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Magdalan J, Piotrowska A, Gomulkiewicz A, Sozanski T, Podhorska-Okolow M, Szelag A, Dziegiel P: Benzylpenicyllin and acetylcysteine protection from alpha-amanitin-induced apoptosis in human hepatocyte cultures. Exp Toxicol Pathol. 2010 Mar 4.
Viability of cultured hepatocytes was assessed using the MTT assay, whereas apoptosis processes were evaluated by the electron microscopy, detection of DNA laddering, determination of caspase-3 activity, and measuring annexin V, p53 and Bcl-2 protein concentration. |
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