Protein Information

Name AhR
Synonyms AHR; Ah receptor; AhR; Aryl hydrocarbon receptor; Aryl hydrocarbon receptor precursor; Dioxin receptor; Ah receptors; AhRs…

Compound Information

Name sulfuric acid
CAS sulfuric acid

Reference List

PubMed Abstract RScore(About this table)
12790202 Tsutsumi T, Amakura Y, Nakamura M, Brown DJ, Clark GC, Sasaki K, Toyoda M, Maitani T: Validation of the CALUX bioassay for the screening of PCDD/Fs and dioxin-like PCBs in retail fish. Analyst. 2003 May;128(5):486-92.

The sample extracts were cleaned up on a sulfuric acid-silica gel column followed by an activated carbon column, and the AhR activity of the separated PCDD/F and dioxin-like PCB fractions was determined using the assay.
82(1,1,1,2) Details
15051240 Asari M, Takatsuki H, Yamazaki M, Azuma T, Takigami H, Sakai S: Waste wood recycling as animal bedding and development of bio-monitoring tool using the CALUX assay. Environ Int. 2004 Jul;30(5):639-49.

The CALUX assay-determined TEQs (CALUX-TEQs) were significantly high in the sample after DMSO/n-hexane extraction, probably due to labile aryl hydrocarbon receptor (AhR) ligands such as PAHs; however, they were remarkably reduced through a single silica gel-44% sulfuric acid reflux treatment.
32(0,1,1,2) Details
16841312 Bittner M, Janosek J, Hilscherova K, Giesy J, Holoubek I, Blaha L: Activation of Ah receptor by pure humic acids. Environ Toxicol. 2006 Aug;21(4):338-42.

Organic extract of HA was further treated with sulfuric acid to study the role of possible trace persistent contaminants.
7(0,0,0,7) Details
16862773 Takigami H, Behnisch PA, Shiozaki K, Ohno M, Sakai SI: Bioassay monitoring of waste PCB samples during chemical destruction treatments. Water Sci Technol. 2006;53(11):43-50.


Dioxin-responsive chemical-activated luciferase expression (DR CALUX) AhR reporter gene bioassay was used to evaluate dioxin-like activity in the samples.
1(0,0,0,1) Details
16226227 Pollenz RS, Popat J, Dougherty EJ: Role of the carboxy-terminal transactivation domain and active transcription in the ligand-induced and ligand-independent degradation of the mouse Ahb-1 receptor. Biochem Pharmacol. 2005 Nov 25;70(11):1623-33. Epub 2005 Oct 13.


To assess the importance of transactivation domains (TAD), DNA binding and transcription on the degradation of the AH receptor (AHR), Hepa-1 cells were pre-treated with actinomycin D (AD) or cycloheximide (CHX) and exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD).
1(0,0,0,1) Details
16650473 Blaha L, Hilscherova K, Mazurova E, Hecker M, Jones PD, Newsted JL, Bradley PW, Gracia T, Duris Z, Horka I, Holoubek I, Giesy JP: Alteration of steroidogenesis in H295R cells by organic sediment contaminants and relationships to other endocrine disrupting effects. Environ Int. 2006 Aug;32(6):749-57. Epub 2006 May 2.

A similar pattern was observed with the extracts after treatment with concentrated sulfuric acid to remove labile chemicals (including PAHs) leaving only persistent PCBs, OCPs and potentially PCDD/Fs.
Comparison of the results with other mechanistically based bioassays (arylhydrocarbon receptor, AhR, mediated responses in H4IIE-luc cells, and estrogen receptor mediated effects in MVLN cells) revealed significant endocrine disrupting potencies of organic contaminants present in the sediments (most likely antiestrogenicity).
1(0,0,0,1) Details