Protein Information

Name protein a
Synonyms A1 activator; PSAP; Protein C; CSAct; Cerebroside sulfatase activator; Cerebroside sulfate activator; Co beta glucosidase; Component C…

Compound Information

Name sodium thiocyanate
CAS sodium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
659858 Mackenzie MR, Warner NL, Mitchell GF: The binding of murine immunoglobulins to staphylococcal protein A. . J Immunol. 1978 May;120(5):1493-6.

Differential elution by sodium thiocyanate gradients is described.
1(0,0,0,1) Details
116576 Warr GW, Hart IR: Binding of canine IgM and IgG to protein A of Staphylococcus aureus: a simple method for the isolation of canine immunoglobulins from serum and the lymphocyte surface. Am J Vet Res. 1979 Jul;40(7):922-6.

Attempts to elute differentially IgG and IgM from protein A-Sepharose columns, using gradients of pH or the chaotropic agent sodium thiocyanate, were unsuccessful.
9(0,0,1,4) Details
18771565 Kavanagh-Wright L, Smith TP, Gibney J, McKenna TJ: Characterization of macroprolactin and assessment of markers of autoimmunity in macroprolactinaemic patients. Clin Endocrinol. 2009 Apr;70(4):599-605. Epub 2008 Sep 2.

DESIGN: PRL was measured in sera prior to and following gel filtration chromatography, ultrafiltration, treatment with protein A-sepharose, protein G-sepharose, antihuman IgG-agarose and sodium thiocyanate (NaSCN).
6(0,0,1,1) Details
108802 Chalon MP, Milne RW, Vaerman JP: Interactions between mouse immunoglobulins and staphylococcal protein A. . Scand J Immunol. 1979;9(4):359-64.

When a gradient from 0 to 3 M sodium thiocyanate was started after the major peak of unbound proteins, all IgG1 was eluted before IgG2 and IgG3.
3(0,0,0,3) Details
3748998 Jenny R, Church W, Odegaard B, Litwiller R, Mann K: Purification of six human vitamin K-dependent proteins in a single chromatographic step using immunoaffinity columns. Prep Biochem. 1986;16(3):227-45.

Monoclonal antibodies to Factor VII, Factor IX, Factor X, Protein C, and Protein S were prepared from mice immunized with isolated vitamin K-dependent antigens.
The proteins were eluted from the columns by sodium thiocyanate and retained functional activity following dialysis.
2(0,0,0,2) Details