Protein Information

Name hexokinase
Synonyms Brain form hexokinase; HK1; Glycolytic enzyme; HK I; HK1 sa; HK1 sb; HK1 ta; HK1 tb…

Compound Information

Name sodium fluoride
CAS sodium fluoride (NaF)

Reference List

PubMed Abstract RScore(About this table)
11110164 Nishikimi A, Uekawa N, Yamada M: Involvement of glycolytic metabolism in developmental inhibition of rat two-cell embryos by phosphate. J Exp Zool. 2000 Dec 1;287(7):503-9.


Addition of an inhibitor of enolase in glycolysis, sodium fluoride (NaF), at 1 mM to the Glc (-) medium also enhanced the inhibitory effects of 10 microM phosphate, whereas 1 mM NaF in the absence of phosphate showed no inhibitory effects on the development of 2-cell embryos to morula and blastocyst stages.
0(0,0,0,0) Details
11867637 Thongboonkerd V, Luengpailin J, Cao J, Pierce WM, Cai J, Klein JB, Doyle RJ: Fluoride exposure attenuates expression of Streptococcus pyogenes virulence factors. J Biol Chem. 2002 May 10;277(19):16599-605. Epub 2002 Feb 26.

A 16-h exposure to sodium fluoride caused decreased expression of proteins required to respond to cellular stress, including anti-oxidants, glycolytic enzymes, transcriptional and translational regulators, and protein folding.
6(0,0,1,1) Details
19282354 Gambino R, Piscitelli J, Ackattupathil TA, Theriault JL, Andrin RD, Sanfilippo ML, Etienne M: Acidification of blood is superior to sodium fluoride alone as an inhibitor of glycolysis. Clin Chem. 2009 May;55(5):1019-21. Epub 2009 Mar 12.

We analyzed the aliquots in duplicate by means of a hexokinase-based enzymatic method.
1(0,0,0,1) Details
16183791 Otsuki S, Morshed SR, Chowdhury SA, Takayama F, Satoh T, Hashimoto K, Sugiyama K, Amano O, Yasui T, Yokote Y, Akahane K, Sakagami H: Possible link between glycolysis and apoptosis induced by sodium fluoride. . J Dent Res. 2005 Oct;84(10):919-23.

Since fluoride is known as an inhibitor of glycolytic enzymes, we investigated the possible connection between NaF-induced apoptosis and glycolysis in human promyelocytic leukemia HL-60 cells.
1(0,0,0,1) Details
17596547 Waring WS, Evans LE, Kirkpatrick CT: Glycolysis inhibitors negatively bias blood glucose measurements: potential impact on the reported prevalence of diabetes mellitus. J Clin Pathol. 2007 Jul;60(7):820-3.

One of these involves addition of glycolysis inhibitors, such as sodium fluoride-potassium oxalate (NaF-KOx) to sample collection tubes, whereas the other requires immediate refrigeration and sample separation.
Glucose concentrations were determined using an Olympus AU 2700 analyser incorporating an automated hexokinase method.
1(0,0,0,1) Details