| 14743538 |
Koval' OA, Boguslavskii EG, Oleinikova SB, Chernolovskaia EL, Litvak VV, Nadolinnyi VA, Blasov VV: [Copper-catalyzed cleavage of DNA by arenes] . Free Radic Biol Med. 2007 Jun 15;42(12):1851-7. Epub 2007 Mar 24.
DNA was found to be cleaved in neutral solutions containing arenes and copper (II) salts. The reaction is comparable in efficiency with the DNA cleavage by such systems as Cu (II)-phenanthroline and Cu (II)-ascorbic acid, but, in contrast to the latter, the system Cu (2+)-arene does not require the presence of an exogenous reducing agent or hydrogen peroxide. The system Cu (2+)-arene does not cleave DNA under anaerobic conditions. Catalase, sodium azide, and bathocuproine, which is a specific chelator of Cu (I), completely inhibit the reaction. The data obtained allow one to suppose that Cu (I) ions, superoxide radical, and singlet oxygen participate in the reaction. It has been shown by the EPR method using spin traps that the reaction proceeds with formation of alkoxyl radicals, which can insert breaks in the DNA molecule. For effective cleavage of DNA in the Cu (II)-o-bromobenzoic acid system, the radicals have to be generated by a specific copper-DNA-o-bromobenzoic acid complex, in which copper ions are most probably coordinated with oxygen atoms of the DNA phosphate groups. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru. |
31(0,1,1,1) |