| Name | adenylate cyclase |
|---|---|
| Synonyms | AH related protein; HCA2; ATP pyrophosphate lyase; Adenylate cyclase; Adenylyl cyclase soluble; HCA 2; RP1 313L4.2; SAC… |
| Name | diphenylamine |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 9218212 | Chan HC, Fong SK, So SC, Chung YW, Wong PY: Stimulation of anion secretion by beta-adrenoceptors in the mouse endometrial epithelium. J Physiol. 1997 Jun 15;501 ( Pt 3):517-25. The effect of on the ISC was mimicked by an adenylate cyclase activator, forskolin, but suppressed by the adenylate cyclase inhibitor MDL 12,330A, indicating the involvement of cAMP. 7. The -induced ISC could be inhibited by pretreatment with diphenylamine 2,2'-dicarboxylic acid (DPC) or replacement of external Cl- and HCO3-, but not by amiloride or replacement of Na+ in apical solution. 3. |
1(0,0,0,1) | Details |
| 10381142 | Panesar NS: Role of and inhibitory action of inorganic on gonadotropin-stimulated steroidogenesis in mouse Leydig tumor cells. Metabolism. 1999 Jun;48(6):693-700. The involvement of adenylate cyclase-cyclic (AC-cAMP) in gonadotropin-stimulated testicular steroidogenesis is well known. A chloride-channel inhibitor, diphenylamine-2-carboxylate (DPC), at concentrations up to 0.6 mmol/L stimulated basal steroid secretion and hCG 10 IU/L stimulated cAMP production, but higher concentrations had an inhibitory effect. |
1(0,0,0,1) | Details |
| 10611078 | Leung GP, Wong PY: Activation of cystic fibrosis transmembrane conductance regulator in rat epididymal epithelium by Biol Reprod. 2000 Jan;62(1):143-9. The response could be blocked by the nonspecific Cl (-) channel blocker, diphenylamine-2-carboxylate (DPC), but not by the Ca (2+)-activated Cl (-) channel blocker, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). Moreover, pretreatment of the tissues with MDL-12330A, an adenylate cyclase inhibitor, markedly attenuated the Isc response to but the response was restored upon the addition of exogenous cAMP. |
1(0,0,0,1) | Details |
| 16760378 | Du JY, Ruan YC, Zuo WL, Yang ZH, Chen MH, Wu ZL, Xiang H, Zhou WL: Cellular mechanisms of carbachol-stimulated Cl- secretion in rat epididymal epithelium. Biol Reprod. 2006 Sep;75(3):407-13. Epub 2006 Jun 7. Ca2+ activated Cl- channel blocker disulfonic acid stilbene (DIDS, 300 microM) only inhibited part of the CCH-induced Isc response, while nonselective Cl- channel blocker diphenylamine-dicarboxylic acid (DPC, 1 mM) reduced all, indicating the involvement of different conductance pathways. Both peaks of the CCH-induced Isc response could be significantly inhibited by pretreatment with an adenylate cyclase inhibitor, MDL12330A (50 microM). |
1(0,0,0,1) | Details |
| 16015685 | He Q, Zhu JX, Xing Y, Tsang LL, Yang N, Rowlands DK, Chung YW, Chan HC: Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents. World J Gastroenterol. 2005 Jul 21;11(27):4173-9. RESULTS: TMP stimulated a concentration-dependent rise in I (SC), which was dependent on both Cl (-) and HCO (3)(-), and inhibited by apical application of diphenylamine-2,2'-dicarboxylic acid (DPC) and glibenclamide, but resistant to 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid disodium salt hydrate (DIDS). RIA results showed that TMP (1 mmol/L) elicited a significant increase in cellular cAMP production, which was similar to that elicited by the adenylate cyclase activator, forskolin (10 micromol/L). |
1(0,0,0,1) | Details |
| 16038730 | Assef YA, Cavarra SM, Damiano AE, Ibarra C, Kotsias BA: Ionic currents in multidrug resistant K562 human leukemic cells. Leuk Res. 2005 Sep;29(9):1039-47. Epub 2005 Apr 19. Forskolin (40 microM n activator of adenylate cyclase, added to the extracellular side increased the current in both cell lines. A fraction of the activated whole cell currents was inhibited by 500 microM 4,4-diisothiocyanatostilbene-2,2-disulfonic acid (DIDS) and subsequent addition of 500 microM diphenylamine-2-carboxylate (DPC plus DIDS) further inhibited the remaining currents. |
