Protein Information

Name C18
Synonyms B1; D1; BBS 9; BBS9; BBS9 GENE; Bardet Biedl syndrome 9 protein; C18; PTH responsive osteosarcoma B1 protein…

Compound Information

Name atrazine
CAS

Reference List

PubMed Abstract RScore(About this table)
10720259 Pinto GM, Jardim IC: Use of solid-phase extraction and high-performance liquid chromatography for the determination of triazine residues in water: validation of the method. J Chromatogr A. 2000 Feb 11;869(1-2):463-9.

The method involves concentration with C18 solid-phase extraction cartridges followed by high-performance liquid chromatographic analysis using a C18 column with UV detection at 230 nm, a mobile phase of methanol-water (60:40, v/v) at pH 4.6 (phosphoric acid) and a flow-rate of 0.8 ml/min.
The method can be used for determination of atrazine, simazine, cyanazine and ametryn in water, within the international limits of 0.1 microg/l.
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10076835 Onnerfjord P, Eremin SA, Emneus J, Marko-Varga G: A flow immunoassay for studies of human exposure and toxicity in biological samples. J Mol Recognit. 1998 Winter;11(1-6):182-4.

The column traps the free unbound tracer (Ag*) in its hydrophobic (C18) inner cavity but excludes the large Ab-Ag* complex, which is passed on and measured by the fluorescence detector.
The flow immunoassay was developed using the triazine herbicide atrazine as a model compound owing to its human toxicity and widespread use.
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11013729 Hernandez F, Hidalgo C, Sancho JV, Lopez FJ: Coupled-column liquid chromatography applied to the trace-level determination of triazine herbicides and some of their metabolites in water samples. Anal Chem. 1998 Aug 1;70(15):3322-8.

First, a completely automated liquid chromatographic method based on on-line trace enrichment in a prepacked precolumn and using diode array detection has been developed for the simultaneous trace-level determination of six triazine herbicides (simazine, cyanazine, atrazine, terbumeton, terbuthylazine, and terbutryn) and the main atrazine metabolites (desisopropylatrazine, desethylatrazine, and hydroxyatrazine).
After preconcentration parameters were optimized by testing two different sorbents (C18 and PRP-1) in three cartridges with different dimensions, a sample volume of 100 mL was selected in order to achieve maximal solute preconcentration.
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11990989 Carabias-Martinez R, Rodriguez-Gonzalo E, Herrero-Hernandez E, Sanchez-San RF, Prado Flores MG: Determination of herbicides and metabolites by solid-phase extraction and liquid chromatography evaluation of pollution due to herbicides in surface and groundwaters. J Chromatogr A. 2002 Mar 15;950(1-2):157-66.

The compounds studied were atrazine and its metabolites, desethylatrazine, desethyldesisopropylatrazine (DEDIA), 2-hydroxyatrazine, desethyl-2-hydroxyatrazine and desisopropyl-2-hydroxyatrazine (DIHA), terbutryne and its metabolite 2-hydroxyterbutylazine, and chlorotoluron and its metabolite 3-chloro-4-methylphenylurea.
In the SPE preconcentration step, different types of sorbent were studied: C18 on silica and polymeric sorbents (Oasis and LiChrolut EN), the best results being obtained with the styrene-divinylbenzene cartridge and when the elution was performed with methanol and ethyl acetate.
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12831194 Queiroz SC, Melo LF, Jardim IC: Poly (methyloctadecylsiloxane) immobilized on silica by gamma radiation for use in solid-phase extraction. J Chromatogr A. 2002 Mar 1;948(1-2):171-6.

This work presents a fast, easy and effective procedure to obtain silica-based C18 type sorbents for use in solid-phase extraction (SPE).
Water samples spiked with a mixture of pesticides (benomyl, tebuthiuron, diuron, simazine, atrazine and ametryn) were extracted in the off-line mode in order to evaluate the performance of this sorbent.
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11938923 Liu F, Mu W, Wang J, Yu J: [Study on the analytical method of s-triazine herbicide residues in soil and maize]. Se Pu. 1998 Nov;16(6):543-4.

