanomolar concentrations of concanamycin A. |
5(0,0,0,5) | Details |
| 7894702 | Nakane A, Takamatsu H, Oguro A, Sadaie Y, Nakamura K, Yamane K: Acquisition of azide-resistance by elevated SecA ATPase activity confers azide-resistance upon cell growth and protein translocation in Bacillus subtilis. Microbiology. 1995 Jan;141 ( Pt 1):113-21. The elevated ATPase activity of the SecA mutants was reduced upon the addition of 1.5 mM sodium azide by only 5-10% as compared with 40% for that of the wild-type. |
89(1,1,2,4) | Details |
| 11567022 | Sarin J, Aggarwal S, Chaba R, Varshney GC, Chakraborti PK: B-subunit of -specific transporter from Mycobacterium tuberculosis is a thermostable ATPase. J Biol Chem. 2001 Nov 30;276(48):44590-7. Epub 2001 Sep 20. The classical ATPase inhibitors like sodium azide, vanadate, and N-ethylmaleimide were without any effect but an ATP analogue, 5'-p-fluorosulfonylbenzoyl inhibited the ATPase function of the recombinant protein with a K (i) of approximately 0.40 mm. |
87(1,1,1,7) | Details |
| 7667394 | Beil W, Birkholz C, Wagner S, Sewing KF: Bismuth subcitrate and inhibit Helicobacter pyloriF1-ATPase. . Pharmacology. 1995 May;50(5):333-7. Mg (2+)-dependent ATPase activity was inhibited by sodium azide and the monovalent cations K+ and Na+, but not by oligomycin, dicyclohexylcarbodiimide, ouabain, or SCH 28080. |
5(0,0,0,5) | Details |
| 12804571 | Bagramyan K, Mnatsakanyan N, Trchounian A: increases the F0F1-ATPase activity in Escherichia coli growing on under anaerobic conditions at slightly alkaline pH. Biochem Biophys Res Commun. 2003 Jun 27;306(2):361-5. H (2) production is largely dependent on dehydrogenase H and hydrogenases 3 and 4 constituting two lyases, and on the F (0) F (1)-ATPase. This activity was significantly (1.8-fold) stimulated by 100mM K (+) and inhibited by N,N (')-dicyclohexylcarbodiimide and sodium azide. |
5(0,0,0,5) | Details |
| 11742758 | Da Silva RS, de Paula Cognato G, Bogo MR, da Graca Fauth M, Fin CA, Thome JW, Bonan CD, Dutra Dias R: Unique Ca (2+)-activated ATPase in the nervous ganglia of Phyllocaulis soleiformis (Mollusca). Comp Biochem Physiol B Biochem Mol Biol. 2002 Jan;131(1):55-61. Ca (2+)-ATPase activity was insensitive to the classical ATPase inhibitors ouabain, N-ethylmaleimide, orthovanadate and sodium azide. |
86(1,1,1,6) | Details |
| 8083192 | RayChaudhuri D, Park JT: A point mutation converts Escherichia coli FtsZ septation GTPase to an ATPase. J Biol Chem. 1994 Sep 16;269(37):22941-4. This activity, unlike the wild-type GTPase, is specifically inhibited by sodium azide, a known antagonist of F-type ATPases and the bacterial SecA protein translocation ATPase (Oliver, D., Cabelli, R. |
86(1,1,1,6) | Details |
| 11691603 | Furriel RP, McNamara JC, Leone FA: as a tool to characterize gill Na (+), K (+)-ATPase activity in hyperregulating Crustacea. Comp Biochem Physiol A Mol Integr Physiol. 2001 Nov;130(4):665-76. Ouabain (K (i)=762.4+/-26.7 micromol l (-1)) and orthovanadate (K (i)=0.25+/-0.01 micromol l (-1)) completely inhibited the K (+)-phosphatase activity, while thapsigargin, oligomycin, sodium azide and bafilomycin were without effect. |
4(0,0,0,4) | Details |
| 15488293 | Grammatopoulos TN, Johnson V, Moore SA, Andres R, Weyhenmeyer JA: Angiotensin type 2 receptor neuroprotection against chemical hypoxia is dependent on the delayed rectifier K+ channel, Na+/Ca2+ exchanger and Na+/K+ ATPase in primary cortical cultures. Neurosci Res. 2004 Nov;50(3):299-306. Here, we show in mouse primary neuronal cultures that the AT (2) receptor neuroprotection results from the activation of the delayed rectifier K (+) channel as well as the involvement of the Na (+)/Ca (2+) exchanger (NCX) and Na (+)/K (+) ATPase (ATPase). Sodium azide (10mM) induced apoptosis in 40% of neurons. |
