Protein Information

Name ATPase
Synonyms ATP7A; MK; ATPase; Cation transporting ATPase; ATP7A protein; ATPase Cu(2+) transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1…

Compound Information

Name sodium fluoride
CAS sodium fluoride (NaF)

Reference List

PubMed Abstract RScore(About this table)
12930674 Miao Q, Liu BC, Zhang XC, You BR, Xu M, Kang N: [Cloning of differentially displayed cDNAs involved in NaF-treated osteoblasts]. Zhonghua Yu Fang Yi Xue Za Zhi. 2003 Jul;37(4):251-5.

METHODS: Rat osteoblasts were treated with 2.0 mmol/L of sodium fluoride (NaF) for two weeks in vitro, and difference in the gene expression between the NaF-treated and normal osteoblasts was compared with mRNA differential display (DD-PCR) technique.
RESULTS: Among the six differentially expressed gene fragments which had been cloned, expression of the ribosomal protein L5 gene, ATPase Na (+) K (+) transporting beta polypeptide 3 gene, karyopherin alpha 2 gene and cis-Golgi body p28 gene was lower and expression of ubiquitous-conjugating enzyme E2D 3 gene and a newly-discovered gene fragment in this study showed up-regulated in the NaF-treated osteoblasts of the rats.
1(0,0,0,1) Details
14659655 Hall IH, Schwab UE, Ward ES, Ives T: Disposition and intracellular levels of moxifloxacin in human THP-1 monocytes in unstimulated and stimulated conditions. Int J Antimicrob Agents. 2003 Dec;22(6):579-87.

The uptake of moxifloxacin was reduced by sodium fluoride, -azide, -cyanide, low temperature and low pH.
The sodium-ATPase pump when blocked, also retarded moxifloxacin uptake at 2 and 4 h.
1(0,0,0,1) Details
10323588 Fukao M, Hattori Y, Sato A, Liu MY, Watanabe H, Kim TQ, Kanno M: Relationship between NaF- and thapsigargin-induced endothelium-dependent hyperpolarization in rat mesenteric artery. Br J Pharmacol. 1999 Apr;126(7):1567-74.


The selective inhibitors of the Ca2+ -pump ATPase of endoplasmic reticulum, thapsigargin and cyclopiazonic acid, elicited hyperpolarization, whose properties were very similar to those of NaF.
1(0,0,0,1) Details
18450565 Voicu MC, Zwiazek JJ, Tyree MT: Light response of hydraulic conductance in bur oak (Quercus macrocarpa) leaves. Tree Physiol. 2008 Jul;28(7):1007-15.


Atrazine (a photosystem II inhibitor), fusicoccin (which stimulates plasma membrane-bound H (+)-ATPase) and HgCl (2) (an aquaporin blocker) reduced the light response of leaf lamina hydraulic conductance.
1(0,0,0,1) Details
9703990 Calaghan S, White E, Colyer J: Preservation of the in vivo phosphorylation status of phospholamban in the heart: evidence for a site-specific difference in the dephosphorylation of phospholamban. Biochem Biophys Res Commun. 1998 Jul 30;248(3):701-5.


The phosphorylation status of the cardiac sarcoplasmic reticular (SR) protein phospholamban determines the activity of the SR Ca (2+)-ATPase.
1(0,0,0,1) Details
8962792 Schetinger MR, Wyse AT, Da Silva LB, Barcellos CK, Dias RD, Sarkis JJ: Effects of aluminum chloride on the kinetics of rat cortex synaptosomal ATP diphosphohydrolase (EC 3.6.1.5). Biol Trace Elem Res. 1995 Dec;50(3):209-19.


The metal ion significantly inhibited ATPase and ADPase activities of the enzyme at all concentrations tested in vitro (0.01, 0.05, 0.5, 5, and 10 mM) in the presence of 1.5 mM calcium.
1(0,0,0,1) Details
12071426 Ekambaram P, Paul V: Modulation of fluoride toxicity in rats by calcium carbonate and by withdrawal of fluoride exposure. Pharmacol Toxicol. 2002 Feb;90(2):53-8.

