Protein Information

Name leptin
Synonyms LEP; Leptin; Leptin precursor; OB; OBS; Obese protein; Obesity factor; leptin (murine obesity homolog)…

Compound Information

Name boric acid
CAS

Reference List

PubMed Abstract RScore(About this table)
10733224 Richards MP, Ashwell CM, McMurtry JP: Quantitative analysis of leptin mRNA using competitive reverse transcription polymerase chain reaction and capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 2000 Mar;21(4):792-8.

Leptin amplicons were separated using a DB-1 coated capillary (27 cm x 100 microm ID) at a field strength of 300 V/cm in a replaceable sieving matrix consisting of 0.5% hydroxypropylmethyl cellulose (HPMC) in 1 x TBE (89 mM Tris-base, 89 mM boric acid, 2 mM EDTA, pH 8.3) buffer with 0.5 microg/mL EnhanCE fluorescent intercalating dye.
13(0,0,1,8) Details
10486739 Richards MP, Ashwell CM, McMurtry JP: Analysis of leptin gene expression in chickens using reverse transcription polymerase chain reaction and capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 1999 Aug 20;853(1-2):321-35.

Amplicons were separated using a DB-1 coated capillary (27 cm x 100 microns I.D.) at a field strength of 300 V/cm in a replaceable sieving matrix consisting of 0.5% hydroxypropylmethylcellulose (HPMC) in 1X TBE (89 mM Tris-base, 89 mM boric acid, 2 mM EDTA, pH 8.3) buffer with 0.5 microgram/ml EnhanCE fluorescent intercalating dye.
5(0,0,0,5) Details