4(0,0,0,4) | Details |
| 11955083 | Miller A, Wang L, Kendall DA: SecB modulates the nucleotide-bound state of SecA and stimulates ATPase activity. Biochemistry. 2002 Apr 23;41(16):5325-32. The inhibition of SecA ATPase activity by sodium azide in the presence of IMVs and a functional signal peptide further indicates that SecB promotes the ATP-bound form of SecA. |
85(1,1,1,5) | Details |
| 19961421 | Sen SS, Bhuyan NR, Lakshman K, Roy AK, Chakraborty B, Bera T: Membrane bound pyrophosphatase and P-type triphosphatase of Leishmania donovani as possible chemotherapeutic targets: similarities and differences in inhibitor sensitivities. Biochemistry. 2009 Dec;74(12):1382-7. ATPase activity was stimulated by K+ and inhibited by DCCD, PCMBS, NEM, sodium azide, orthovanadate, and and was unaffected by PAO. |
84(1,1,1,4) | Details |
| 17215890 | Aurade R, Jayalakshmi SK, Sreeramulu K: Stimulatory effect of insecticides on partially purified P-glycoprotein ATPase from the resistant pest Helicoverpa armigera. Biochem Cell Biol. 2006 Dec;84(6):1045-50. Other inhibitors, such as EDTA, sodium azide, and resulted in only a 20% decrease in activity. |
4(0,0,0,4) | Details |
| 8687417 | Docampo R, Vanderheyden NM, Shaw MM, Durant PJ, Bartlett MS, Smith JW, McLaughlin GL: An H (+)-ATPase regulates cytoplasmic pH in Pneumocystis carinii trophozoites. Biochem J. 1996 Jun 1;316 ( Pt 2):681-4. In addition, steady-state pHi depended on the availability of cellular ATP, since it was decreased by the ATP synthase inhibitors oligomycin (1 microgram/ml) and sodium azide (1 mM), and by the uncoupler of oxidative phosphorylation carbonyl p-trifluorophenylhydrazone (1 microM), agents that were able to deplete significantly the intracellular ATP levels. |
3(0,0,0,3) | Details |
| 11007172 | Furriel RP, McNamara JC, Leone FA: Characterization of (Na+, K+)-ATPase in gill microsomes of the freshwater shrimp Macrobrachium olfersii. Comp Biochem Physiol B Biochem Mol Biol. 2000 Jul;126(3):303-15. The remaining 30% activity was inhibited by oligomycin, sodium azide and bafilomycin A. |
0(0,0,0,0) | Details |
| 16982198 | Hiruma H, Katakura T, Takenami T, Igawa S, Kanoh M, Fujimura T, Kawakami T: Vesicle disruption, plasma membrane bleb formation, and acute cell death caused by illumination with blue light in acridine orange-loaded malignant melanoma cells. J Photochem Photobiol B. 2007 Jan 3;86(1):1-8. Epub 2006 Sep 18. Both the vesicle disruption and the formation of plasma membrane blebs were inhibited by removal of from the cell environment and by singlet scavengers, sodium azide, and but not inhibited by the scavenger dimethyl thiourea. |
0(0,0,0,0) | Details |
| 11815454 | Komatsu M, Sato Y, Yamada S, Yamauchi K, Hashizume K, Aizawa T: Triggering of insulin release by a combination of cAMP signal and nutrients: an ATP-sensitive K+ channel-independent phenomenon. Diabetes. 2002 Feb;51 Suppl 1:S29-32. As anticipated, the action of and KIC was greatly (> 80%) suppressed by inhibition of mitochondrial metabolism by 2 mmol/l sodium azide (NaN (3)). |
0(0,0,0,0) | Details |
| 8553371 | Bergesse JR, Balegno HF: 2,4-Dichlorophenoxyacetic acid influx is mediated by an active transport system in Chinese hamster ovary cells. Toxicol Lett. 1995 Nov 15;81(2-3):167-73. The uptake of 2,4-D was carried out against a concentration gradient and was inhibited by sodium azide and dinitrophenol. |
0(0,0,0,0) | Details |
