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Bruno JG, Carrillo MP, Phillips T, King B: Development of DNA aptamers for cytochemical detection of acetylcholine. . In Vitro Cell Dev Biol Anim. 2008 Mar-Apr;44(3-4):63-72. Epub 2008 Feb 29.
Fixation with cold 2% paraformaldehyde was compared to cold alkaline allyl alcohol plus glutaraldehyde for immobilization of acetylcholine in situ and appeared to enable detection of greater numbers of cholinergic cells, although differences in levels of differentiation may have been a factor as well. |
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