Protein Information

Name alpha tubulin
Synonyms Alpha tubulin ubiquitous; Tubulin K alpha 1; Tubulin alpha ubiquitous chain; K alpha 1; K ALPHA 1 protein; alpha tubulin; K alpha 1s; K ALPHA 1 proteins…

Compound Information

Name trifluralin
CAS

Reference List

PubMed Abstract RScore(About this table)
19133502 Sheval EV, Kazhura YI, Poleshuk NA, Lazareva EM, Smirnova EA, Maximova NP, Polyakov VY: Trifluralin-induced disorganization of microtubular cytoskeleton alters the development of roots in Hordeum vulgare L. Acta Biol Hung. 2008 Dec;59(4):465-78.

Using antibodies to alpha-tubulin, we investigated the distribution of microtubules in root meristem cells.
1(0,0,0,1) Details
16913711 Mitra A, Sept D: Binding and interaction of dinitroanilines with apicomplexan and kinetoplastid alpha-tubulin. J Med Chem. 2006 Aug 24;49(17):5226-31.

In this work, through a series of computational studies, a common binding site of oryzalin, trifluralin, and GB-II-5 on apicomplexan and kinetoplastid alpha-tubulin is proposed.
33(0,1,1,3) Details
18180180 Yemets A, Radchuk V, Bayer O, Bayer G, Pakhomov A, Vance Baird W, Blume YB: Development of transformation vectors based upon a modified plant alpha-tubulin gene as the selectable marker. Cell Biol Int. 2008 May;32(5):566-70. Epub 2007 Nov 21.

Trifluralin, the main representative of dinitroaniline herbicides, was used as a selective agent in experiments to select transgenic cells, tissues and plantlets.
5(0,0,0,5) Details
12492843 Wiesler B, Wang QY, Nick P: The stability of cortical microtubules depends on their orientation. Plant J. 2002 Dec;32(6):1023-32.


We used tyrosinylated alpha-tubulin as a marker to assess auxin-dependent changes in microtubule turnover.
4(0,0,0,4) Details
19253757 Emets AI, Radchuk VV, Pakhomov AV, Blium IaB: [Biolictic transformation of soybean by new selective marker gene resistant to dinitroanilines]. Tsitol Genet. 2008 Nov-Dec;42(6):61-8.

Biolistic transformation of soybean by construction carrying a mutant alpha-tubulin gene as selective marker gene conferring resistance to dinitroaniline herbicides, and by additional construction pAHTUBI carrying a full-length barley beta-tubulin gene for correct co-expression of exogeneous tubulin in cells of transgenic soybean lines has been realized.
It was established that 10 microM trifluralin is the most optimal selective concentration to pick up transformed soybean lines.
2(0,0,0,2) Details
19050925 Giles NL, Armson A, Reid SA: Characterization of trifluralin binding with recombinant tubulin from Trypanosoma brucei. Parasitol Res. 2009 Mar;104(4):893-903. Epub 2008 Dec 3.

Secondly, they have a stronger affinity for trypanosomal alpha-tubulin compared with trypanosomal beta-tubulin.
2(0,0,0,2) Details
19947549 Emets AI, Baer OA, Radchuk VV, Blium IaB: [Agrobacterium-mediated transformation of flax with a mutant tubulin gene responsible for resistance to dinitroaniline herbicides]. Genetika. 2009 Oct;45(10):1377-85.

Agrobacterium tumefaciens was used to transform fiber flax with the pBITUBA8 plasmid carrying the mutant alpha-tubulin gene imparting resistance to dinitroaniline herbicides and the nptII selective marker gene imparting resistance to canamycin.
The transformants were selected in parallel on media containing canamycin and trifluralin (a dinitroaniline herbicide).
2(0,0,0,2) Details
17649622 Emets AI, Blium IaB: [Plant mutant tubulin genes as marker selective genes for genetic engineering]. Tsitol Genet. 2007 May-Jun;41(3):29-43.

The reports dealing with study the transferring and the expression of mutant tubulin genes conferring resistance to amiprophosmethyl (phosphorothioamidate herbicide) and to trifluralin (dinitroaniline herbicide) from corresponding N. plumbaginifolia mutants into related and remote plant species by somatic hybridisation methods have been analyzed.
The results of experiments on monocotyledonous and dicotyledonous. plant transformation by mutant alpha-tubulin gene conferring resistance to dinitroanilines are described to test the possibility of its using as a marker gene with obtaining, at the same time, a dinitroaniline-resistant plants.
1(0,0,0,1) Details
1673126 Schibler MJ, Huang B: The colR4 and colR15 beta-tubulin mutations in Chlamydomonas reinhardtii confer altered sensitivities to microtubule inhibitors and herbicides by enhancing microtubule stability. J Cell Biol. 1991 May;113(3):605-14.

The colR4 and colR15 mutants have been found to be cross resistant to vinblastine and several classes of antimitotic herbicides, including the dinitroanilines (oryzalin, trifluralin, profluralin, and ethafluralin); the phosphoric amide amiprophos methyl; and the dimethyl propynl benzamide pronamide.
Although wild-type like in their growth characteristics, the colR4 and colR15 mutants were found to have an altered pattern of microtubules containing acetylated alpha-tubulin, a posttranslational modification that has been associated with stable subsets of microtubules found in a variety of cells.
1(0,0,0,1) Details
9607761 Anthony RG, Waldin TR, Ray JA, Bright SW, Hussey PJ: Herbicide resistance caused by spontaneous mutation of the cytoskeletal protein tubulin. Nature. 1998 May 21;393(6682):260-3.

The dinitroaniline herbicides (such as trifluralin and oryzalin) have been developed for the selective control of weeds in arable crops.
Here we show that the major alpha-tubulin gene of the resistant biotype has three base changes within the coding sequence.
1(0,0,0,1) Details
11287173 Ovidi E, Gambellini G, Taddei AR, Cai G, Del Casino C, Ceci M, Rondini S, Tiezzi A: Herbicides and the microtubular apparatus of Nicotiana tabacum pollen tube: immunofluorescence and immunogold labelling studies. Toxicol In Vitro. 2001 Apr;15(2):143-51.


Using an antibody to the alpha-tubulin subunit in immunofluorescence and immunoelectron microscopy techniques, we investigated the distribution of microtubules in Nicotiana tabacum pollen tubes grown under in vitro conditions in the presence of five different herbicides selected among those used frequently in central Italy.
1(0,0,0,1) Details