| Name | beta glucuronidase |
|---|---|
| Synonyms | Beta glucuronidase; Beta G1; Beta glucuronidase precursor; GUSB; MPS 7; MPS7; Beta G1s; Beta glucuronidase precursors |
| Name | benzyladenine |
|---|---|
| CAS |
| PubMed | Abstract | RScore(About this table) | |
|---|---|---|---|
| 8453300 | Uknes S, Dincher S, Friedrich L, Negrotto D, Williams S, Thompson-Taylor H, Potter S, Ward E, Ryals J: Regulation of pathogenesis-related protein-1a gene expression in tobacco. Plant Cell. 1993 Feb;5(2):159-69. Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene. |
1(0,0,0,1) | Details |
| 16652220 | Sudan C, Prakash S, Bhomkar P, Jain S, Bhalla-Sarin N: Ubiquitous presence of beta-glucuronidase (GUS) in plants and its regulation in some model plants. Planta. 2006 Sep;224(4):853-64. Epub 2006 Apr 28. |
2(0,0,0,2) | Details |
| 15133712 | Manickavasagam M, Ganapathi A, Anbazhagan VR, Sudhakar B, Selvaraj N, Vasudevan A, Kasthurirengan S: Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane species hybrids) using axillary buds. Plant Cell Rep. 2004 Sep;23(3):134-43. Epub 2004 May 5. Axillary bud explants from 6-month-old sugarcane cultivars Co92061 and Co671 were co-cultivated with Agrobacterium strains LBA4404 and EHA105 that harboured a binary vector pGA492 carrying neomycin phosphotransferase II, phosphinothricin acetyltransferase (bar) and an intron containing beta-glucuronidase (gus-intron) genes in the T-DNA region. Transgenic plants were generated in three different steps: (1) production of putative primary transgenic shoots in Murashige-Skoog (MS) liquid medium with 3.0 mg l (-1) 6-benzyladenine (BA) and 5.0 mg l (-1) PPT, (2) production of secondary transgenic shoots from the primary transgenic shoots by growing them in MS liquid medium with 2.0 mg l (-1) BA, 1.0 mg l (-1) kinetin (Kin), 0.5 mg l (-1) alpha-napthaleneacetic acid (NAA) and 5.0 mg l (-1) PPT for 3 weeks, followed by five more cycles of shoot proliferation and selection under same conditions, and (3) rooting of transgenic shoots on half-strength MS liquid medium with 0.5 mg l (-1) NAA and 5.0 mg l (-1) PPT. |
2(0,0,0,2) | Details |
| 12635705 | Soniya EV, Das MR: In vitro organogenesis and genetic transformation in popular Cucumis sativus L. through Agrobacterium tumefaciens. Indian J Exp Biol. 2002 Mar;40(3):329-33. Organogenesis was induced directly without any intervening callus phase on Murashige and Skoog medium supplemented with different concentrations of benzyladenine and Sheetal, plants by infecting cotyledonary explants with Agrobacterium tumefaciens strain LBA4404 carrying binary plasmid pBI121, which contains scorable marker, beta-glucuronidase and selectable marker nptII under the CaMV 35S promoter. |
1(0,0,0,1) | Details |
| 9375416 | Okkels FT, Ward JL, Joersbo M: Synthesis of cytokinin glucuronides for the selection of transgenic plant cells. Phytochemistry. 1997 Nov;46(5):801-4. derivatives of cytokinins have been synthesized for use as agents for the selection of plant cells transformed with a beta-glucuronidase (GUS) gene. |
1(0,0,0,1) | Details |
| 16113211 | Welchen E, Gonzalez DH: Differential expression of the Arabidopsis cytochrome c genes Cytc-1 and Cytc-2. Plant Physiol. 2005 Sep;139(1):88-100. Epub 2005 Aug 19. The promoters of the Arabidopsis (Arabidopsis thaliana) cytochrome c genes, Cytc-1 and Cytc-2, were analyzed using plants transformed with fusions to the beta-glucuronidase coding sequence. |
1(0,0,0,1) | Details |
| 14615906 | Akutsu M, Ishizaki T, Sato H: Transformation of the monocotyledonous Alstroemeria by Agrobacterium tumefaciens. Plant Cell Rep. 2004 Mar;22(8):561-8. Epub 2003 Nov 13. These plasmids contain the beta-glucuronidase gene ( gusA) as a reporter gene and the hygromycin phosphotransferase and neomycin phosphotransferase II ( nptII) genes as selective markers. Efficient shoot regeneration from the transformed calli was observed on half-strength MS medium supplemented with 0.5 mg/l naphthaleneacetic acid and 0.5 mg/l benzyladenine after about 5 months of culture. |
