Protein Information

Name beta glucuronidase
Synonyms Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors

Compound Information

Name sulfuric acid
CAS sulfuric acid

Reference List

PubMed Abstract RScore(About this table)
18054529 Hauser B, Deschner T, Boesch C: Development of a liquid chromatography-tandem mass spectrometry method for the determination of 23 endogenous steroids in small quantities of primate urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Feb 1;862(1-2):100-12. Epub 2007 Nov 19.

The sample preparation consisted of an enzymatic hydrolysis of steroid glucuronides using beta-glucuronidase from E. coli followed by a solvolytic cleavage of steroid sulfates employing sulfuric acid/ethyl acetate.
81(1,1,1,1) Details
18574590 Baranowski S, Serr A, Thierauf A, Weinmann W, Grosse Perdekamp M, Wurst FM, Halter CC: In vitro study of bacterial degradation of ethyl glucuronide and ethyl sulphate. Int J Legal Med. 2008 Sep;122(5):389-93. Epub 2008 Jun 24.


Bacteria (Escherichia coli, Klebsiella pneumoniae, Clostridium sordellii) were isolated from autopsy material (liver, heart blood, urine, ascites, pericardial fluid, pleural fluid) tested for beta-glucuronidase activity, and three bacterial strains were added to nutrient-deficient medium containing EtG and/or EtS and incubated at 36 +/- 1 degrees C.
2(0,0,0,2) Details
16814335 Tseng YL, Sun CY, Kuo FH: Detection and quantification of glucuro- and sulfoconjugated metabolites in human urine following oral administration of xenobiotic 19-norsteroids. Steroids. 2006 Sep;71(9):817-27. Epub 2006 Jun 30.

In the present study, we investigated metabolites conjugated to a glucuronic acid and to a sulfuric acid in urine following administration of four xenobiotic 19-norsteroids.
Urinary samples collected from 0 to 120 h were subjected to methanolysis and beta-glucuronidase hydrolysis and were derivatized by N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) before gas chromatography-mass spectrometry analysis.
1(0,0,0,1) Details