In order to assess the effect of calcium on the toxic effects of fluoride, adult female Wistar rats were treated with sodium fluoride (NaF, 500 ppm in drinking water) alone or in combination with calcium carbonate (CaCO3, 50 mg/ kg by oral intubation) daily for 60 days.
Dental lesions, inhibition of the activities of AchE and N+ K+ ATPase and a decrease in the concentration of protein, and serum calcium were also observed in these animals.
1(0,0,0,1) Details
14629005 Alves-Ferreira M, Dutra PM, Lopes AH, Ferreira-Pereira A, Scofano HM, Meyer-Fernandes JR: Magnesium-dependent ecto-ATP diphosphohydrolase activity in Herpetomonas muscarum muscarum. Curr Microbiol. 2003 Oct;47(4):265-71.

Classical inhibitors of acid phosphatases, such as sodium orthovanadate (NaVO3), sodium fluoride (NaF), and ammonium molybdate presented no effect on this activity.
The ecto-ATPase activity was insensitive to oligomycin and sodium azide, two inhibitors of mitochondrial Mg-ATPase, bafilomycin A1, a V-ATPase inhibitor, ouabain, a Na (+)+K+-ATPase inhibitor and to levamizole, an inhibitor of alkaline phosphatase.
1(0,0,0,1) Details
8932515 Anand RJ, Kanwar U, Sanyal SN: Transport of glycine in the brush border and basal cell membrane vesicles of the human term placenta. Biochem Mol Biol Int. 1996 Feb;38(1):21-30.


Nicotine, insulin, sodium fluoride and sodium arsenate were inhibitors for both the vesicles.
0(0,0,0,0) Details
12498919 Adeagbo AS, Patel D, Iddrissu A, Walker J, Thirumalai S, Joshua IG, Schuschke D, Wang Y: NS-398, a selective cyclooxygenase-2 blocker, acutely inhibits receptor-mediated contractions of rat aorta: role of endothelium. Eur J Pharmacol. 2003 Jan 1;458(1-2):145-54.


NS-398 altered contractions to these receptor agonists in denuded rings only at 10 microM, and did not significantly alter contractions to KCl and sodium fluoride in all situations.
0(0,0,0,0) Details
11414680 Berredo-Pinho M, Peres-Sampaio CE, Chrispim PP, Belmont-Firpo R, Lemos AP, Martiny A, Vannier-Santos MA, Meyer-Fernandes JR: A Mg-dependent ecto-ATPase in Leishmania amazonensis and its possible role in adenosine acquisition and virulence. Arch Biochem Biophys. 2001 Jul 1;391(1):16-24.

This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, furosemide, vanadate, molybdate, sodium fluoride, tartrate, and levamizole.
35(0,1,1,5) Details
15909416 Kravtsova VV, Kravtsov OV: [Inactivation of Na+, K+ -ATPase from cattle brain by sodium fluoride] . Ukr Biokhim Zh. 2004 Jan-Feb;76(1):39-47.

The influence of the physiological ligands and modifiers on the plasma membrane Na+, K+ -ATPase from calf brain inactivation by sodium fluoride (NaF) is studied.
119(1,2,2,9) Details
11051109 Meyer-Fernandes JR, Lanz-Mendoza H, Gondim KC, Willott E, Wells MA: Ectonucleotide diphosphohydrolase activities in hemocytes of larval Manduca sexta. Arch Biochem Biophys. 2000 Oct 1;382(1):152-9.

These ecto-ATPase activities were insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A1, ouabain, furosemide, vanadate, sodium fluoride, tartrate, and levamizole.
34(0,1,1,4) Details
16289087 Fonseca FV, Fonseca de Souza AL, Mariano AC, Entringer PF, Gondim KC, Meyer-Fernandes JR: Trypanosoma rangeli: characterization of a Mg-dependent ecto ATP-diphosphohydrolase activity. Exp Parasitol. 2006 Feb;112(2):76-84. Epub 2005 Nov 9.

