Protein Information

Name Cl 2
Synonyms AMB11; MHC class I NK cell receptor; CD158E1; CD158E1/2; CD158E2; CL 11; CL 2; HLA BW4 specific inhibitory NK cell receptor…

Compound Information

Name methylene chloride
CAS dichloromethane

Reference List

PubMed Abstract RScore(About this table)
16423479 Nguelefack TB, Nana P, Atsamo AD, Dimo T, Watcho P, Dongmo AB, Tapondjou LA, Njamen D, Wansi SL, Kamanyi A: Analgesic and anticonvulsant effects of extracts from the leaves of Kalanchoe crenata (Andrews) Haworth (Crassulaceae). J Ethnopharmacol. 2006 Jun 15;106(1):70-5. Epub 2006 Jan 19.

In the present work, the analgesic effects of methylene chloride/methanol (1:1) (CH (2) Cl (2)/CH (3) OH) extract and its hexane, methylene chloride (CH (2) Cl (2)), ethyl acetate, n-butanol fractions and aqueous residue have been evaluated using acetic acid, formalin and pressure test.
12(0,0,1,7) Details
17250985 Dimo T, Mtopi OS, Nguelefack TB, Kamtchouing P, Zapfack L, Asongalem EA, Dongo E: Vasorelaxant effects of Brillantaisia nitens Lindau (Acanthaceae) extracts on isolated rat vascular smooth muscle. J Ethnopharmacol. 2007 Apr 20;111(1):104-9. Epub 2006 Nov 7.

The methylene chloride/methanol (CH (2) Cl (2)/CH (3) OH) extract was the most potent relaxing extract.
8(0,0,1,3) Details
11714364 Tachibana Y, Kikuzaki H, Lajis NH, Nakatani N: Antioxidative activity of carbazoles from Murraya koenigii leaves. J Agric Food Chem. 2001 Nov;49(11):5589-94.

The methylene chloride (CH (2) Cl (2)) extract and the ethyl acetate (EtOAc) soluble fraction of the 70% acetone extract significantly prolonged the OSI values comparable to those of alpha-tocopherol and BHT.
8(0,0,1,3) Details
16216298 Marquis O, Millery A, Guittonneau S, Miaud C: Solvent toxicity to amphibian embryos and larvae. Chemosphere. 2006 May;63(5):889-92. Epub 2005 Oct 10.

Tested solvents were methanol (MeOH), methylene chloride (CH (2) Cl (2)), dimethyl sulfoxyde (DMSO), acetone (Ac) and ethanol (EtOH).
7(0,0,1,2) Details
18606434 Ma X, Green SA: Fractionation and spectroscopic properties of fulvic acid and its extract. Chemosphere. 2008 Aug;72(10):1425-34. Epub 2008 Jul 7.

An aqueous fulvic acid solution was also extracted with methylene chloride (CH (2) Cl (2)) by continuous liquid-liquid extraction.
7(0,0,1,2) Details
19439825 Zhang K, Wang X, Jing D, Yang Y, Zhu M: Bionic electrospun ultrafine fibrous poly (L-lactic acid) scaffolds with a multi-scale structure. Biomed Mater. 2009 Jun;4(3):035004. Epub 2009 May 14.

PLLA was dissolved in dimethylformamide (DMF) and methylene chloride (CH (2) Cl (2)) mixed solvent with different ratios.
7(0,0,1,2) Details
11599967 Soldatov DV, Diamente PR, Ratcliffe CI, Ripmeester JA: (6,17-Dimethyl-8,15-diphenyldibenzo [b,i][1,4,8,11]-tetra-aza [14] annulenato ) nickel (II) in solids: two guest-free polymorphs and inclusion compounds with methylene chloride and fullerene (C (60))-carbon disulfide. Inorg Chem. 2001 Oct 22;40(22):5660-7.

The inclusion compound with methylene chloride, [NiL](CH (2) Cl (2)), is stable in air and thermally stable up to approximately 128 degrees C.
7(0,0,1,2) Details
18451517 Jung HA, Jeong DM, Chung HY, Lim HA, Kim JY, Yoon NY, Choi JS: Re-evaluation of the antioxidant prenylated flavonoids from the roots of Sophora flavescens. Biol Pharm Bull. 2008 May;31(5):908-15.

