Protein Information

Name laminin (protein family or complex)
Synonyms Laminin; Laminins

Compound Information

Name sodium azide
CAS sodium azide

Reference List

PubMed Abstract RScore(About this table)
8548838 Schroen DJ, Cheung HT: Interaction of mouse thymocytes and a thymocyte-like cell line with the ECM glycoprotein entactin. Cell Immunol. 1996 Jan 10;167(1):141-9.

Preincubation of the S49.1 cells in medium containing the metabolic inhibitors sodium azide or 2-deoxy-D-glucose inhibited adhesion to entactin 47.2 and 79.5%, respectively.
A high percentage (approximately 90-100%) of S49.1 cells also bound to the lectin concanavalin A and to fibronectin, while laminin promoted only 19.3% adhesion.
3(0,0,0,3) Details
8406852 Ollert MW, Sohnchen R, Korting HC, Ollert U, Brautigam S, Brautigam W: Mechanisms of adherence of Candida albicans to cultured human epidermal keratinocytes. Infect Immun. 1993 Nov;61(11):4560-8.

CDPGYIGSR-NH2, which is a synthetic adhesive peptide derived from the laminin B chain, was much more efficient in its inhibitory activity than the RGD peptides and reduced C. albicans adherence to cultured human keratinocytes up to 76% (P < 0.001).
C. albicans adherence was blocked by the acid protease inhibitor pepstatin A and the metabolic inhibitor sodium azide.
2(0,0,0,2) Details
8751977 Herrmann G, Wlaschek M, Bolsen K, Prenzel K, Goerz G, Scharffetter-Kochanek K: Photosensitization of uroporphyrin augments the ultraviolet A-induced synthesis of matrix metalloproteinases in human dermal fibroblasts. J Invest Dermatol. 1996 Sep;107(3):398-403.

In this study, we therefore asked whether long-wave ultraviolet irradiation (340-450 nm) in conjunction with uroporphyrin I could modulate the synthesis of MMPs with substrate specificities for dermal (collagens I, III, V; proteoglycans) and basement membrane components (collagens IV, VII; fibronectin; laminin) and whether synthesis of the counteracting tissue inhibitor of metalloproteinases is also affected.
Using deuterium oxide as enhancer and sodium azide as quencher of singlet oxygen, we could increase or reduce MMP synthesis, suggesting that singlet oxygen is the major intermediate in the upregulation of MMPs after irradiation of uroporphyrin-pretreated fibroblasts.
1(0,0,0,1) Details
8769278 Ebert EC: Mechanisms of colon cancer binding to substratum and cells. Dig Dis Sci. 1996 Aug;41(8):1551-6.

Binding began within minutes and peaked by 1 hr, with 80-95% of HT-29 cells binding to the ECM proteins, collagen IV, laminin, fibronectin, and vitronectin and 40-75% binding to monolayers of fibroblasts, smooth muscle cells, and HT-29 cells.
Attachment of HT-29 cells to cell monolayers was inhibited by cytochalasin D and sodium azide, but not cycloheximide or neuraminidase.
1(0,0,0,1) Details
10362101 Runnels JM, Chen N, Ortel B, Kato D, Hasan T: BPD-MA-mediated photosensitization in vitro and in vivo: cellular adhesion and beta1 integrin expression in ovarian cancer cells. Br J Cancer. 1999 Jun;80(7):946-53.

Mild BPD-MA photosensitization (approximately 85% cell survival) of OVCAR 3 transiently decreased adhesion to collagen IV, fibronectin, laminin and vitronectin to a greater extent than could be attributed to cell death.
Photosensitized adhesion was reduced in the presence of sodium azide and enhanced in deuterium oxide, suggesting mediation by singlet oxygen.
1(0,0,0,1) Details