After filtration, the extract was defatted with petroleum ether and then it was passed through a short C18 column for pretreatment and analysed by Nova-Pak C18 column, 150 mm x 3.9 mm i.d., with V (MeOH): V (H2O) = 55:45 as mobile phase.
The recoveries were 82.4%-99.8% for cyanazine, 85.6%-102.3% for atrazine and 89.1%-108.4% for simetryne.
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18644603 Gervais G, Brosillon S, Laplanche A, Helen C: Ultra-pressure liquid chromatography-electrospray tandem mass spectrometry for multiresidue determination of pesticides in water. J Chromatogr A. 2008 Aug 22;1202(2):163-72. Epub 2008 Jul 9.

The selected pesticides represent a broad range of polarity and volatility [benzoylcyclohexanedione (mesotrione and sulcotrione); chloroacetamide (acetochlor, alachlor, dimethenamide, and metolachlor); phenoxyacetic acid (2,4-D and MCPA); phenoxypropionic (dichloprop and mecoprop); phenylurea (chlortoluron, diuron, isoproturon, linuron, and metoxuron); sulfonylurea (foramsulfuron, iodosulfuron, and nicolsulfuron); triazine (atrazine, cyanazine, desethylatrazine (DEA), desisopropylatrazine (DIA), simazine, and terbutylazine)].
The separation was carried out on an acquity UPLC BEH C18 column (1.7 microm, 50 mm x 1 mm ID) using a gradient elution profile and mobile phase consisting of 0.1% formic acid in water and acetonitrile.
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16323791 Stephens BS, Kapernick A, Eaglesham G, Mueller J: Aquatic passive sampling of herbicides on naked particle loaded membranes: accelerated measurement and empirical estimation of kinetic parameters. Environ Sci Technol. 2005 Nov 15;39(22):8891-7.

Water-sampler equilibrium partitioning coefficients and aqueous boundary layer mass transfer coefficients for atrazine, diuron, hexazionone and fluometuron onto C18 and SDB-RPS Empore disk-based aquatic passive samplers have been determined experimentally under a laminar flow regime (Re = 5400).
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8360301 Balinova A: Solid-phase extraction followed by high-performance liquid chromatographic analysis for monitoring herbicides in drinking water. J Chromatogr. 1993 Jul 23;643(1-2):203-7.

Bentazone, 2,4-D, MCPA, fluazifop-acid, metoxuron, monolinuron, metobromuron, diuron, linuron, atrazine and simazine were determined simultaneously in a single run on a C18 HPLC column.
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12867196 Ma WT, Fu KK, Cai Z, Jiang GB: Gas chromatography/mass spectrometry applied for the analysis of triazine herbicides in environmental waters. Chemosphere. 2003 Sep;52(9):1627-32.

Gas chromatography coupled with mass spectrometry (GC/MS) was used to analyze simazine, atrazine (ATR), cyanazine, as well as the degradation products of ATR such as deethylatrazine and deisopropylatrazine in environmental water samples.
Recovery of ATR by the SPE pre-concentration using a C18 cartridge was determined as 90.5 +/- 3.5%.
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12433116 Dopico MS, Gonzalez MV, Castro JM, Gonzalez E, Perez J, Rodriguez M, Calleja A: Determination of triazines in water samples by high-performance liquid chromatography with diode-array detection. J Chromatogr Sci. 2002 Oct;40(9):523-8.

In this study, carbonaceous and polymeric adsorbents are compared with C18 for the solid-phase extraction of simazine, atrazine, and propazine in water samples in order to quantitate their levels by high-performance liquid chromatography using photodiode-array detection.
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11128134 Mueller TC, Senseman SA, Wauchope RD, Clegg C, Young RW, Southwick LM, Riley MB, Moye HA, Dumas JA, Mersie W, Mattice JD, Leidy RB: Recovery of atrazine, bromacil, chlorpyrifos, and metolachlor from water samples after concentration on solid-phase extraction disks: interlaboratory study. J AOAC Int. 2000 Nov-Dec;83(6):1327-33.