This was in contrast to the prenylated flavanones, sophoraflavanone G and kurarinone, which were isolated from the methylene chloride (CH (2) Cl (2)) fraction of the same source.
6(0,0,1,1) Details
18403145 Nguelefack-Mbuyo PE, Nguelefack TB, Dongmo AB, Afkir S, Azebaze AG, Dimo T, Legssyer A, Kamanyi A, Ziyyat A: Anti-hypertensive effects of the methanol/methylene chloride stem bark extract of Mammea africana in l-NAME-induced hypertensive rats. J Ethnopharmacol. 2008 May 22;117(3):446-50. Epub 2008 Mar 4.

AIM OF THE STUDY: The methanol/methylene chloride (CH (3) OH/CH (2) Cl (2)) extract from the stem bark of Mammea africana was showed to possess vasodilating effect in the presence and the absence of N (omega)-nitro-l-arginine methyl ester (l-NAME).
6(0,0,1,1) Details
17015034 Umadevi M, Suvitha A, Latha K, Rajkumar BJ, Ramakrishnan V: Spectral investigations of preferential solvation and solute-solvent interactions of 1,4-dimethylamino anthraquinone in CH2Cl2/C2H5OH mixtures. Spectrochim Acta A Mol Biomol Spectrosc. 2007 Jul;67(3-4):910-5. Epub 2006 Oct 2.


The optical absorption and IR spectra of 1,4-dimethylamino anthraquinone (1,4-DMAAQ) in CH (2) Cl (2)/C (2) H (5) OH mixtures have been investigated.
5(0,0,0,5) Details
17177172 Kim SJ, Jung YS, Yoon MY, Kim YC: Comparative effects of dimethylsulfoxide on metabolism and toxicity of carbon tetrachloride and dichloromethane. J Appl Toxicol. 2007 Jan-Feb;27(1):25-31.


Male mice were treated with DMSO (1, 2.5 or 5 ml kg (-1), i.p.) prior to challenge with dichloromethane (CH (2) Cl (2)) or carbon tetrachloride (CCl (4)).
4(0,0,0,4) Details
17245785 Li T, Lough AJ, Morris RH: An acidity scale of tetrafluoroborate salts of phosphonium and iron hydride compounds in [D2] dichloromethane. Chemistry. 2007;13(13):3796-803.


Equilibrium constants (K) for reactions between acids and the conjugate base forms of a number of phosphonium salts, [HPR3][BF4], and iron hydrides, [Fe (CO) 3H (PR3) 2][BF4], in CD (2) Cl (2) have been determined by means of 31P and 1H NMR spectroscopy at 20 degrees C.
4(0,0,0,4) Details
16918250 Watanabe K, Guengerich FP: Limited reactivity of formyl chloride with glutathione and relevance to metabolism and toxicity of dichloromethane. Chem Res Toxicol. 2006 Aug;19(8):1091-6.


Formyl chloride has been indirectly implicated as an intermediate in the oxidation of CH (2) Cl (2) and proposed to be a product of the oxidation of some other compounds.
3(0,0,0,3) Details
14727918 Marsch GA, Botta S, Martin MV, McCormick WA, Guengerich FP: Formation and mass spectrometric analysis of DNA and nucleoside adducts by S-(1-acetoxymethyl) glutathione and by glutathione S-transferase-mediated activation of dihalomethanes. Chem Res Toxicol. 2004 Jan;17(1):45-54.


The dihalomethane CH (2) Cl (2) is an industrial solvent of potential concern to humans because of its potential genotoxicity and carcinogenicity.
3(0,0,0,3) Details
17166560 Alapi T, Dombi A: Direct VUV photolysis of chlorinated methanes and their mixtures in an oxygen stream using an ozone producing low-pressure mercury vapour lamp. Chemosphere. 2007 Mar;67(4):693-701. Epub 2006 Dec 12.


The gas-phase photooxidations of CCl (4), CHCl (3), CH (2) Cl (2) and their binary mixtures in an O (2) stream were studied in a flow reactor under various experimental conditions using a low-pressure mercury lamp as light source covered with a high-purity silica sleeve being used.
2(0,0,0,2) Details
15828786 Kim H, Guiochon G: Thermodynamic studies of the solvent effects in chromatography on molecularly imprinted polymers. 3. Anal Chem. 2005 Apr 15;77(8):2496-504.

