Protein Information

Name tyrosine hydroxylase
Synonyms Putative tyrosine hydroxylase variant; TH; TH protein; TYH; Truncated tyrosine hydroxylase; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine hydroxylase…

Compound Information

Name chloralose
CAS

Reference List

PubMed Abstract RScore(About this table)
12088752 Nosjean A, Callera JC, Bonagamba L, Machado B, Hamon M, Laguzzi R: Serotonin (3) receptor stimulation in the nucleus tractus solitarii activates non-catecholaminergic neurons in the rat ventrolateral medulla. Neuroscience. 2002;112(4):935-49.

For this purpose, double immunohistochemical labeling for tyrosine hydroxylase and c-fos protein was performed all along the ventrolateral medulla after microinjection of 1-(m-chlorophenyl)-biguanide, a selective and potent 5-HT (3) receptor agonist, into the nucleus tractus solitarii of alpha-chloralose/urethane-anaesthetized rats.
37(0,1,2,2) Details
9373011 Schreihofer AM, Guyenet PG: Identification of C1 presympathetic neurons in rat rostral ventrolateral medulla by juxtacellular labeling in vivo. J Comp Neurol. 1997 Nov 3;387(4):524-36.

We used a juxtacellular labeling technique to individually fill spontaneously active, barosensitive, bulbospinal RVLM neurons with biotinamide following electrophysiological characterization in chloralose-anesthetized rats.
To determine whether these neurons could be classified as C1 cells, the biotinamide-labeled cells were processed for detection of tyrosine hydroxylase.
2(0,0,0,2) Details
8653425 Gatti PJ, Homby PJ, Mandal AK, Norman WP, DaSilva AM, Gillis RA: Cardiovascular neurons in cat caudal ventrolateral medulla: location and characterization of GABAergic input. Brain Res. 1995 Sep 25;693(1-2):80-7.

This was done by microinjecting GABA receptor antagonists and agonists, and a negative allosteric modulator of the GABA receptor, namely, ethyl-beta-carboline-3-carboxylate, into the caudal ventrolateral medulla of alpha-chloralose-anesthetized animals while monitoring arterial blood pressure and heart rate.
Localization studies where performed relating injection sites in the caudal ventrolateral medulla where cardiovascular responses were elicited, to neurons exhibiting immunoreactivity to tyrosine hydroxylase (TH) and phenethyl-N-methyl-transferase (PNMT).
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11049859 Schreihofer AM, Guyenet PG: Role of presympathetic C1 neurons in the sympatholytic and hypotensive effects of clonidine in rats. Am J Physiol Regul Integr Comp Physiol. 2000 Nov;279(5):R1753-62.

In chloralose-anesthetized and ventilated rats, clonidine (10 microg/kg iv) decreased arterial pressure (116 +/- 6 to 84 +/- 2 mmHg) and splanchnic nerve activity (93 +/- 3% from baseline).
Extracellular recording and juxtacellular labeling of barosensitive bulbospinal RVLM neurons revealed that most cells were inhibited by clonidine (26/28) regardless of phenotype [tyrosine hydroxylase (TH)-immunoreactive cells: 48 +/- 7%; non-TH-immunoreactive cells: 42 +/- 5%], although the inhibition of most neurons was modest compared with the observed sympathoinhibition.
1(0,0,0,1) Details
12612005 Schreihofer AM, Guyenet PG: Baro-activated neurons with pulse-modulated activity in the rat caudal ventrolateral medulla express GAD67 mRNA. J Neurophysiol. 2003 Mar;89(3):1265-77. Epub 2002 Nov 20.


We recorded extracellularly from 19 baro-activated, pulse-modulated CVLM neurons in chloralose-anesthetized rats.
0(0,0,0,0) Details