1(0,0,0,1) | Details |
| 9002604 | Dai Z, Gao J, An K, Lee JM, Edwards GE, An G: Promoter elements controlling developmental and environmental regulation of a tobacco ribosomal protein gene L34. Plant Mol Biol. 1996 Dec;32(6):1055-65. A 1500 bp upstream promoter fragment was fused to the chloramphenicol acetyltransferase (CAT) reporter gene or beta-glucuronidase (GUS) reporter gene and transferred into tobacco plants by the Agrobhacterium-mediated leaf disk transformation method. Analysis of CAT activity in leaf tissues showed that mechanical wounding increased the rpL34 promoter activity about 5 times as compared to untreated controls and that the promoter activity was further enhanced by plant growth regulators, 2,4-dichlorophenoxyacetic acid and benzyladenine. |
1(0,0,0,1) | Details |
| 8790289 | Klotz KL, Lagrimini LM: Phytohormone control of the tobacco anionic peroxidase promoter. Plant Mol Biol. 1996 Jun;31(3):565-73. Three kb of the peroxidase promoter was joined to the coding region of the Escherichia coli beta-glucuronidase gene (GUS), and transiently expressed in tobacco mesophyll protoplasts in the presence or absence of plant growth regulators. Benzyladenine, ethylene, and did not affect peroxidase gene expression. |
1(0,0,0,1) | Details |
| 15815929 | Saini R, Jaiwal PK: Transformation of a recalcitrant grain legume, Vigna mungo L. Plant Cell Rep. 2005 Jun;24(3):164-71. Epub 2005 Apr 7. Hepper transformation was significantly increased from an average of 1% to 6.5% by using shoot apices excised from embryonic axes precultured on 10 microM benzyl- (BAP) for 3 days and wounded prior to inoculation in Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA2301, which contains a neomycin phosphotransferase gene (nptII) and a beta-glucuronidase (GUS) gene (gusA) interrupted by an intron. |
1(0,0,0,1) | Details |
| 12481063 | Hsieh MH, Goodman HM: Molecular characterization of a novel gene family encoding ACT domain repeat proteins in Arabidopsis. Plant Physiol. 2002 Dec;130(4):1797-806. Levels of ACR3 and ACR4 mRNA are increased by treatment with benzyladenine. |
0(0,0,0,0) | Details |
| 18057938 | Facchini PJ, Loukanina N, Blanche V: Genetic transformation via somatic embryogenesis to establish herbicide-resistant opium poppy. Plant Cell Rep. 2008 Apr;27(4):719-27. Epub 2007 Dec 5. Transformation was mediated by Agrobacterium tumefaciens using the pCAMBIA3301 vector, which harbors the phosphinothricin acetyltransferase (pat) gene driven by a tandem repeat of the cauliflower mosaic virus (CaMV) 35S promoter and the beta-glucuronidase (gus) structural gene driven by a single copy of the CaMV 35S promoter between left- and right-border sequences. Herbicide-resistant, proliferating callus was obtained from explants on a medium containing both 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA). |
1(0,0,0,1) | Details |
| 14770264 | Tsugawa H, Kagami T, Suzuki M: High-frequency transformation of Lobelia erinus L. by Agrobacterium-mediated gene transfer. Plant Cell Rep. 2004 May;22(10):759-64. Epub 2004 Feb 10. Leaf or cotyledon discs were inoculated with Agrobacterium tumefaciens strain EHA105 harboring the binary vector plasmid pIG121Hm, which contains a beta-glucuronidase gene with an intron as a reporter gene and both the neomycin phosphotransferase II and hygromycin phosphotransferase genes as selectable markers. The hygromycin-resistant calli produced on the selection medium were transferred to MS medium supplemented with 0.5 mg/l benzyladenine and 0.2 mg/l for regeneration of adventitious shoots. |
1(0,0,0,1) | Details |