An interlaboratory comparison was conducted in 1997 and 1998 to examine the feasibility of using C18 solid-phase extraction disks (Empore) to simultaneously determine the herbicides atrazine, bromacil, and metolachlor and the insecticide chlorpyrifos in water samples.
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12187961 Lambropoulou DA, Sakkas VA, Hela DG, Albanis TA: Application of solid-phase microextraction in the monitoring of priority pesticides in the Kalamas River (N.W. J Chromatogr A. 2002 Jul 19;963(1-2):107-16.

A comparison with a well-established solid-phase extraction (C18 disks) procedure was performed for samples of high-season application (May-September) in order to confirm the effectiveness of the SPME technique.
The most frequently detected pesticides were some of the more commonly used herbicides, such as S-ethyl-N,N-di-n-propylthiol carbamate (EPTC), trifluralin, atrazine, deethylatrazine, terbuthylazine and alachlor, and insecticides, such as carbofuran, diazinon, disulfoton, parathion methyl, parathion ethyl, fenthion and ethion.
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15065776 Melo LF, Collins CH, Jardim IC: New materials for solid-phase extraction and multiclass high-performance liquid chromatographic analysis of pesticides in grapes. J Chromatogr A. 2004 Apr 2;1032(1-2):51-8.

Sample preparation procedures which included the use of new aminopropyl (NH2) and octadecyl (C18) solid-phase extraction (SPE) sorbents are proposed for the simultaneous multiclass determination of the fungicide benomyl and of the herbicides tebuthiuron, diuron, simazine, atrazine, and ametryn in grapes, using single wavelength high-performance liquid chromatography.
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8025630 Psathaki M, Manoussaridou E, Stephanou EG: Determination of organophosphorus and triazine pesticides in ground- and drinking water by solid-phase extraction and gas chromatography with nitrogen-phosphorus or mass spectrometric detection. J Chromatogr A. 1994 Apr 29;667(1-2):241-8.

Trace enrichment and determination of ethoprophos, fenamiphos, fenthion, isophenphos, mevinphos, monocrotophos, atrazine and simazine were performed by solid-phase extraction on XAD-2 columns and Sep-Pak C18 cartridges, subsequent elution with an organic solvent and determination by GC with nitrogen-phosphorus detection (NPD) and mass spectrometry in the selected-ion monitoring mode (MS-SIM).
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10870695 Jeannot R, Sabik H, Sauvard E, Genin E: Application of liquid chromatography with mass spectrometry combined with photodiode array detection and tandem mass spectrometry for monitoring pesticides in surface waters. J Chromatogr A. 2000 May 19;879(1):51-71.

Different extraction techniques were employed, including liquid-liquid extraction with dichloromethane, and solid-phase extraction using C18-bonded silica and graphitized carbon black cartridges.
Eleven of the target chemicals (desethylatrazine, desisopropylatrazine, atrazine, simazine, terbuthylazine, metolachlor, carbendazime, bentazone, penconazole, diuron and isoproturon) were detected by LC-MS at concentrations ranging from 20 to 900 ng/l in the surface waters from France, and six pesticides (atrazine, desethylatrazine, desisopropylatrazine, cyanazine, simazine and metolachlor) were detected by LC-MS and LC-MS-MS at concentrations ranging from 3 to 52 ng/l in the samples drawn from the St.
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18980142 Tuzimski T: Determination of pesticides in water samples from the Wieprz-krzna Canal in the Leczynsko-Wlodawskie Lake District of southeastern Poland by thin-layer chromatography with diode array scanning and high-performance column liquid chromatography with diode array detection. J AOAC Int. 2008 Sep-Oct;91(5):1203-9.

