Protein Information

Name epidermal growth factor
Synonyms EGF; Epidermal growth factor; Pro epidermal growth factor; Pro epidermal growth factor precursor; URG; Urogastrone; epidermal growth factor (beta urogastrone); Epidermal growth factors…

Compound Information

Name ferrous sulfate
CAS sulfuric acid iron(2+) salt (1:1)

Reference List

PubMed Abstract RScore(About this table)
2539287 Kumamoto S, Ono J, Tanaka Y, Takaki R: Serum-free culture of insulin-secreting clonal cells from a hamster insulinoma. Diabetes Res Clin Pract. 1989 Jan 3;6(1):1-7.

In addition to testing different varieties of basal media, we also experimented with different concentrations of known stimulants of cell proliferation, including transferrin, ferrous sulfate, insulin, epidermal growth factor, triiodothyronine, hydrocortisone, monoethanolamine, prolactin, proteose peptone, and selenium.
6(0,0,1,1) Details
9538899 Peng H, Wen TC, Tanaka J, Maeda N, Matsuda S, Desaki J, Sudo S, Zhang B, Sakanaka M: Epidermal growth factor protects neuronal cells in vivo and in vitro against transient forebrain ischemia- and free radical-induced injuries. J Cereb Blood Flow Metab. 1998 Apr;18(4):349-60.

2(0,0,0,2) Details
7040427 Tsao MC, Walthall BJ, Ham RG: Clonal growth of normal human epidermal keratinocytes in a defined medium. J Cell Physiol. 1982 Feb;110(2):219-29.

The defined medium consists of a basal medium, MCDB 152, supplemented with 5 ng/ml epidermal growth factor (EGF), 10 micrograms/ml transferrin, 5 micrograms/ml insulin, 1.4 X 10 (-6) M hydrocortisone, 1.0 X 10 (-5) Methanolamine, 1.0 X 10 (-5) M phosphoethanolamine, and 2.0 X 10 (-9) M progesterone.
Most of the requirement for transferrin, which is the least defined component of the defined medium, can be replaced by adding freshly dissolved and sterilized ferrous sulfate to the final medium after it has been filter sterilized.
1(0,0,0,1) Details