1(0,0,0,1) | Details |
| 9224546 | Saidane D, Lahlou B, Tritar B: Regional variations in electrical and ion transport properties along the isolated intestine of the frog Rana esculenta. Arch Physiol Biochem. 1997 Feb;105(1):45-52. Na+/K+/2Cl- cotransport (current decreased by about 50% by bumetanide in anterior and posterior regions only), Cl- permeability or channels inhibited by diphenylamine-2-carboxylate, DPC (similar decreases as by bumetanide). Finally, current increase by IBMX and designate the colon as a target for adenylate cyclase stimulating hormones. |
1(0,0,0,1) | Details |
| 8743460 | Sullivan LP, Grantham JJ: Mechanisms of fluid secretion by polycystic epithelia. Kidney Int. 1996 Jun;49(6):1586-91. These preparations absorb fluid in the control state but secrete fluid in response to native cyst fluid, to adenylate cyclase agonists and to permeant analogues of cAMP. |
1(0,0,0,1) | Details |
| 8447435 | Siga E, Mandon B, Roinel N, de Rouffignac C: Effects of calcitonin on function of intercalated cells of rat cortical collecting duct. Am J Physiol. 1993 Feb;264(2 Pt 2):F221-7. In the rat cortical collecting duct (CCD), the presence of highly specific receptors to calcitonin (CT) coupled to a sensitive adenylate cyclase system suggests that this segment is a target site for CT. The CT-induced lumen-positive PDte was reduced by 2.3 +/- 0.8 mV after the basolateral addition of the Cl- channel inhibitor diphenylamine-2-carboxylate. 4-Acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid and acetazolamide, which inhibit Cl-/HCO3- exchangers and carbonic anhydrase activities, respectively, also inhibited the CT-induced PDte by 4.6 +/- 0.5 and 5.0 +/- 0.9 mV. |
1(0,0,0,1) | Details |
| 9379115 | Zhou WL, Leung PS, Wong TP, Dun NJ, Wong PY, Chan HC: Local regulation of epididymal anion secretion by pituitary adenylate cyclase-activating polypeptide. J Endocrinol. 1997 Sep;154(3):389-95. The Isc response to PACAP38 was abolished in Cl-(-) free solution and completely inhibited by the Cl- channel blocker, diphenylamine-dicarboxylic acid. The Isc response to PACAP38 was also suppressed by pretreatment of the cells with the adenylate cyclase inhibitor, MDL12330A. |
1(0,0,0,1) | Details |
| 18784353 | Du JY, Zuo WL, Ruan YC, Yang ZH, Chen MH, Chen SL, Li S, Wu ZL, Xiang H, Zhou WL: Stimulating effects of on transport across the rat caudal epididymal epithelium in culture. Biol Reprod. 2009 Jan;80(1):13-23. Epub 2008 Sep 10. The response was inhibited by the apical Cl- channel blocker, diphenylamine-dicarboxylic acid, and the Ca2+-activated Cl- channel blocker, disulfonic acid stilbene, indicating that Cl- may pass through two types of Cl- channels on the apical side. Pretreating the monolayers with an adenylate cyclase inhibitor, MDL12330A, inhibited all of the second Isc response and part of the initial spike. |
1(0,0,0,1) | Details |
| 2411931 | Cuthbert AW, Hickman ME: Indirect effects of on secretion in mammalian colon. J Membr Biol. 1985;86(2):157-66. Using inhibitors of NaCl cotransport (piretanide), carbonic anhydrase (acetazolamide), and channels (diphenylamine-2-carboxylate), it was concluded that the SCC response to ATP was due to secretion with, perhaps, a minor contribution from |
0(0,0,0,0) | Details |
| 12181292 | Cowley EA, Linsdell P: Oxidant stress stimulates anion secretion from the human airway epithelial cell line Calu-3: implications for cystic fibrosis lung disease. J Physiol. 2002 Aug 15;543(Pt 1):201-9. This increase was almost entirely abolished by the addition of diphenylamine-2-carboxylate (DPC), implicating the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel in the response. |
0(0,0,0,0) | Details |