This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A1, ouabain, furosemide, vanadate, molybdate, sodium fluoride, tartrate, and levamizole.
33(0,1,1,3) Details
11750998 Jesus JB, Lopes AH, Meyer-Fernandes JR: Characterization of an ecto-ATPase of Tritrichomonas foetus. Vet Parasitol. 2002 Jan 3;103(1-2):29-42.

The Mg (2+)-dependent ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, furosemide, vanadate, molybdate, sodium fluoride and levamizole.
33(0,1,1,3) Details
12209331 dos Passos Lemos A, de Sa Pinheiro AA, de Berredo-Pinho M, Fonseca de Souza L, Motta CM, de Souza W, Meyer-Fernandes R: Ectonucleotide diphosphohydrolase activity in Crithidia deanei. Parasitol Res. 2002 Oct;88(10):905-11. Epub 2002 Jun 19.

This ecto-ATPase activity was insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin A (1), ouabain, vanadate, molybdate, sodium fluoride and tartrate.
33(0,1,1,3) Details
16380977 Entringer PF, Gondim KC, Meyer-Fernandes JR: Ecto-nucleotidase activities in the fat body of Rhodnius prolixus. Arch Insect Biochem Physiol. 2006 Jan;61(1):1-9.

Both ecto-nucleotidase activities were insensitive to inhibitors of other ATPase and phosphatase activities, such as oligomycin, sodium azide, bafilomycin, ouabain, vanadate, molybdate, sodium fluoride, levamizole, tartrate, p-NPP, sodium phosphate, and suramin.
31(0,1,1,1) Details
9516436 Porter DJ: Inhibition of the hepatitis C virus helicase-associated ATPase activity by the combination of ADP, NaF, MgCl2, and poly (rU). J Biol Chem. 1998 Mar 27;273(13):7390-6.

8(0,0,0,8) Details
11544263 Reis M, Farage M, de Souza AC, de Meis L: Correlation between uncoupled ATP hydrolysis and heat production by the sarcoplasmic reticulum Ca2+-ATPase: coupling effect of fluoride. J Biol Chem. 2001 Nov 16;276(46):42793-800. Epub 2001 Sep 5.

6(0,0,0,6) Details
18832829 van Loveren C, Hoogenkamp MA, Deng DM, ten Cate JM: Effects of different kinds of fluorides on enolase and ATPase activity of a fluoride-sensitive and fluoride-resistant Streptococcus mutans strain. Caries Res. 2008;42(6):429-34. Epub 2008 Oct 3.

4(0,0,0,4) Details
10700386 Barros FS, De Menezes LF, Pinheiro AA, Silva EF, Lopes AH, De Souza W, Meyer-Fernandes JR: Ectonucleotide diphosphohydrolase activities in Entamoeba histolytica. Arch Biochem Biophys. 2000 Mar 15;375(2):304-14.

The Mg (2+)-independent ATPase activity was unaffected by pH in the range between 6.4 and 8. 4, in which the cells were viable.
Sodium orthovanadate, sodium fluoride, levamizole, and ammonium molybdate had no effect on the ATPase activities.
3(0,0,0,3) Details
17709908 Xu H, Zhou YL, Zhang JM, Liu H, Jing L, Li GS: Effects of fluoride on the intracellular free Ca2+ and Ca2+-ATPase of kidney. Biol Trace Elem Res. 2007 Jun;116(3):279-88.

In vivo, 48 Wistar rats were divided into 4 groups, and half of rats were treated with sodium fluoride (NaF) by drinking water (per liter of tap water containing 100 mg F-).
3(0,0,0,3) Details
10823668 Murao H, Sakagami N, Iguchi T, Murakami T, Suketa Y: Sodium fluoride increases intracellular calcium in rat renal epithelial cell line NRK-52E. Biol Pharm Bull. 2000 May;23(5):581-4.