The organic solvents studied were acetonitrile (MeCN), methylene chloride, chloroform, and tetrahydrofuran (THF), all in the presence of the same concentration of acetic acid, used as an organic modifier.
The highest energy sites for Fmoc-L-Trp in MeCN are inactive in CH (2) Cl (2), CHCl (3), and THF, and only two types of sites were identified in these solvents.
2(0,0,0,2) Details
15236520 Liang HC, Henson MJ, Hatcher LQ, Vance MA, Zhang CX, Lahti D, Kaderli S, Sommer RD, Rheingold AL, Zuberbuhler AD, Solomon EI, Karlin KD: Solvent effects on the conversion of dicopper (II) micro-eta (2):eta (2)-peroxo to bis-micro-oxo dicopper (III) complexes: direct probing of the solvent interaction. Inorg Chem. 2004 Jul 12;43(14):4115-7.


UV-visible absorption and resonance Raman spectroscopic signatures demonstrate that the equilibrium is highly solvent dependent; the mixture is mostly 2 (Peroxo) in CH (2) Cl (2), but there are significantly increasing quantities of 2 (Oxo) along the series methylene chloride --> diethyl ether --> acetone --> tetrahydrofuran (THF).
1(0,0,0,1) Details
18703342 Chattopadhyay S, Das SS, Das MK, Goomer NC: Recovery of 99mTc from Na2 [99Mo] MoO4 solution obtained from reactor-produced (n,gamma) 99Mo using a tiny Dowex-1 column in tandem with a small alumina column. Appl Radiat Isot. 2008 Dec;66(12):1814-7. Epub 2008 Jul 4.

Low to medium specific activity (99) Mo-molybdate solution of 7.4-18.5GBq (200-500mCi) in sodium hydroxide was passed through a tiny Dowex-1 column (25mg) to separate the (99m) Tc from the (99) Mo; subsequently the (99m) Tc was eluted from the Dowex 1 column with tetrabutylammonium bromide (TBAB) solution (1mg/5ml methylene chloride).
The TBAB solution was passed through a small alumina column (1.5g) where the (99m) Tc is retained and separated from TBAB and CH (2) Cl (2).
1(0,0,0,1) Details
17076506 Yamamoto T, Fukushima T, Yamamoto Y, Kosaka A, Jin W, Ishii N, Aida T: Stabilization of a kinetically favored nanostructure: surface ROMP of self-assembled conductive nanocoils from a norbornene-appended hexa-peri-hexabenzocoronene. J Am Chem Soc. 2006 Nov 8;128(44):14337-40.


Newly designed norbornene-appended hexabenzocoronene 1 self-assembles, upon diffusion of an Et (2) O vapor into its CH (2) Cl (2) solution, to form either graphitic nanocoils or nanotubes, depending on the self-assembling conditions.
1(0,0,0,1) Details
18484769 Lin KW, Chen CY, Chen WF, Yan TH: A new entry to 1,5-keto esters and their 4,4-dideuterio derivatives via methylene chloride as "methylene dianion" equivalents. J Org Chem. 2008 Jun 20;73(12):4759-61. Epub 2008 May 17.

This TiCl 4-Mg promoted multicomponent coupling of various amides with CH (2) Cl (2) and methyl acrylate represents an extremely simple and practical synthesis of 1,5-keto esters.
1(0,0,0,1) Details
12005504 Chisholm MH, Gallucci J, Phomphrai K: Coordination chemistry and reactivity of monomeric alkoxides and amides of magnesium and zinc supported by the diiminato ligand CH (CMeNC (6) H (3)-2,6-(i) Pr (2))(2). Inorg Chem. 2002 May 20;41(10):2785-94.

Their dynamic solution behavior has been examined by variable-temperature NMR studies and reveals that THF reversibly dissociates in toluene-d (8) or CD (2) Cl (2) and that exchange with free THF occurs by a dissociative process.
The reactions proceed faster in methylene chloride, but for M = Mg, a Bernoulian distribution of tetrads is formed from rac-lactide (3iii:2isi:sii:sis:iis) prior to trans-esterification.
1(0,0,0,1) Details
18970685 Gierak A, Seredych M, Bartnicki A: The preparation, properties and application of carbon fibers for SPME. Talanta. 2006 Jul 15;69(5):1079-87. Epub 2006 Jan 19.