Atrazine, clofentezine, chlorfenvinphos, hexaflumuron, terbuthylazine, lenacyl, neburon, bitertanol, and metamitron were enriched from canal water samples by solid-phase extraction (SPE) on octadecyl silane (C18)/styrene-divinylbenzene-1, C18, C18 Polar Plus, and cyanopropyl (CN) cartridges.
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18371835 El-Sheikh AH, Sweileh JA, Al-Degs YS, Insisi AA, Al-Rabady N: Critical evaluation and comparison of enrichment efficiency of multi-walled carbon nanotubes, C18 silica and activated carbon towards some pesticides from environmental waters. Talanta. 2008 Feb 15;74(5):1675-80. Epub 2007 Sep 15.

In this work, optimization of multi-residue solid phase extraction (SPE) procedures coupled with high-performance liquid chromatography for the determination of Propoxur, Atrazine and Methidathion from environmental waters is reported.
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12477195 Mersie W, Clegg C, Wauchope RD, Dumas JA, Leidy RB, Riley MB, Young RW, Mattice JD, Mueller TC, Senseman SA: Interlaboratory comparison of pesticide recovery from water using solid-phase extraction disks and gas chromatography. J AOAC Int. 2002 Nov-Dec;85(6):1324-30.

An interlaboratory study was conducted to assess the suitability of C18 solid-phase extraction disks to retain and ship different pesticides from water samples.
The highest recovery from deionized or surface water using shipped disks was obtained for cyanazine (> 97%), followed by metalaxyl (> 96%), and atrazine (> 92%).
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8997737 Ting KC, Tamashiro GS: Off-line high-performance liquid chromatography and solid-phase extraction clean-up for confirmation of pesticide residues in fresh produce by gas chromatography-mass spectrometry. J Chromatogr A. 1996 Nov 22;754(1-2):455-62.


In this research, a combination of HPLC/C18 and SPE/Florisil clean-up methods were used to reduce matrices in 10 commodities.
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12406605 Hamada M, Wintersteiger R: Rapid screening of triazines and quantitative determination in drinking water. J Biochem Biophys Methods. 2002 Oct-Nov;53(1-3):229-39.

The method was also applied to other triazine herbicides such as ametryn, atrazine, propazine, terbuthylazine and simazine.
Drinking water samples spiked with triazines were extracted using RP-C18 polar plus cartridges, and the extract could be then dansylated as a total.
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17723718 Bruzzoniti MC, Sarzanini C, Costantino G, Fungi M: Determination of herbicides by solid phase extraction gas chromatography-mass spectrometry in drinking waters. Anal Chim Acta. 2006 Sep 25;578(2):241-9. Epub 2006 Jun 30.

A solid phase extraction (SPE) method has been optimized for the gas chromatography-mass spectrometry (GC-MS) simultaneous determination of herbicides belonging to the following different families: carbamate (molinate), atrazines (atrazine, propazine, simazine, ametryne, cyanazine, terbutylazine, deethylterbutylazine, deethylatrazine), dinitroaniline (trifluralin, pendimethalin), chloroacetamide (alachlor, metolachlor).
Different solid substrates have been compared (C18, cyano, styrene-divinylbenzene, phenyl, graphitic carbon).
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9299733 Ferrer I, Barcelo D: Stability of pesticides stored on polymeric solid-phase extraction cartridges. J Chromatogr A. 1997 Aug 22;778(1-2):161-70.


Fonofos was stable when compared to their storage in C18 precolumns.
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16915825 Cobb JM, Mattice JD, Senseman SA, Dumas JA, Mersie W, Riley MB, Potter TL, Mueller TC, Watson EB: Stability of pesticides on solid-phase extraction disks after incubation at various temperatures and for various time intervals: interlaboratory study. J AOAC Int. 2006 Jul-Aug;89(4):903-12.

Deionized water fortified with selected pesticides was extracted by using 2 types of SPE filtration disks (Empore C18 and Speedisk C18XF), and after extraction, the disks were incubated at 3 temperatures (25, 40, and 55 degrees C) and for 2 time intervals (4 and 14 days).
Deionized water was fortified with atrazine, carbofuran, and chlorpyrifos by all participating laboratories.
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10693004 Penuela GA, Barcelo D: Comparative photodegradation study of atrazine and desethylatrazine in water samples containing titanium dioxide/hydrogen peroxide and ferric chloride/hydrogen peroxide. J AOAC Int. 2000 Jan-Feb;83(1):53-60.