| 12901360 | McConnell EJ, Raess BU: Intracellular Ca2+ homeostatic regulation and -induced aortic endothelial dysfunction. Endothelium. 2002;9(1):45-53. The aldehydic lipid peroxidation product (HNE) is known to compromise erythrocyte passive Ca2+ permeability and to irreversibly inhibit the plasma membrane (Ca2+ + Mg2+)-ATPase and Ca2+-transport. This Ca2+ pool was diminished by thapsigargin, cyclopiazonic acid, oligomycin B, and sodium azide. |
1(0,0,0,1) | Details |
| 19293237 | Mahad DJ, Ziabreva I, Campbell G, Lax N, White K, Hanson PS, Lassmann H, Turnbull DM: Mitochondrial changes within axons in multiple sclerosis. . Brain. 2009 May;132(Pt 5):1161-74. Epub 2009 Mar 17. The lack of complex IV activity in a proportion of Na (+)/K (+) ATPase alpha-1 positive demyelinated axons supports axonal dysfunction as a contributor to neurological impairment and disease progression. |
1(0,0,0,1) | Details |
| 12450409 | Bandyopadhyay S, Valder CR, Huynh HG, Ren H, Allison WS: The beta G156C substitution in the F1-ATPase from the thermophilic Bacillus PS3 affects catalytic site cooperativity by destabilizing the closed conformation of the catalytic site. Biochemistry. 2002 Dec 3;41(48):14421-9. |
1(0,0,0,1) | Details |
| 9144325 | Menezes de Oliveira E, Oliveira Battastini AM, Meirelles MN, Menezes Moreira C, Dutra Dias R, Freitas Sarkis JJ: Characterization and localization of an ATP diphosphohydrolase activity (EC 3.6.1.5) in sarcolemmal membrane from rat heart. Mol Cell Biochem. 1997 May;170(1-2):115-23. It is Ca2+ dependent and is insensitive to ouabain, orthovanadate, N-ethylmaleimide (NEM), lanthanum, and oligomycin that are classical ATPase inhibitors. Sodium azide that is a mitochondrial inhibitor at low concentrations, did not affect the enzyme activity at 5.0 mM or below. |
1(0,0,0,1) | Details |
| 15701621 | Ma TY, Boivin MA, Ye D, Pedram A, Said HM: Mechanism of TNF-{alpha} modulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. Am J Physiol Gastrointest Liver Physiol. 2005 Mar;288(3):G422-30. Moreover, inhibitors of MLCK, Mg (2+)-myosin ATPase, and metabolic energy prevented the TNF-alpha increase in Caco-2 TJ permeability, suggesting that the increase in MLCK activity was required for the TNF-alpha-induced opening of the Caco-2 TJ barrier. |
1(0,0,0,1) | Details |
| 11374879 | Denda M, Ashida Y, Inoue K, Kumazawa N: Skin surface electric potential induced by ion-flux through epidermal cell layers. Biochem Biophys Res Commun. 2001 Jun 1;284(1):112-7. Disruption of mitochondrial function and inhibition of ATPase reduced the skin surface potential 50-70%. |
1(0,0,0,1) | Details |
| 7761265 | Ziganshin AU, Ziganshina LE, King BE, Burnstock G: Characteristics of ecto-ATPase of Xenopus oocytes and the inhibitory actions of suramin on ATP breakdown. Pflugers Arch. 1995 Jan;429(3):412-8. Ca2+/Mg (2+)-dependent ecto-ATPase was active over a limited range of 0.01-1.0 mM ATP, while Ca2+/Mg (2+)-independent ATPase activity was active over a range of 0.1-30 mM ATP. Ecto-ATPase activity was unaffected by ouabain (100 microM), sodium azide (100 microM), and oligomycin (5 micrograms/ml) (as inhibitors of endo-ATPases) and beta-glycerophosphate (10 mM) and p-nitrophenyl (10 mM) (as inhibitors of non-specific alkaline phosphatase). |
1(0,0,0,1) | Details |
| 8021205 | Chun SY, Randall LL: In vivo studies of the role of SecA during protein export in Escherichia coli. J Bacteriol. 1994 Jul;176(14):4197-203. SecA is found in Escherichia coli both tightly associated with the cytoplasmic membrane where it functions as a translocation ATPase during protein export and free in the cytosol (R. With the aim of determining the roles of the multiple binding sites for ATP on SecA, we compared the export defect in a culture of E. coli expressing a temperature-sensitive allele of secA with the defect in a culture treated with sodium azide. |
1(0,0,0,1) | Details |
