Protein Information

Name beta adrenergic receptors (protein family or complex)
Synonyms Beta adrenoceptor; Beta adrenoceptor; Beta adrenergic receptor; Beta adrenergic receptors; Beta adrenoceptor; Beta adrenoceptors; Beta adrenoceptors

Compound Information

Name diphenylamine
CAS

Reference List

PubMed Abstract RScore(About this table)
9218212 Chan HC, Fong SK, So SC, Chung YW, Wong PY: Stimulation of anion secretion by beta-adrenoceptors in the mouse endometrial epithelium. J Physiol. 1997 Jun 15;501 ( Pt 3):517-25.

The adrenaline-induced ISC could be inhibited by pretreatment with diphenylamine 2,2'-dicarboxylic acid (DPC) or replacement of external Cl- and HCO3-, but not by amiloride or replacement of Na+ in apical solution. 3.
3(0,0,0,3) Details
10924327 Mizuno Y, Ito Y, Aoyama M, Kume H, Nakayama S, Yamaki K: Imipramine inhibits Cl (-) secretion by desensitization of beta-adrenergic receptors in calu-3 human airway cells. Biochem Biophys Res Commun. 2000 Aug 11;274(3):620-5.

ISP applied in the basolateral solution generated a sustained short-circuit current that was abolished by diphenylamine-2-carboxylate, a Cl (-) channel blocker.
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1351109 Tamaoki J, Kondo M, Chiyotani A, Yamauchi F, Takeuchi S, Konno K: [Airway epithelial beta 3-adrenergic receptor--effect on bioelectric properties and its mechanism of action]. Nihon Kyobu Shikkan Gakkai Zasshi. 1992 Feb;30(2):310-6.

To characterize the "atypical" beta-adrenergic receptor (beta 3-adrenergic receptor) and its action on ion transport across airway mucosa, we measured the bioelectric properties of canine cultured tracheal epithelium under short-circuited conditions in vitro.
This effect on Isc was accompanied by the accumulation of intracellular cyclic AMP, and it was abolished by diphenylamine-2-carboxylate, bumetanide, and Cl-free medium, but not by amiloride.
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7539220 Dinudom A, Komwatana P, Young JA, Cook DI: A forskolin-activated Cl- current in mouse mandibular duct cells. Am J Physiol. 1995 May;268(5 Pt 1):G806-12.

The current carried by this conductance is attenuated 65% by 1 mmol/l diphenylamine-2-carboxylate but is not affected by 0.1 mmol/l4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid or 0.1 mmol/l glibenclamide.
The current can be activated by norepinephrine (1 mumol/l), evidently acting via beta-adrenergic receptors, since the effect of norepinephrine is inhibited by propranolol (1 mumol/l).
1(0,0,0,1) Details
12466941 Cuffe JE, Howard DP, Bertog M, Korbmacher C: Basolateral adrenoceptor activation mediates noradrenaline-induced Cl- secretion in M-1 mouse cortical collecting duct cells. Pflugers Arch. 2002 Dec;445(3):381-9. Epub 2002 Nov 1.

We conclude that in M-1 cells NA stimulates Cl (-) secretion probably involving both alpha (1)- and beta-adrenoceptors located basolaterally.
In contrast, the response was largely reduced in the presence of apical diphenylamine-2-carboxylic acid (1 mM) and in the absence of extracellular Cl (-).
1(0,0,0,1) Details
11138724 Liu W, Sato Y, Hosoda Y, Hirasawa K, Hanai H: Effects of higenamine on regulation of ion transport in guinea pig distal colon. Jpn J Pharmacol. 2000 Nov;84(3):244-51.


The initial phase with an increase in Isc was partially inhibited by serosal bumetanide and abolished by mucosal diphenylamine-2-carboxylate, a chloride channel blocker, indicating transient induction of Cl- secretion.
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