After irradiation, the water samples containing the spiked pesticides were preconcentrated by using C18 solid-phase extraction disks and analyzed by gas chromatography with nitrogen-phosphorus and mass spectrometric detection.
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9435119 McLaughlin RA, Johnson BS: Optimizing recoveries of two chlorotriazine herbicide metabolites and 11 pesticides from aqueous samples using solid-phase extraction and gas chromatography-mass spectrometry. J Chromatogr A. 1997 Nov 28;790(1-2):161-7.


Two C18 phases in cartridge format were compared and recoveries were found to be highly sensitive to sorbent amount, sample volume and presence of parent compounds.
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3584389 Ferris IG, Haigh BM: A rapid and sensitive HPLC procedure for the determination of atrazine residues in soil-water extracts. J Chromatogr Sci. 1987 Apr;25(4):170-3.

The system consists of an autosampler connected to a C18 precolumn linked via a switching valve to a C18 analytical column and spectrophotometer.
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15909507 Melo LF, Collins CH, Jardim IC: High-performance liquid chromatographic determination of pesticides in tomatoes using laboratory-made NH2 and C18 solid-phase extraction materials. J Chromatogr A. 2005 May 6;1073(1-2):75-81.

The SPE materials were obtained by thermal immobilization of appropriate polysiloxanes onto 40 microm silica surfaces and were used in sample preparation for multiresidue analysis of the following pesticides: tebuthiuron and diuron (urea herbicides), simazine, atrazine and ametryn (triazines herbicides) and benomyl (benzimidazol fungicide).
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2376549 Nash RG: Solid-phase extraction of carbofuran, atrazine, simazine, alachlor, and cyanazine from shallow well water. J Assoc Off Anal Chem. 1990 May-Jun;73(3):438-42.

Extraction of several nitrogen-containing pesticides from water on solid-phase C18 cartridges was rapid and accurate.
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18020313 Jornet D, Gonzalez-Martinez MA, Maquieira A, Puchades R: Advanced homogeneous-heterogeneous immunosensing format employing restricted access supports. Anal Chem. 2007 Dec 15;79(24):9331-9. Epub 2007 Nov 17.

The immunosensor is based on the homogeneous competition among the analyte, a fluorescent tracer, and the antibody, followed by separation of free and bound species by means of a restricted access alkyl-diol silica C18 reversed-phase chromatographic support.
The immunosensor principle is demonstrated by determining atrazine in a completely automated manner at 2 min/sample without regeneration of the support and a limit of detection of 1.0 microg/L with the optimized system.
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7580319 Holland DC, Munns RK, Roybal JE, Hurlbut JA, Long AR: Liquid chromatographic determination of simazine, atrazine, and propazine residues in catfish. J AOAC Int. 1995 Jul-Aug;78(4):1067-71.

The herbicides are extracted from catfish homogenates with ethyl acetate, followed by solvent partitioning between acetonitrile and petroleum ether and additional cleanup on a C18 cartridge.
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15387168 Gatidou G, Zhou JL, Thomaidis NS: Microwave-assisted extraction of Irgarol 1051 and its main degradation product from marine sediments using water as the extractant followed by gas chromatography-mass spectrometry determination. J Chromatogr A. 2004 Aug 13;1046(1-2):41-8.

The extraction procedure was followed by solid-phase extraction (SPE) on reverse phase C18 cartridges.
Quantification was performed with matrix-matched calibration using atrazine-d5 as internal standard.
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16393901 Kotrikla A, Gatidou G, Lekkas TD: Monitoring of triazine and phenylurea herbicides in the surface waters of Greece. J Environ Sci Health B. 2006;41(2):135-44.

