Protein Information

Name nitrilase (protein family or complex)
Synonyms Nitrilase; Nitrilases

Compound Information

Name IAA
CAS

Reference List

PubMed Abstract RScore(About this table)
11525507 Vorwerk S, Biernacki S, Hillebrand H, Janzik I, Muller A, Weiler EW, Piotrowski M: Enzymatic characterization of the recombinant Arabidopsis thaliana nitrilase subfamily encoded by the NIT2/NIT1/NIT3-gene cluster. Planta. 2001 Mar;212(4):508-16.

7(0,0,0,7) Details
12972653 Park WJ, Kriechbaumer V, Moller A, Piotrowski M, Meeley RB, Gierl A, Glawischnig E: The Nitrilase ZmNIT2 converts indole-3-acetonitrile to indole-3-acetic acid. Plant Physiol. 2003 Oct;133(2):794-802. Epub 2003 Sep 4.

4(0,0,0,4) Details
11967096 Kutz A, Muller A, Hennig P, Kaiser WM, Piotrowski M, Weiler EW: A role for nitrilase 3 in the regulation of root morphology in sulphur-starving Arabidopsis thaliana. Plant J. 2002 Apr;30(1):95-106.

4(0,0,0,4) Details
11089910 Dohmoto M, Tsunoda H, Isaji G, Chiba R, Yamaguchi K: Genes encoding nitrilase-like proteins from tobacco. DNA Res. 2000 Oct 31;7(5):283-9.

4(0,0,0,4) Details
9368415 Normanly J, Grisafi P, Fink GR, Bartel B: Arabidopsis mutants resistant to the auxin effects of indole-3-acetonitrile are defective in the nitrilase encoded by the NIT1 gene. Plant Cell. 1997 Oct;9(10):1781-90.

3(0,0,0,3) Details
18182427 Kriechbaumer V, Park WJ, Piotrowski M, Meeley RB, Gierl A, Glawischnig E: Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis. J Exp Bot. 2007;58(15-16):4225-33.

3(0,0,0,3) Details
10412905 Quirino BF, Normanly J, Amasino RM: Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes. Plant Mol Biol. 1999 May;40(2):267-78.


The mRNA levels of a diverse array of gene products increases during leaf senescence, including a protease, a ribosomal protein, two cinnamyl alcohol dehydrogenases, a nitrilase and glyoxalase II.
2(0,0,0,2) Details
10574458 Dohmoto M, Sano J, Tsunoda H, Yamaguchi K: Structural analysis of the TNIT4 genes encoding nitrilase-like protein from tobacco. DNA Res. 1999 Oct 29;6(5):313-7.

2(0,0,0,2) Details
12430025 Pollmann S, Muller A, Piotrowski M, Weiler EW: Occurrence and formation of indole-3-acetamide in Arabidopsis thaliana. Planta. 2002 Nov;216(1):155-61. Epub 2002 Nov 12.


Recombinant nitrilase isoforms 1, 2 and 3, known to synthesize IAA from indole-3-acetonitrile, were shown to produce significant amounts of IAM in vitro as a second end product of the reaction besides IAA.
2(0,0,0,2) Details
16807824 Pollmann S, Muller A, Weiler EW: Many roads lead to "auxin": of nitrilases, synthases, and amidases. Plant Biol. 2006 May;8(3):326-33.

Recent progress in understanding the biosynthesis of the auxin, indole-3-acetic acid (IAA) in Arabidopsis thaliana is reviewed.
1(0,0,0,1) Details
12464638 Zhao Y, Hull AK, Gupta NR, Goss KA, Alonso J, Ecker JR, Normanly J, Chory J, Celenza JL: Trp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3. Genes Dev. 2002 Dec 1;16(23):3100-12.


Together with previous work on YUCCA, a flavin monooxygenase also implicated in IAOx production, and nitrilases that convert indole-3-acetonitrile to auxin, this work provides a framework for further dissecting auxin biosynthetic pathways and their regulation.
1(0,0,0,1) Details
16025407 Bennett RN, Wenke T, Freudenberg B, Mellon FA, Ludwig-Muller J: The tu8 mutation of Arabidopsis thaliana encoding a heterochromatin protein 1 homolog causes defects in the induction of secondary metabolite biosynthesis. Plant Biol. 2005 Jul;7(4):348-57.


Transcript levels known to be involved in IAA/glucosinolate synthesis and metabolism (nitrilase and myrosinase) were examined and both showed developmental regulation, while only nitrilase mRNA levels differed between wild type and mutant seedlings.
1(0,0,0,1) Details
12624772 Aloni R, Schwalm K, Langhans M, Ullrich CI: Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis. Planta. 2003 Mar;216(5):841-53. Epub 2002 Nov 26.


The mRNA expression of key enzymes involved in the synthesis, conjugate hydrolysis, accumulation and basipetal transport of auxin, namely indole-3-glycerol-phosphate-synthase, nitrilase, IAA-amino acid hydrolase, chalcone synthase and PIN1 as an essential component of the basipetal IAA carrier, was investigated by reverse transcription-polymerase chain reaction.
1(0,0,0,1) Details
16807825 Kriechbaumer V, Park WJ, Gierl A, Glawischnig E: Auxin biosynthesis in maize. Plant Biol. 2006 May;8(3):334-9.


Two nitrilases ZmNIT1 and ZmNIT2 are expressed in seeds.
1(0,0,0,1) Details
17555270 Idris EE, Iglesias DJ, Talon M, Borriss R: Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. Mol Plant Microbe Interact. 2007 Jun;20(6):619-26.


In addition, in the trp auxotrophic strains E101 (deltatrpBA) and E102 (deltatrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (deltaysnE, putative IAA transacetylase) and E105 (deltayhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished.
1(0,0,0,1) Details
11030425 Muller A, Weiler EW: IAA-synthase, an enzyme complex from Arabidopsis thaliana catalyzing the formation of indole-3-acetic acid from (S)-tryptophan. Biol Chem. 2000 Aug;381(8):679-86.


This, as well as immunological evidence for the presence of a nitrilase-like protein in the complex, suggests the reaction to proceed via the intermediate indole-3-acetonitrile.
1(0,0,0,1) Details
12060252 Ludwig-Muller J, Cohen JD: Identification and quantification of three active auxins in different tissues of Tropaeolum majus. Physiol Plant. 2002 Jun;115(2):320-329.


The presence of a nitrilase gene family and nitrilase activity in extracts from T. majus suggests that PAA might be synthesized by the nitrilase pathway using benzylglucosinolate as precursor.
1(0,0,0,1) Details