| 11683395 |
Parton RM, Fischer-Parton S, Watahiki MK, Trewavas AJ: Dynamics of the apical vesicle accumulation and the rate of growth are related in individual pollen tubes. J Cell Sci. 2001 Jul;114(Pt 14):2685-95.
To examine the organisation and dynamics of membrane movements at the growing pollen tube apex and address the question of their relationship to growth, we have used the membrane stain FM4-64 both as a structural marker and as a quantitative assay.
Dye uptake could be inhibited by sodium azide and followed a strict temporal sequence from the plasma membrane to a population of small (1-2 microm diameter) discrete internal structures, with subsequent appearance of dye in the apical region and ultimately in vacuolar membranes. |
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