Samples from 10 rivers and 7 lakes were analyzed for the presence of five triazine (atrazine, cyanazine, prometryne, simazine, terbuthylazine) and five phenylurea (chlorotoluron, diuron, linuron, metobromuron, monolinuron) herbicides.
The samples were extracted with C18 cartridges and analyzed by high-performance liquid chromatography-diode array detection (HPLC-DAD).
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9271068 Martin-Esteban A, Fernandez P, Camera C: Baker's yeast biomass (Saccharomyces cerevisae) for selective on-line trace enrichment and liquid chromatography of polar pesticides in water. Anal Chem. 1997 Aug 15;69(16):3267-71.

Baker's yeast cells (Saccharomices cerevisae) were successfully immobilized onto silica gel and used in the on-line isolation and trace enrichment of desisopropylatrazine, desethylatrazine, hydroxyatrazine, simazine, cyanazine, atrazine, carbaryl, propanil, linuron, and fenamiphos.
The degree of selectivity was evaluated by comparing the chromatograms obtained after on-line sample preconcentration on the yeast precolumn with those obtained by on-line solid-phase extraction using a precolumn filled with C18 material.
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10749506 Turiel E, Fernandez P, Perez-Conde C, Camara C: Trace-level determination of triazines and several degradation products in environmental waters by disk solid-phase extraction and micellar electrokinetic chromatography. J Chromatogr A. 2000 Mar 3;872(1-2):299-307.

An analytical method combining disk solid-phase extraction with micellar electrokinetic chromatography has been developed for the determination of atrazine, simazine, hydroxyatrazine, deisopropylatrazine, deethylatrazine, propazine and prometryn in water samples.
C18-bonded silica and poly (styrene-divinylbenzene) (PS-DVB) disks were evaluated for solid-phase extraction of the selected pesticides (11 of water sample).
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16797563 Chafer-Pericas C, Herraez-Hernandez R, Campins-Falco P: On-fibre solid-phase microextraction coupled to conventional liquid chromatography versus in-tube solid-phase microextraction coupled to capillary liquid chromatography for the screening analysis of triazines in water samples. J Chromatogr A. 2006 Sep 1;1125(2):159-71. Epub 2006 Jun 23.

Atrazine, simazine, propazine, ametryn, prometryn and terbutryn were selected as model compounds.
The in-tube SPME approach with a C18 packed column (35 mm x 0.5 mm I.D., 5 microm particle size) connected to a switching micro-valve provided the best sensitivity; under such configuration the LODs were between 0.025 and 0.5 microg/L.
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19071262 D'Archivio AA, Fanelli M, Mazzeo P, Ruggieri F: Comparison of different sorbents for multiresidue solid-phase extraction of 16 pesticides from groundwater coupled with high-performance liquid chromatography. Talanta. 2007 Jan 15;71(1):25-30. Epub 2006 Apr 18.

The compounds analysed were: aldicarb, atrazine, desethylatrazine, desysopropylatrazine, carbofuran, 2,4-D, dicloran, fenitrothion, iprodione, linuron, metalaxyl, metazachlor, phenmedipham, procymidone, simazine and vinclozolin.
Five different SPE sorbents, C (18) bonded silica (Isolute SPE C18 (EC)), graphitised carbon black (Superclean Envi-Carb), highly cross-linked polystyrene-divinylbenzene (Lichrolut EN), divinylbenzene-N-vinylpyrrolidone (Oasis HLB) and surface modified styrene-divinylbenzene (Strata X), were compared.
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9297841 Hogenboom AC, Speksnijder P, Vreeken RJ, Niessen WM, Brinkman UA: Rapid target analysis of microcontaminants in water by on-line single-short-column liquid chromatography combined with atmospheric pressure chemical ionization tandem mass spectrometry. J Chromatogr A. 1997 Aug 8;777(1):81-90.

The applicability of trace enrichment and separation of microcontaminants on a 10 mm x 2 mm I.D. high-pressure packed (8 microns C18 bonded silica or 10-15 microns PLRP-S) column combined on-line with an atmospheric pressure chemical ionization MS-MS system is demonstrated for the target analysis of herbicides in river water.
Target compound analyses of river water allowed the confirmation of the presence of herbicides such as diuron, simazine, atrazine and terbutylazine at sub-microgram/l levels.
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