Protein Information

Name diaphorase
Synonyms Diaphorase; DHQU; DIA 4; DIA4; DT diaphorase; DTD; Diaphorase (NADH/NADPH); Diaphorase (NADH/NADPH) (cytochrome b 5 reductase)…

Compound Information

Name nicotine
CAS

Reference List

PubMed Abstract RScore(About this table)
19960323 Badoer E: Retrogradely transported neuronal tracers combined with immunohistochemistry using free-floating brain sections. Methods Mol Biol. 2010;611:73-85.

In this chapter, we will focus on protocols that we use to combine the detection of (i) Fos-positive neurons to detect increased neuronal activity, (ii) nicotine adenine dinucleotide phosphate-diaphorase (NADPH-d) to detect nitric oxide synthase, and (iii) retrogradely transported tracers to identify specific projections.
31(0,1,1,1) Details
19303431 Ichikawa H, Terayama R, Yamaai T, Sugimoto T: Peptide 19 in the rat superior cervical ganglion. Neuroscience. 2009 Jun 16;161(1):86-94. Epub 2009 Mar 19.

In contrast, the treatment induced nicotine adenine dinucleotide phosphate diaphorase activity in 12.7% of postganglionic sympathetic neurons.
12(0,0,2,2) Details
19255787 Roszer T, Jozsa T, Szentmiklosi AJ, Banfalvi G: Acetylcholine inhibits nitric oxide (NO) synthesis in the gastropod nervous system. Cell Tissue Res. 2009 May;336(2):325-35. Epub 2009 Mar 3.

Analyses by the NADPH-diaphorase reaction, immunocytochemistry, purification with ion-exchange chromatography, Western-blot, and quantitative polymerase chain reaction have revealed the expression of neuronal NOS in the rat intestine and of a 60-kDa subunit of NOS in the enteric nerve plexus of H. pomatia.
In rats, ACh moderately elevates nitrite production, whereas ACh, the nicotinic receptor agonists (nicotine, acetyl thiocholine iodide, metacholine) and the cholinesterase inhibitor eserine reduce enteric nitrite formation in snails.
1(0,0,0,1) Details