| 330401 |
Takeda Y, Ogiso Y, Miwatani T: Effect of zinc ion on the hemolytic activity of thermostable direct hemolysin from Vibrio parahaemolyticus, streptolysin O, and Triton X-100. Infect Immun. 1977 Aug;17(2):239-43.
We found that in certain buffers, such as tris (hydroxymethyl)-aminomethane-hydrochloride, boric acid-borax, and N-hydroxyethyl piperazine-N'-2-ethanesulfonic acid-sodium hydroxide, hemoglobins released from erythrocytes were easily precipitated by addition of Zn2+, thus resulting in a false inhibition of hemolysin by Zn2+ when hemolysis was assayed by measuring absorbance at 540 nm of released hemoglobins. |
31(0,1,1,1) |
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| 19694483 |
Frasconi M, Rea S, Matricardi P, Favero G, Mazzei F: Scleroglucan-borax hydrogel: a flexible tool for redox protein immobilization. Langmuir. 2009 Sep 15;25(18):11097-104.
Heme proteins, such as hemoglobin (Hb), myoglobin (Mb), and horseradish peroxidase (HRP), were chosen as model proteins to investigate the immobilized system. |
1(0,0,0,1) |
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