Protein Information

Name ATPase
Synonyms ATP7A; MK; ATPase; Cation transporting ATPase; ATP7A protein; ATPase Cu(2+) transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1…

Compound Information

Name sodium thiocyanate
CAS sodium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
10413672 Agnew BJ, Duman JG, Watson CL, Coling DE, Forte JG: Cytological transformations associated with parietal cell stimulation: critical steps in the activation cascade. J Cell Sci. 1999 Aug;112 ( Pt 16):2639-46.


Immunofluorescence was used to localize the proton pump protein, H, K-ATPase, and the apical membrane-cytoskeletal linker protein, ezrin; fluorescent-labeled phalloidin was used as a marker of F-actin.
5(0,0,0,5) Details
129956 Ivaschenko AT, Ryskulova ST: [Bicarbonate-stimulated ATP-ase from membranes of rat erythrocytes] . Vopr Med Khim. 1975 Sep-Oct;21(5):492-4.

A bicarbonate-stimulating effect on the ATPase activity in membranes of rat erythrocytes depended upon pH, concentration of Mg2+ and HCO3-ions.
Sodium azide and sodium thiocyanate distinctly inhibited the ATPase activity; pentachlorphenol and 2,4-dinitrophenol did not change it.
2(0,0,0,2) Details
7977708 Thibodeau A, Kuo RC, Crothers JM Jr, Yao X, Owicki JC, Forte JG: Direct measurement of extracellular proton flux from isolated gastric glands. Am J Physiol. 1994 Nov;267(5 Pt 1):C1473-82.

The elevated EAR at steady state reflected liberation of metabolic acid attributed to H (+)-K (+)-ATPase enzymatic activity.
Sodium thiocyanate (1 mM), which short circuits intraluminal H+ accumulation, blocked OH- release, demonstrating its dependence on H (+)-OH- separation at the apical membrane.
2(0,0,0,2) Details
17242477 Kotwica J, Ciuk MA, Joachimiak E, Rowinski S, Cymborowski B, Bebas P: Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis (Lepidoptera: Noctuidae) controlled by circadian clock. J Physiol Pharmacol. 2006 Nov;57 Suppl 8:107-23.

This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase.
Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.
2(0,0,0,2) Details
7691365 Welsh NJ, Shankley NP, Black JW: Comparative study of the control of basal acid output from rodent isolated stomachs. Br J Pharmacol. 1993 Aug;109(4):941-5.

Isolated, lumen-perfused, whole stomach preparations from mouse and immature rat produced a stable basal acid output which, although not blocked by histamine H2-, acetylcholine M- or CCKB/gastrin receptor antagonists, was almostly completely blocked by the H+/K (+)-ATPase inhibitor, omeprazole, and the metabolic inhibitor, sodium thiocyanate (NaSCN). 2.
In the absence of suprathreshold endogenous histamine, it is argued that H+/K (+)-ATPase mediated basal acid secretion from the mouse stomach assay is regulated by something other than cyclic AMP.
1(0,0,0,1) Details
162573 Suzuki S: The presence and properties of Mg2+-HCO3 (-)-stimulated and SCN (-)-inhibited ATPase in mouse kidney and some relationships between ATPase and carbonic anhydrase. Comp Biochem Physiol B. 1978;59(1):27-36.

1(0,0,0,1) Details
2996300 Vega FV, Olaisson H, Mardh S: Distribution of carbonic anhydrase in cells and membranes isolated from pig gastric mucosa. Acta Physiol Scand. 1985 Aug;124(4):573-9.


Purified membranes from pig gastric mucosa, which contained the acid pump of the stomach, the H,K-ATPase, also contained a firmly bound carbonic anhydrase of high activity.
1(0,0,0,1) Details