Protein Information

Name N acetyltransferase
Synonyms AAC 2; NAT2; AAC2; ARY 2; ARY2; Arylamide acetylase 2; Arylamine N acetyltransferase 2; N acetyltransferase…

Compound Information

Name glyphosate
CAS

Reference List

PubMed Abstract RScore(About this table)
17272278 Siehl DL, Castle LA, Gorton R, Keenan RJ: The molecular basis of glyphosate resistance by an optimized microbial acetyltransferase. J Biol Chem. 2007 Apr 13;282(15):11446-55. Epub 2007 Feb 1.

GAT is an N-acetyltransferase from Bacillus licheniformis that was optimized by gene shuffling for acetylation of the broad spectrum herbicide, glyphosate, forming the basis of a novel mechanism of glyphosate tolerance in transgenic plants (Castle, L.
32(0,1,1,2) Details
16621678 Johannes TW, Zhao H: Directed evolution of enzymes and biosynthetic pathways. Curr Opin Microbiol. 2006 Jun;9(3):261-7. Epub 2006 Apr 18.

Recent advances have focused on applying directed evolution to a variety of enzymes, such as epoxide hydrolase, glyphosate N-acetyltransferase, xylanase and phosphotriesterase, in order to improve their activity, selectivity, stability and solubility.
6(0,0,1,1) Details
15155947 Castle LA, Siehl DL, Gorton R, Patten PA, Chen YH, Bertain S, Cho HJ, Duck N, Wong J, Liu D, Lassner MW: Discovery and directed evolution of a glyphosate tolerance gene. Science. 2004 May 21;304(5674):1151-4.

We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity.
6(0,0,1,1) Details
16547651 Tan S, Evans R, Singh B: Herbicidal inhibitors of amino acid biosynthesis and herbicide-tolerant crops. Amino Acids. 2006 Mar;30(2):195-204. Epub 2006 Mar 20.

Similarly, bar and pat genes isolated from Streptomyces hygroscopicus and S. viridochromogenes, respectively, have been inserted into plants to encode phosphinothricin N-acetyltransferase to detoxify glufosinate.
Glyphosate affects aromatic amino acid biosynthesis by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
1(0,0,0,1) Details