Protein Information

Name interferon gamma
Synonyms IFG; IFI; IFN gamma; IFNG; IFNgamma; Immune interferon; Interferon gamma; Interferon gamma precursor…

Compound Information

Name diphenylamine
CAS

Reference List

PubMed Abstract RScore(About this table)
10998443 Trachtman H, Futterweit S, Mermelstein A, Metzger S, Pettei M Jr: Anti-apoptotic effect of L-arginine in cultured rat mesangial cells. Int J Mol Med. 2000 Oct;6(4):485-9.

Cultured rat mesangial cells were exposed for 24-48 h to one of the following four experimental conditions known to induce apoptosis: i) a cytokine combination consisting of interferon-gamma (50 U/ml), interleukin-1 (5 ng/ml), and LPS (10 microg/ml); ii) 0.75 mM hydrogen peroxide; iii) etoposide, 150 microg/ml; and iv) cis-platinum, 100 microg/ml.
This effect was demonstrable by gel electrophoresis, diphenylamine assay of DNA fragmentation, and an ELISA to detect DNA oligonucleosomes.
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11108134 von Knethen A, Brune B: Attenuation of macrophage apoptosis by the cAMP-signaling system. Mol Cell Biochem. 2000 Sep;212(1-2):35-43.


Selected apoptotic parameters comprised DNA fragmentation (diphenylamine assay), annexin V staining of phosphatidylserine, caspase activity (quantitated by the cleavage of a fluorogenic caspase-3-like substrate Ac-DEVD-AMC), and mitochondrial membrane depolarisation (delta psi).
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10602776 von Knethen A, Brockhaus F, Kleiter I, Brune B: NO-Evoked macrophage apoptosis is attenuated by cAMP-induced gene expression. Mol Med. 1999 Oct;5(10):672-84.


Parameters comprised DNA fragmentation (diphenylamine assay), annexin V staining of phosphatidylserine, caspase activity (quantitated by the cleavage of a fluorogenic caspase-3-like substrate Ac-DEVD-AMC), and mitochondrial membrane depolarization (DeltaPsi), analyzed using DiOC (6)(3).
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20356634 Ni SJ, Qiu YH, Lu JH, Cao BB, Peng YP: Effect of cerebellar fastigial nuclear lesions on differentiation and function of thymocytes. J Neuroimmunol. 2010 Mar 29.

On days 12 and 32 following the KA injection, percentages of thymocyte subpopulations including CD4 (-) CD8 (-), CD4 (+) CD8 (+), CD4 (+) CD8 (-) and CD4 (-) CD8 (+) cells, apoptotic DNA fragmentation of thymocytes, and levels of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in the serum were measured by flow cytometry, diphenylamine assay and enzyme-linked immunosorbent assay (ELISA), respectively.
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20121592 Baker OJ, Schulz DJ, Camden JM, Liao Z, Peterson TS, Seye CI, Petris MJ, Weisman GA: Rat Parotid Gland Cell Differentiation in Three-Dimensional Culture. Tissue Eng Part C Methods. 2010 Mar 8.

These 3D Par-C10 acinar-like spheres display characteristics similar to differentiated acini in salivary glands, including cell polarization, tight junction (TJ) formation required to maintain transepithelial potential difference, basolateral expression of aquaporin-3 and Na (+)/K (+)/2Cl (-) cotransporter-1, and responsiveness to the muscarinic receptor agonist carbachol that is decreased by the anion channel blocker diphenylamine-2-carboxylic acid or chloride replacement with gluconate.
Further, the proinflammatory cytokines tumor necrosis factor-alpha and interferon-gamma induced alterations in TJ integrity in the acinar-like spheres without affecting individual cell viability, suggesting that cytokines may affect salivary gland function by altering TJ integrity.
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