Protein Information

Name Cytochrome c oxidase (protein family or complex)
Synonyms COX; cytochrome c oxidase; cytochrome c oxidases

Compound Information

Name mercuric chloride
CAS

Reference List

PubMed Abstract RScore(About this table)
18603796 Sugio T, Fujii M, Ninomiya Y, Kanao T, Negishi A, Takeuchi F: Reduction of Hg2+ with reduced mammalian cytochrome c by cytochrome c oxidase purified from a mercury-resistant acidithiobacillus ferrooxidans strain, MON-1. Biosci Biotechnol Biochem. 2008 Jul;72(7):1756-63. Epub 2008 Jul 7.

The cytochrome c oxidase activities of strains AP19-3 and ATCC 23270 were completely inhibited by 1 muM and 5 muM of mercuric chloride respectively.
65(0,2,2,5) Details
1655772 Pan LP, He QZ, Chan SI: The nature of zinc in cytochrome c oxidase. . J Biol Chem. 1991 Oct 5;266(28):19109-12.

Although the treatment of cytochrome c oxidase with mercuric chloride inhibits the steady-state activity of the enzyme, subsequent removal of the Hg2+ bound to cysteine residues by 1,5-I-AEDANS significantly reverses the inhibition.
38(0,1,2,3) Details
16297871 Whiteman M, Chua YL, Zhang D, Duan W, Liou YC, Armstrong JS: Nitric oxide protects against mitochondrial permeabilization induced by glutathione depletion: role of S-nitrosylation?. Biochem Biophys Res Commun. 2006 Jan 6;339(1):255-62. Epub 2005 Nov 10.

Nitric oxide (NO) is known to mediate a multitude of biological effects including inhibition of respiration at cytochrome c oxidase (COX), formation of peroxynitrite (ONOO-) by reaction with mitochondrial superoxide (O2*-), and S-nitrosylation of proteins.
However, NO increased the level of mitochondrial protein S-nitrosylation (SNO) determined by the Biotin Switch assay and by the release of NO from mitochondrial fractions treated with mercuric chloride (which cleaves SNO bonds to release NO).
1(0,0,0,1) Details