| 7607251 |
Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K: High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176. Eur J Biochem. 1995 Jun 1;230(2):773-8.
From carbamoylation with potassium cyanate and site-directed point mutagenesis of the cephalosporin C acylase, we have deduced that Tyr270 exists at a position where it can interact with a residue (possibly Ser239) corresponding to inactivation by carbamoylation. |
36(0,1,1,6) |
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