Protein Information

Name NADP oxidoreductase
Synonyms G6PD; G6PD1; G6PDH; Glucose 6 phosphate 1 dehydrogenase; Glucose 6 phosphate dehydrogenase; NADP oxidoreductase; glucose 6 phosphate; Glucose 6 phosphate 1 dehydrogenases…

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
16046670 Frederiks WM, van Marle J, van Oven C, Comin-Anduix B, Cascante M: Improved localization of glucose-6-phosphate dehydrogenase activity in cells with 5-cyano-2,3-ditolyl-tetrazolium chloride as fluorescent redox dye reveals its cell cycle-dependent regulation. J Histochem Cytochem. 2006 Jan;54(1):47-52. Epub 2005 Jul 26.

Cells were incubated for the detection of G6PD activity using a medium containing 6% (w/v) polyvinyl alcohol, 5 mM CTC, magnesium chloride, sodium azide, the electron carrier methoxyphenazine methosulphate, NADP, and glucose-6-phosphate.
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8071087 Kugler P: Glucose-6-phosphate dehydrogenase is enriched in oligodendrocytes of the rat spinal cord. Histochemistry. 1994 Feb;101(2):143-53.

Methoxyphenazine methosulphate as an exogenous electron carrier and sodium azide as a blocker of the respiratory chain significantly increased the demonstrable G6PD activity.
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12900405 Emerson JP, Coulter ED, Phillips RS, Kurtz DM Jr: Kinetics of the superoxide reductase catalytic cycle. J Biol Chem. 2003 Oct 10;278(41):39662-8. Epub 2003 Aug 4.


A proximal electron donor, rubredoxin, was used to supply reducing equivalents from NADPH via ferredoxin: NADP+ oxidoreductase, and xanthine/xanthine oxidase was used to provide a calibrated flux of superoxide.
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