Protein Information

Name Novozyme 188
Synonyms CBG; CBG; GLUC; CBGL 1; CBGL1; Cytosolic beta glucosidase; Cytosolic beta glucosidase like protein 1; GBA 3…

Compound Information

Name sulfuric acid
CAS sulfuric acid

Reference List

PubMed Abstract RScore(About this table)
18754081 Corredor DY, Salazar JM, Hohn KL, Bean S, Bean B, Wang D: Evaluation and characterization of forage Sorghum as feedstock for fermentable sugar production. Appl Biochem Biotechnol. 2009 Jul;158(1):164-79. Epub 2008 Aug 27.

Up to 72% hexose yield and 94% pentose yield were obtained using modified steam explosion with 2% sulfuric acid at 140 degrees C for 30 min and enzymatic hydrolysis with cellulase (15 filter per unit (FPU)/g cellulose) and beta-glucosidase (50 cellobiose units (CBU)/g cellulose).
31(0,1,1,1) Details
18478406 Zheng Y, Pan Z, Zhang R, Labavitch JM, Wang D, Teter SA, Jenkins BM: Evaluation of different biomass materials as feedstock for fermentable sugar production. Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):423-35.

Each of the biomass materials was first treated with dilute sulfuric acid under selected conditions (acid concentration =1.4% (w/w), temperature =165 degrees C, and time =8 min) and then treated with the enzymes (cellulases and beta-glucosidase).
6(0,0,1,1) Details
19170246 Kumar R, Wyman CE: Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies. Biotechnol Bioeng. 2009 Apr 15;102(6):1544-57.

Bovine serum albumin (BSA), Tween-20, and polyethylene glycol (PEG6000) were added to washed corn stover solids produced by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), dilute sulfuric acid (DA), lime, controlled pH, and sulfur dioxide (SO (2)) pretreatments and to untreated corn stover (UT) and pure Avicel glucan prior to adding cellulase supplemented with beta-glucosidase at an activity ratio of 1:2/g and a moderate enzyme loading of 16.1 mg/g glucan in the raw corn stover.
6(0,0,1,1) Details
16673419 Yang B, Wyman CE: BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates. Biotechnol Bioeng. 2006 Jul 5;94(4):611-7.

Cellulase and bovine serum albumin (BSA) were added to Avicel cellulose and solids containing 56% cellulose and 28% lignin from dilute sulfuric acid pretreatment of corn stover.
Adding a 1% concentration of BSA to dilute acid pretreated corn stover prior to enzyme addition at 15 FPU/g cellulose enhanced filter paper activity in solution by about a factor of 2 and beta-glucosidase activity in solution by about a factor of 14.
3(0,0,0,3) Details
19301243 Kumar R, Wyman CE: Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies. Biotechnol Prog. 2009 Mar-Apr;25(2):302-14.

Sugar solubilization was measured for times of up to 72 h using cellulase supplemented with beta-glucosidase at an activity ratio of 1:2, respectively, at combined protein mass loadings of 5.8-116 mg/g of glucan in poplar wood prior to pretreatment.
All pretreated poplar solids required high protein loadings to realize good sugar yields via enzymatic hydrolysis, and performance tended to be better for low pH pretreatments by dilute sulfuric acid and sulfur dioxide, possibly due to higher xylose removal.
2(0,0,0,2) Details
14585620 Wen Z, Liao W, Chen S: Hydrolysis of animal manure lignocellulosics for reducing sugar production. Bioresour Technol. 2004 Jan;91(1):31-9.

When raw dairy manure was pre-treated with 3% sulfuric acid at 110 degrees C for 1 h, hemicellulose was completely degraded into mainly arabinose, galactose and xylose.
The pretreated materials were then treated with cellulolytic enzymes, Celluclast-1.5L and Novozyme-188, to hydrolyze the cellulose.
2(0,0,0,2) Details
16575564 Katahira S, Mizuike A, Fukuda H, Kondo A: Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilating yeast strain. Appl Microbiol Biotechnol. 2006 Oct;72(6):1136-43. Epub 2006 Mar 31.

The sulfuric acid hydrolysate of lignocellulosic biomass, such as wood chips, from the forest industry is an important material for fuel bioethanol production.
In this study, we constructed a recombinant yeast strain that can ferment xylose and cellooligosaccharides by integrating genes for the intercellular expressions of xylose reductase and xylitol dehydrogenase from Pichia stipitis, and xylulokinase from Saccharomyces cerevisiae and a gene for displaying beta-glucosidase from Aspergillus acleatus on the cell surface.
1(0,0,0,1) Details
20202834 Digman MF, Shinners KJ, Casler MD, Dien BS, Hatfield RD, Jung HJ, Muck RE, Weimer PJ: Optimizing on-farm pretreatment of perennial grasses for fuel ethanol production. Bioresour Technol. 2010 Mar 2.

Switchgrass (Panicum virgatum L.) and reed canarygrass (Phalaris arundinacea L.) were pretreated under ambient temperature and pressure with sulfuric acid and calcium hydroxide in separate experiments.
Pretreated and untreated samples were fermented to ethanol by Saccharomyces cerevisiae D5A in the presence of a commercially available cellulase (Celluclast 1.5L) and beta-glucosidase (Novozyme 188).
1(0,0,0,1) Details
15932261 Saha BC, Iten LB, Cotta MA, Wu YV: Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol. Biotechnol Prog. 2005 May-Jun;21(3):816-22.


The maximum yield of monomeric sugars from rice hulls (15%, w/v) by dilute H2SO4 (1.0%, v/v) pretreatment and enzymatic saccharification (45 degrees C, pH 5.0, 72 h) using cellulase, beta-glucosidase, xylanase, esterase, and Tween 20 was 287 +/- 3 mg/g (60% yield based on total carbohydrate content).
1(0,0,0,1) Details
19119005 Zhu JY, Pan XJ, Wang GS, Gleisner R: Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresour Technol. 2009 Apr;100(8):2411-8. Epub 2008 Dec 31.

The results indicated that after the SPORL pretreatment of spruce chips with 8-10% bisulfite and 1.8-3.7% sulfuric acid on oven dry (od) wood at 180 degrees C for 30 min, more than 90% cellulose conversion of substrate was achieved with enzyme loading of about 14.6 FPU cellulase plus 22.5 CBU beta-glucosidase per gram of od substrate after 48 h hydrolysis.
81(1,1,1,1) Details
18781688 Kumar R, Wyman CE: Effect of enzyme supplementation at moderate cellulase loadings on initial glucose and xylose release from corn stover solids pretreated by leading technologies. Biotechnol Bioeng. 2009 Feb 1;102(2):457-67.

Moderate loadings of cellulase enzyme supplemented with beta-glucosidase were applied to solids produced by ammonia fiber expansion (AFEX), ammonia recycle (ARP), controlled pH, dilute sulfuric acid, lime, and sulfur dioxide pretreatments to better understand factors that control glucose and xylose release following 24, 48, and 72 h of hydrolysis and define promising routes to reducing enzyme demands.
33(0,1,1,3) Details