| 9546734 | Fong SK, Chan HC: Regulation of anion secretion by in the mouse endometrial epithelium. Biol Reprod. 1998 Apr;58(4):1020-5. Pretreatment with diphenylamine 2,2'-dicarboxylic acid, a Cl- channel blocker, significantly reduced both -induced ISC responses, while pretreatment with amiloride, a Na+ channel blocker, did not exert any effect. Forskolin, an adenylate cyclase activator, and 3-isobutyl-dihydro--1-methyl- a cAMP phosphodiesterase inhibitor, mimicked the ISC response to while MDL12330A, an adenylate cyclase inhibitor, completely abolished the PGE2-induced ISC. |
1(0,0,0,1) | Details |
| 14715281 | Mahlangu DA, Dix JA: Halide fluxes in epithelial cells measured with an automated cell plate reader. Anal Biochem. 2004 Feb 1;325(1):28-34. Isoproterenol (a modulator of cAMP-activated channels) increased the exchange rate by a factor of 1.4+/-0.1; A23187 (a modulator of -activated channels) increased the rate by 3.4+/-0.4; bradykinin (also a modulator of -activated channels) increased the rate by 2.0+/-0.4; forskolin (a direct stimulator of adenylate cyclase) increased the rate by 2.7+/-0.3. Diphenylamine-2-carboxylate (a chloride channel blocker) decreased the rate by 0.12+/-0.03. |
1(0,0,0,1) | Details |
| 1708205 | Heisler S: Chloride channel blockers inhibit ACTH secretion from mouse pituitary tumor cells. Am J Physiol. 1991 Apr;260(4 Pt 1):E505-12. When cells were simultaneously exposed to diphenylamine-2-carboxylate (DPC) or related substances (Hoechst compounds 131, 143, and 144) and the adenylate cyclase activator forskolin, ACTH secretion was inhibited by 76-95% [half-maximal inhibitory concentration (IC50) 450, 15, 84, and 32 microM, respectively]. |
32(0,1,1,2) | Details |
| 8807590 | Davidow CJ, Maser RL, Rome LA, Calvet JP, Grantham JJ: The cystic fibrosis transmembrane conductance regulator mediates transepithelial fluid secretion by human autosomal dominant polycystic kidney disease epithelium in vitro. Kidney Int. 1996 Jul;50(1):208-18. We now report that forskolin, which stimulates adenylate cyclase, increased the efflux rate constant for 36Cl in monolayers of ADPKD cells in vitro from 0.23 +/- 0.02 min-1 to 0.44 +/- 0.05 min-1 (N = 4) and that diphenylamine 2-carboxylate (DPC), which blocks channels, eliminated the forskolin-stimulated efflux from these cells. |
31(0,1,1,1) | Details |
| 9916004 | Chan LN, Chung YW, Leung PS, Liu CQ, Chan HC: Activation of an 3',5'-cyclic monophosphate-dependent Cl- conductance in response to neurohormonal stimuli in mouse endometrial epithelial cells: the role of cystic fibrosis transmembrane conductance regulator. Biol Reprod. 1999 Feb;60(2):374-80. The agonist-activated current could be mimicked by an adenylate cyclase activator, forskolin (10 microM), and suppressed by an adenylate cyclase inhibitor, MDL12330A, suggesting the involvement of cAMP. The agonist-induced current was inhibited by the Cl- channel blocker diphenylamine 2,2'-dicarboxylic acid (DPC), but not by the Cl- channel blocker 4,4'-diisothiocyanatostibene-2, 2'-disulfonic acid (DIDS). |
1(0,0,0,1) | Details |
| 9627094 | Chan HC, Lui KM, Wong WS, Poon AM: Effect of on secretion by human colonic T84 cells. Life Sci. 1998;62(23):2151-8. The increase in I (SC) was also blocked by apical addition of two Cl- channel blockers, 4,4'-diisothiocyanatostibene-2,2'-disulfonic acid (DIDS) and diphenylamine-2-carboxylic acid (DPC), indicating that stimulated Cl- secretion. However, inhibitors for Gi-protein, adenylate cyclase or phospholipase C were found to be ineffective in inhibiting the -induced I (SC). |
2(0,0,0,2) | Details |
| 2821159 | Reuss L: Cyclic AMP inhibits Cl-/HCO3- exchange at the apical membrane of Necturus gallbladder epithelium. J Gen Physiol. 1987 Aug;90(2):173-96. Intracellular cAMP levels were raised by addition of either the phosphodiesterase inhibitor (3 X 10 (-3) M) or the adenylate cyclase activator forskolin (10 (-5) M) to the serosal bathing solution. Estimates of the conductive flux, obtained by either reducing simultaneously mucosal [Cl-] and [HCO3-] or lowering [Cl-] alone in the presence of a blocker of anion exchange (diphenylamine-2-carboxylate), indicate that elevation of intracellular cAMP inhibited the anion exchanger by approximately 50%. |
1(0,0,0,1) | Details |