Protein Information

Name 26S proteasome (protein family or complex)
Synonyms 26 proteasome; 26S Proteasome; 26S protease; 26S proteases

Compound Information

Name jasmonic acid
CAS

Reference List

PubMed Abstract RScore(About this table)
17158585 Moon J, Zhao Y, Dai X, Zhang W, Gray WM, Huq E, Estelle M: A new CULLIN 1 mutant has altered responses to hormones and light in Arabidopsis. Plant Physiol. 2007 Feb;143(2):684-96. Epub 2006 Dec 8.


Ubiquitin ligases recognize and ubiquitinate target proteins for subsequent degradation by the 26S proteasome.
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20025249 Wasternack C, Kombrink E: Jasmonates: structural requirements for lipid-derived signals active in plant stress responses and development. ACS Chem Biol. 2010 Jan 15;5(1):63-77.

Enzymes participating in biosynthesis of jasmonic acid (JA) (1, 2) and components of JA signaling have been extensively characterized by biochemical and molecular-genetic tools.
The emerging picture of JA perception and signaling cascade implies the SCF (COI1) complex operating as E3 ubiquitin ligase that upon binding of JA-Ile targets JAZ repressors for degradation by the 26S-proteasome pathway, thereby allowing the transcription factor MYC2 to activate gene expression.
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19717617 Yan J, Zhang C, Gu M, Bai Z, Zhang W, Qi T, Cheng Z, Peng W, Luo H, Nan F, Wang Z, Xie D: The Arabidopsis CORONATINE INSENSITIVE1 protein is a jasmonate receptor. . Plant Cell. 2009 Aug;21(8):2220-36. Epub 2009 Aug 28.


CORONATINE INSENSITIVE1 (COI1), an F-box protein essential for all the jasmonate responses, interacts with multiple proteins to form the SCF (COI1) E3 ubiquitin ligase complex and recruits jasmonate ZIM-domain (JAZ) proteins for degradation by the 26S proteasome.
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19796781 Memelink J: Regulation of gene expression by jasmonate hormones. Phytochemistry. 2009 Sep;70(13-14):1560-70. Epub 2009 Sep 30.


The bioactive jasmonate (+)-7-iso-JA-l-Ile promotes the interaction between the ubiquitin ligase complex SCF (COI1) and JAZ proteins, resulting in their degradation by the 26S proteasome, thereby liberating AtMYC2 from repression according to the prevailing model.
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18713391 Zhu Z, Guo H: Genetic basis of ethylene perception and signal transduction in Arabidopsis. J Integr Plant Biol. 2008 Jul;50(7):808-15.

Ethylene has been shown to also regulate many other hormones' signaling pathways including auxin, abscisic acid and jasmonic acid, implying the existence of complicated signaling networks in the growth, development and defense responses of various plants.
In the absence of ethylene signal, EIN3 protein is degraded by an SCF complex containing one of the two F-box proteins EBF1/EBF2 in a 26S proteasome-dependent manner.
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19025383 Browse J: Jasmonate passes muster: a receptor and targets for the defense hormone. Annu Rev Plant Biol. 2009;60:183-205.


JA-Ile relieves repression by promoting binding of the JAZ proteins to the F-box protein CORONATINE INSENSITIVE1 (COI1) and their subsequent degradation by the ubiquitination/26S-proteasome pathway.
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17637677 Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J: JAZ repressor proteins are targets of the SCF (COI1) complex during jasmonate signalling. Nature. 2007 Aug 9;448(7154):661-5. Epub 2007 Jul 18.


Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities of the SCF (COI1) ubiquitin ligase and the 26S proteasome.
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18261950 Staswick PE: JAZing up jasmonate signaling. Trends Plant Sci. 2008 Feb;13(2):66-71. Epub 2008 Feb 7.

Jasmonate promotes interaction between JAZ proteins and the SCF (COI1) ubiquitin ligase, leading to JAZ degradation via the 26S proteasome in Arabidopsis thaliana.
Although jasmonic acid and methyl jasmonate were thought to be key regulators of jasmonate responses, they were ineffective in promoting SCF (COI1)-JAZ interaction and it is the isoleucine conjugate of jasmonic acid that acts in this signal transduction pathway.
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