The conditions of preparation of new types of carbon fibers for solid phase micro extraction (SPME) prepared by methylene chloride pyrolysis (at 600 degrees C) on the quartz fiber (100 microm) as well as by supporting synthetic active carbon (prepared especially for this purposes) supported in a special epoxide-acrylic polymer is described.
In this paper, we present the analysis of BTX contents in the petrol analyzed with the application carbonized with CH (2) Cl (2) SPME fiber (C1NM) and a headspace over the petrol sample (concentration of each BTX approximately g/dm (3)).
1(0,0,0,1) Details
17396180 Kandratsenka A, Schroeder J, Schwarzer D, Vikhrenko VS: Mode-specific energy absorption by solvent molecules during CO2 vibrational cooling. Phys Chem Chem Phys. 2007 Apr 14;9(14):1688-92. Epub 2007 Feb 23.


Non-equilibrium molecular dynamics (NEMD) simulations of energy transfer from vibrationally excited CO (2) to CCl (4) and CH (2) Cl (2) solvent molecules are performed to identify the efficiency of different energy pathways into the solvent bath.
1(0,0,0,1) Details
17803257 Taniguchi A, Yoshiya T, Abe N, Fukao F, Sohma Y, Kimura T, Hayashi Y, Kiso Y: 'O-Acyl isopeptide method' for peptide synthesis: Solvent effects in the synthesis of Abeta1-42 isopeptide using 'O-acyl isodipeptide unit'. J Pept Sci. 2007 Dec;13(12):868-74.


In non-polar solvents such as CHCl (3) or CH (2) Cl (2), the side reaction was less likely to occur.
1(0,0,0,1) Details
17485848 Kametani S, Kojima-Yuasa A, Kikuzaki H, Kennedy DO, Honzawa M, Matsui-Yuasa I: Chemical constituents of cape aloe and their synergistic growth-inhibiting effect on Ehrlich ascites tumor cells. Biosci Biotechnol Biochem. 2007 May;71(5):1220-9. Epub 2007 May 7.


Ten compounds were isolated from the dichloromethane (CH (2) Cl (2)) extract that showed the strongest activity, and their structures were elucidated as aloe-emodin (1), p-hydroxybenzaldehyde (2), p-hydroxyacetophenone (3), pyrocatechol (4), 10-oxooctadecanoic acid (5), 10-hydroxyoctadecanoic acid (6), methyl 10-hydroxyoctadecanoate (7), 7-hydroxy-2,5-dimethylchromone (8), furoaloesone (9), and 2-acetonyl-8-(2-furoylmethyl)-7-hydroxy-5-methylchromone (10) based on MS and various NMR spectroscopic techniques.
1(0,0,0,1) Details
19250808 Begum NA, Mondal S, Basu S, Laskar RA, Mandal D: Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts. Colloids Surf B Biointerfaces. 2009 Jun 1;71(1):113-8. Epub 2009 Jan 21.


We identified these biomolecules as the tea polyphenols, including flavonoids, which were present in comparable amounts in both the tea leaf broth and ethyl acetate extract, but are absent in the CH (2) Cl (2) extract of tea leaves.
1(0,0,0,1) Details
11978105 Ghiladi RA, Karlin KD: Low-temperature UV-visible and NMR spectroscopic investigations of O (2) binding to ((6) L) Fe (II), a ferrous heme bearing covalently tethered axial pyridine ligands. Inorg Chem. 2002 May 6;41(9):2400-7.

Complex 1, or its pyrrole-deuterated analogue ((6) L-d (8)) Fe (II) (1-d (8)), exhibits downfield shifted pyrrole resonances (delta 28-60 ppm) in all solvents utilized [CH (2) Cl (2), (CH (3))(2) C (O), CH (3) CN, THF], indicative of a five-coordinate high-spin ferrous heme, even when there is no exogenous axial solvent ligand present (i.e., in methylene chloride).
Furthermore, ((6) L) Fe (II) (1) exhibits non-pyrrolic upfield and downfield shifted peaks in CH (2) Cl (2), (CH (3))(2) C (O), and CH (3) CN solvents, which we ascribed to resonances arising from the intra- or intermolecular binding of a TMPA-pyridyl arm to the ferrous heme.
1(0,0,0,1) Details
12005502 Bryant JR, Taves JE, Mayer JM: Oxidations of hydrocarbons by manganese (III) tris (hexafluoroacetylacetonate). Inorg Chem. 2002 May 20;41(10):2769-76.

In CH (2) Cl (2) solution, oxidation of DHA is catalyzed by chloride ion.
It forms stable solutions in benzene and methylene chloride but is rapidly reduced in acetonitrile, DMSO, acetone, and ethers.
1(0,0,0,1) Details