| 11862992 | Coimbra ES, Goncalves-da-Costa SC, Corte-Real S, De Freitas FG, Durao AC, Souza CS, Silva-Santos MI, Vasconcelos EG: Characterization and cytochemical localization of an ATP diphosphohydrolase from Leishmania amazonensis promastigotes. Parasitology. 2002 Feb;124(Pt 2):137-43. Sodium azide (5-10 mM) caused inhibition of the ATP and ADP hydrolysis in a dose-dependent manner. was the best activating metal ion for both ATPase and ADPase activities. |
1(0,0,0,1) | Details |
| 14629005 | Alves-Ferreira M, Dutra PM, Lopes AH, Ferreira-Pereira A, Scofano HM, Meyer-Fernandes JR: -dependent ecto-ATP diphosphohydrolase activity in Herpetomonas muscarum muscarum. Curr Microbiol. 2003 Oct;47(4):265-71. The ecto-ATPase activity was insensitive to oligomycin and sodium azide, two inhibitors of mitochondrial Mg-ATPase, bafilomycin A1, a V-ATPase inhibitor, ouabain, a Na (+)+K+-ATPase inhibitor and to levamizole, an inhibitor of alkaline phosphatase. The ecto-ATPase activity was insensitive to oligomycin and sodium azide, two inhibitors of mitochondrial Mg-ATPase, bafilomycin A1, a V-ATPase inhibitor, ouabain, a Na (+)+K+-ATPase inhibitor and to levamizole, an inhibitor of alkaline phosphatase. |
1(0,0,0,1) | Details |
| 11584374 | Ujhazy P, Ortiz D, Misra S, Li S, Moseley J, Jones H, Arias IM: Familial intrahepatic cholestasis 1: studies of localization and function. . Hepatology. 2001 Oct;34(4 Pt 1):768-75. In canalicular membrane vesicles, translocase activity had an initial velocity of 3.3 nmol (PS) translocated per milligram of protein per minute and a K (m) (ATP) = 1.2 mmol/L; was inhibited by vanadate, N-ethylmaleimide, sodium azide, and and was unidirectional (i.e., from the outer to the inner canalicular plasma membrane leaflet). These studies indicate that FIC1 is a canalicular P-type ATPase that participates in maintaining the distribution of aminophospholipids between the inner and outer leaflets of the plasma membrane. |
1(0,0,0,1) | Details |
| 15050517 | Borges E, Cognato Gde P, Vuaden FC, Bogo MR, Fauth Mda G, Bonan CD, Dias RD: Nucleotidase activities in membrane preparations of nervous ganglia and digestive gland of the snail Helix aspersa. Comp Biochem Physiol B Biochem Mol Biol. 2004 Mar;137(3):297-307. ATPase and ADPase activities were dependent on Ca2+ and Mg2+ with pH optima approximately 7.2 and between 6.0 and 8.0 in digestive gland and nervous ganglia, respectively. Sodium azide, at 100 microM and 20 mM, inhibited Mg (2+)-ATP hydrolysis by 36% and 55% in digestive gland, respectively. |
1(0,0,0,1) | Details |
| 8856086 | Balasubramanian K, Gupta CM: Transbilayer phosphatidylethanolamine movements in the yeast plasma membrane. Eur J Biochem. 1996 Sep 15;240(3):798-806. The levels of external PtdEtn in the intact cells were reduced to about 50% by pretreatment of the cells with inhibitors of mitochondrial ATP synthesis, ATPase inhibitors or protein-sulfhydryl-group-modifying reagents, or by depletion of the cells of ATP by metabolic starvation. |
1(0,0,0,1) | Details |
| 14659655 | Hall IH, Schwab UE, Ward ES, Ives T: Disposition and intracellular levels of moxifloxacin in human THP-1 monocytes in unstimulated and stimulated conditions. Int J Antimicrob Agents. 2003 Dec;22(6):579-87. The -ATPase pump when blocked, also retarded moxifloxacin uptake at 2 and 4 h. |
1(0,0,0,1) | Details |
| 9729436 | Kunioka E, Matsuyama S, Tokuda H: Cloning and expression of the secA gene of a marine bacterium, Vibrio alginolyticus, and analysis of its function in Escherichia coli. Gene. 1998 Aug 31;216(2):303-9. The endogenous ATPase activity of the purified VaSecA was comparable with that of EcSecA. 1998 Elsevier Science B.V. |
1(0,0,0,1) | Details |