Protein Information

Name protein is
Synonyms ANX 2; p36; LIP 2; LIP2; ANX2; ANX2L4; ANX2P1; ANX2P2…

Compound Information

Name sodium thiocyanate
CAS sodium thiocyanate

Reference List

PubMed Abstract RScore(About this table)
731797 Ito J: Bacteriophage phi29 terminal protein: its association with the 5' termini of the phi29 genome. J Virol. 1978 Dec;28(3):895-904.

The protein is invariably associated with the two terminal DNA fragments generated by restriction endonucleases.
The protein remains associated with the phi29 DNA after treatment with various chaotropic agents, including 8 M urea, 6 M guanidine-hydrochloride, 4 M sodium perchlorate, 2 M sodium thiocyanate, and 2 M LiCl.
2(0,0,0,2) Details
9774689 Achsel T, Ahrens K, Brahms H, Teigelkamp S, Luhrmann R: The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein. Mol Cell Biol. 1998 Nov;18(11):6756-66.

Native U5 proteins were obtained by dissociation of U5 snRNP particles with the chaotropic salt sodium thiocyanate.
By cDNA cloning, we show that the 40kD protein is a novel WD-40 repeat protein and is thus likely to mediate regulated protein-protein interactions.
2(0,0,0,2) Details
9187368 Karnauchov I, Herrmann RG, Klosgen RB: Transmembrane topology of the Rieske Fe/S protein of the cytochrome b6/f complex from spinach chloroplasts. FEBS Lett. 1997 May 19;408(2):206-10.


The protein is resistant to membrane extraction by 0.1 M Na2CO3 or 2 M NaBr but is quantitatively released by 0.1 M NaOH.
2(0,0,0,2) Details
6333980 Kodama T, Miyauchi T, Okano T, Fuse H, Akimoto S, Shimazaki J: Sex steroid-binding protein in a subline of Dunning R 3327 prostatic adenocarcinoma of the rat. Endocrinol Jpn. 1984 Jun;31(3):301-9.

The sedimentation coefficient of the protein is 10S.
Sodium thiocyanate inhibits the binding to both sex steroids.
1(0,0,0,1) Details
659858 Mackenzie MR, Warner NL, Mitchell GF: The binding of murine immunoglobulins to staphylococcal protein A. . J Immunol. 1978 May;120(5):1493-6.

The binding of murine IgG1, IgG2a, IgG2b, IgG3 protein molecules to Staphylococcal A protein is presented.
Differential elution by sodium thiocyanate gradients is described.
1(0,0,0,1) Details
19478453 dos Santos CR, Fessel MR, Vieira Lde C, Krieger MA, Goldenberg S, Guimaraes BG, Zanchin NI, Barbosa JA: Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1;65(Pt 6):641-4. Epub 2009 May 23.

Gene-expression analysis indicates that this protein is differentially expressed during the T. cruzi life cycle, suggesting that it plays an important role during T. cruzi development.
A diffraction data set was collected to 1.50 A resolution from a single crystal grown in 25% PEG 1500, 200 mM sodium thiocyanate pH 6.9, 10 mM phenol and 10% ethylene glycol.
1(0,0,0,1) Details
3102336 Lax ER, Peetz A, Schriefers H: Effect of gonadectomy and exogenous sex hormone administration on the concentrations of hepatic oestrogen receptors and hepatic atypical sex hormone binding protein (HASP) in the rat. Horm Metab Res. 1986 Dec;18(12):814-7.


Oestradiol binding to this protein can be prevented by inclusion of sodium thiocyanate in the assay buffer, thereby permitting unhindered measurement of the oestrogen receptor concentrations.
0(0,0,0,0) Details
4063396 Kon OL: Antiestrogen binding sites in rat liver nuclei. . Biochim Biophys Acta. 1985 Dec 13;843(3):245-53.


The nuclear antiestrogen binding protein is not solubilized by 0.6 M potassium chloride, 2 M sodium chloride, 0.6 M sodium thiocyanate, 3 M urea, 20 mM pyridoxal phosphate, 1% (w/v) digitonin or 2% (w/v) sodium cholate but is extractable by sonication, indicating that it is tightly bound within the nucleus.
0(0,0,0,0) Details