Moreover, fluoride alone was deduced to enhance the activity of ER-type Ca2+-ATPase.
3(0,0,0,3) Details
9184168 Sankaran B, Bhagat S, Senior AE: Inhibition of P-glycoprotein ATPase activity by beryllium fluoride. . Biochemistry. 1997 Jun 3;36(22):6847-53.

3(0,0,0,3) Details
9016786 Chen HK, Yeh NH: The nucleolar phosphoprotein P130 is a GTPase/ATPase with intrinsic property to form large complexes triggered by F- and Mg2+. Biochem Biophys Res Commun. 1997 Jan 13;230(2):370-5.

3(0,0,0,3) Details
19060940 Kosenko EA, Solomadin IN, Marov NV, Venediktova NI, Pogosian AS, Kaminskii IuG: [Role of glycolysis and antioxidant enzymes in the toxicity of amyloid beta peptide Abeta25-35 to erythrocytes]. Bioorg Khim. 2008 Sep-Oct;34(5):654-60.

The erythrotoxicity of Abeta (25-35) was shown to increase two- to fourfold both in the absence of glucose in the incubation medium and upon the addition of sodium fluoride, an enolase inhibitor.
Potassium cyanide, a Cu,Zn-superoxide dismutase inhibitor, abolishes the toxic effect of Abeta (25-35) to erythrocytes, whereas mercaptosuccinate, a glutathione peroxidase inhibitor, and ouabain, a Na+,K+-ATPase inhibitor, promote it.
2(0,0,0,2) Details
9743476 Guilherme A, Reis-Silva C, Moraes-Albuquerque JH, Oliveira MM, Vieyra A: A novel phosphorylated lipid counteracts activation of the renal plasma membrane (Ca2+ + Mg2+) ATPase by endogenous phosphatidylinositol-4-phosphate. Biosci Rep. 1998 Apr;18(2):79-89.

2(0,0,0,2) Details
9371849 Hansson M, Kannangara CG: ATPases and phosphate exchange activities in magnesium chelatase subunits of Rhodobacter sphaeroides. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13351-6.


Magnesium ions were required for the ATPase activities of both BchH and BchI+D, and these activities were inhibited 50% by 2 mM o-phenanthroline.
2(0,0,0,2) Details
10066761 Nadanaciva S, Weber J, Senior AE: Binding of the transition state analog MgADP-fluoroaluminate to F1-ATPase. . J Biol Chem. 1999 Mar 12;274(11):7052-8.

2(0,0,0,2) Details
10727425 Marchesini N, Luo S, Rodrigues CO, Moreno SN, Docampo R: Acidocalcisomes and a vacuolar H+-pyrophosphatase in malaria parasites. Biochem J. 2000 Apr 1;347 Pt 1:243-53.

Plasmodium berghei trophozoites were loaded with the fluorescent calcium indicator, fura-2 acetoxymethyl ester, to measure their intracellular Ca (2+) concentration ([Ca (2+)](i)). [Ca (2+)](i) was increased in the presence of the sarcoplasmic/endoplasmic reticulum Ca (2+)-ATPase inhibitor, thapsigargin.
PP (i) hydrolysis was sensitive in a dose-dependent manner to AMDP, imidodiphosphate, sodium fluoride, dicyclohexylcarbodi-imide and to the thiol reagent, N-ethylmaleimide.
2(0,0,0,2) Details
9784495 Gdula DA, Sandaltzopoulos R, Tsukiyama T, Ossipow V, Wu C: Inorganic pyrophosphatase is a component of the Drosophila nucleosome remodeling factor complex. Genes Dev. 1998 Oct 15;12(20):3206-16.

The 140-kD NURF subunit, imitation switch (ISWI), is related to the SWI2/SNF2 ATPase.
Inhibition of the pyrophosphatase activity of NURF with sodium fluoride has no significant effect on chromatin remodeling, indicating that these two activities may be biochemically uncoupled.
1(0,0,0,1) Details