Protein Information

Name serum albumins
Synonyms ALB; Albumin; PRO0883; PRO0903; PRO1341; PRO1708; PRO2044; PRO2619…

Compound Information

Name methylene chloride
CAS dichloromethane

Reference List

PubMed Abstract RScore(About this table)
7730958 Latha MS, Jayakrishnan A: A new method for the synthesis of smooth, round, hydrophilic protein microspheres using low concentrations of polymeric dispersing agents. J Microencapsul. 1995 Jan-Feb;12(1):7-12.


The versatility of the method is demonstrated by the synthesis of albumin microspheres, as well as those of the amphiphilic protein casein, the latter being more difficult to prepare by surfactant stabilization techniques.
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10575628 Lin SY, Chen KS, Teng HH: Protective colloids and polylactic acid co-affecting the polymorphic crystal forms and crystallinity of indomethacin encapsulated in microspheres. J Microencapsul. 1999 Nov-Dec;16(6):769-76.

However, the amorphous and methylene chloride solvate of IMC seemed to exist in the IMC-loaded PLA microspheres prepared with the proteins (gelatin or albumin), synthetic cellulose derivative (methyl cellulose or hydroxylpropyl methylcellulose) or the synthetic nonionic polymer (polyvinyl alcohol, polyvinyl pyrrolidone or biosoluble polymer) as a protective colloid.
31(0,1,1,1) Details
9972501 Lin SY, Chen KS, Teng HS: Effect of protective colloids on the induction of polymorphic changes in indomethacin agglomerates after solvent evaporation from o/w emulsions. J Microencapsul. 1999 Jan-Feb;16(1):39-47.

There was only methylene chloride solvate of IMC with a small amount of amorphous form in the IMC agglomerates prepared from albumin as a protective colloid, while IMC agglomerates prepared from methylcellulose, polyvinyl alcohol or biosoluble polymer consisted of the mixture of amorphous and alpha forms, and methylene chloride solvate of IMC.
6(0,0,1,1) Details
9653765 Raghuvanshi RS, Goyal S, Singh O, Panda AK: Stabilization of dichloromethane-induced protein denaturation during microencapsulation. Pharm Dev Technol. 1998 May;3(2):269-76.


Of the many additives tested to overcome the DCM-induced denaturation of proteins, serum albumins and polyvinyl alcohol (PVA) showed promising results in terms of retention of the immunoreactivity of the protein.
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